公开(公告)号：US07648838B2

公开(公告)日：2010-01-19

申请号：US11267005

申请日：2005-11-04

申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

IPC分类号： G01N33/48 ,  G01N33/20 ,  G01N1/00

CPC分类号： C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/308

摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.

摘要翻译： 提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。 根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。 此外，由于可以使用各种固体支持物，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

公开(公告)号：US20060099711A1

公开(公告)日：2006-05-11

申请号：US11267005

申请日：2005-11-04

申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

IPC分类号： G01N1/00

CPC分类号： C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/308

摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.

摘要翻译： 提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。 根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。 此外，由于可以使用各种固体支持体，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

公开(公告)号：US20060134777A1

公开(公告)日：2006-06-22

申请号：US11304083

申请日：2005-12-15

申请人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

发明人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

IPC分类号： G01N27/447 ,  C12M1/33

CPC分类号： C12N1/066 ,  C12M47/06

摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

公开(公告)号：US07488596B2

公开(公告)日：2009-02-10

申请号：US11304083

申请日：2005-12-15

申请人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

发明人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

IPC分类号： C12M1/33 ,  C12M1/34 ,  C12Q1/70 ,  C12N1/06

CPC分类号： C12N1/066 ,  C12M47/06

摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.

摘要翻译： 提供了一种微流体装置，其包括用于细胞裂解的电解装置，其包括阳极室，阴极室和隔板，其中隔膜安装在阳极室和阴极室之间，阳极室包括入口和出口 阳极室溶液和电极，阴极室包括用于阴极室溶液和电极的入口和出口，以及使用其进行电化学裂解细胞的方法。

公开(公告)号：US07598064B2

公开(公告)日：2009-10-06

申请号：US12349273

申请日：2009-01-06

申请人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

发明人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Hee-kyun Lim ,  Kyu-youn Hwang ,  Soo-min Ma ,  Jun-hong Min

IPC分类号： C12N13/00 ,  C12N1/06 ,  C12M1/33 ,  C12M1/34 ,  C12Q1/70

CPC分类号： C12N1/066 ,  C12M47/06

摘要： Provided are a microfluidic device including an electrolysis device for cell lysis which includes an anode chamber, a cathode chamber and a separator, in which the separator is installed between the anode chamber and the cathode chamber, the anode chamber includes an inlet and an outlet for an anode chamber solution and an electrode, and the cathode chamber includes an inlet and an outlet for a cathode chamber solution and an electrode, and a method of electrochemically lysing cells using the same.