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32 条记录 (耗时 0.037 秒)

    Cell lysis method by immobilized metal-ligand complex
    1.
    发明申请
    Cell lysis method by immobilized metal-ligand complex 失效
    固定化金属配体复合物的细胞裂解法

    公开(公告)号：US20060099711A1

    公开(公告)日：2006-05-11

    申请号：US11267005

    申请日：2005-11-04

    申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

    发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

    IPC分类号： G01N1/00

    CPC分类号： C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/308

    摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.

    摘要翻译： 提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。 根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。 此外，由于可以使用各种固体支持体，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

    Cell lysis method by immobilized metal-ligand complex
    2.
    发明授权
    Cell lysis method by immobilized metal-ligand complex 失效
    固定化金属配体复合物的细胞裂解法

    公开(公告)号：US07648838B2

    公开(公告)日：2010-01-19

    申请号：US11267005

    申请日：2005-11-04

    申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

    发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Soo-min Ma ,  Nam Huh ,  Soo-suk Lee

    IPC分类号： G01N33/48 ,  G01N33/20 ,  G01N1/00

    CPC分类号： C12Q1/6806 ,  Y10T436/25 ,  C12Q2523/308

    摘要： Provided is a lysis method for cells or viruses, including: immobilizing a metal-ligand complex on a solid support; and mixing the complex immobilized on the support with a cell or virus solution. According to the lysis method, by immobilizing a chemical on a solid support to perform cell lysis, the dilution problem according to the addition of a cell lysis solution can be resolved and a separate process of removing the chemical is not required so as to reduce the steps upon LOC implementation. In addition, since a variety of solid supports, such as chips, beads, nanoparticles etc. can be used, cell lysis apparatuses of various forms can be fabricated.

    摘要翻译： 提供了一种细胞或病毒的裂解方法，包括：将固定支持物上的金属 - 配体复合物固定; 并将固定在载体上的复合物与细胞或病毒溶液混合。 根据裂解方法，通过将化学物质固定在固体支持体上以进行细胞裂解，可以解决根据细胞溶解溶液的添加的稀释问题，并且不需要单独的除去化学品的方法，以减少 LOC实施步骤。 此外，由于可以使用各种固体支持物，例如芯片，珠粒，纳米粒子等，因此可以制造各种形式的细胞裂解装置。

    Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer
    6.
    发明授权
    Method of isolating and purifying nucleic acids using immobilized hydrogel or PEG-hydrogel copolymer 失效
    使用固定化水凝胶或PEG-水凝胶共聚物分离和纯化核酸的方法

    公开(公告)号：US07803539B2

    公开(公告)日：2010-09-28

    申请号：US11297783

    申请日：2005-12-07

    申请人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

    发明人： Chang-eun Yoo ,  Joon-ho Kim ,  Kyu-youn Hwang ,  Hun-joo Lee ,  Hee-kyun Lim ,  Jun-hong Min

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  C07H21/00

    CPC分类号： C07H21/04

    摘要： Provided is a method of isolating and purifying nucleic acids using an immobilized hydrogel or polyethylene glycol (PEG)-hydrogel copolymer. The method includes: immobilizing a functional group-containing hydrogel or PEG-hydrogel copolymer on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the hydrogel- or PEG-hydrogel copolymer-immobilized substrate to bind the nucleic acids to the hydrogel or the PEG-hydrogel copolymer; washing the nucleic acid-bound hydrogel or PEG-hydrogel copolymer; and eluting the nucleic acids from the hydrogel or the PEG-hydrogel copolymer using an elution solvent. Therefore, binding and elution of nucleic acids can be performed even with no addition of a separate chemical substance, and an effect on a subsequent process such as PCR can be minimized. Furthermore, the amount and intensity for binding nucleic acids can be adjusted according to PEG concentration, and the presence of a hydrogel compound on a substrate enables patterning.

    摘要翻译： 提供了使用固定的水凝胶或聚乙二醇（PEG） - 水凝胶共聚物来分离和纯化核酸的方法。 该方法包括：将含官能团的水凝胶或PEG-水凝胶共聚物固定在基材上; 将含有盐和核酸的混合样品溶液加入到水凝胶或PEG-水凝胶共聚物固定的底物中，以将核酸与水凝胶或PEG-水凝胶共聚物结合; 洗涤核酸结合的水凝胶或PEG-水凝胶共聚物; 并使用洗脱溶剂从水凝胶或PEG-水凝胶共聚物中洗脱核酸。 因此，即使没有添加单独的化学物质也可以进行核酸的结合和洗脱，并且可以最小化对后续过程（例如PCR）的影响。 此外，可以根据PEG浓度调节结合核酸的量和强度，并且在基材上存在水凝胶化合物能够进行图案化。

    Method of controlling pH of electrolyzed solution using microfluidic device including electrolysis device
    10.
    发明授权
    Method of controlling pH of electrolyzed solution using microfluidic device including electrolysis device 有权
    使用包括电解装置的微流体装置控制电解液pH值的方法

    公开(公告)号：US07914660B2

    公开(公告)日：2011-03-29

    申请号：US11303673

    申请日：2005-12-16

    申请人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Kyu-youn Hwang ,  Hee-kyun Lim

    发明人： Hun-joo Lee ,  Joon-ho Kim ,  Chang-eun Yoo ,  Kyu-youn Hwang ,  Hee-kyun Lim

    IPC分类号： C25B1/10

    CPC分类号： G05D21/02 ,  B01D61/422 ,  B01L3/50273 ,  B01L2200/14 ,  B01L2300/0867 ,  B01L2400/0415 ,  C02F1/4618 ,  C02F2103/02 ,  C02F2201/4611 ,  C02F2201/46115 ,  C02F2201/4618 ,  C02F2209/06 ,  Y02E60/366

    摘要： Provided is a method of controlling the pH of a solution using electrolysis in a microfluidic device comprising an electrolysis device including an anode chamber, a cathode chamber, and a partition membrane between the anode chamber and the cathode chamber, wherein the anode chamber includes an inlet and an outlet through which an anode chamber solution enters and is discharged from the anode chamber, respectively, and an electrode, and the cathode chamber includes an inlet and an outlet through which a cathode chamber solution enters and is discharged from the cathode chamber, respectively, and an electrode. The method includes: flowing the anode chamber solution containing a compound having a lower standard oxidation potential than water into the anode chamber through the inlet of the anode chamber; flowing the cathode chamber solution containing a compound having a lower standard reduction potential than water into the cathode chamber through the inlet of the cathode chamber; applying voltage between the electrode in the anode chamber and the electrode in the cathode chamber to induce electrolysis in the anode chamber and the cathode chamber; and mixing equal volumes of the acidic anode-electrolyzed solution and the basic cathode-electrolyzed solution, wherein the volumes of the cathode chamber and the anode chamber are controlled in a predetermined ratio according to a target pH of a solution obtained by mixing the acidic anode-electrolyzed solution and the basic cathode-electrolyzed solution.

    摘要翻译： 提供了一种在微流体装置中使用电解来控制溶液的pH的方法，该微流体装置包括在阳极室和阴极室之间包括阳极室，阴极室和分隔膜的电解装置，其中阳极室包括入口 以及阳极室溶液分别从阳极室进入和排出的出口以及电极，阴极室包括入口和出口，阴极室溶液分别从阴极室溶液进入并从阴极室排出 ，和电极。 该方法包括：将含有比水低的标准氧化电位的化合物的阳极室溶液通过阳极室的入口流入阳极室; 将含有比水低的标准还原电位的化合物的阴极室溶液通过阴极室的入口流入阴极室; 在阳极室中的电极和阴极室中的电极之间施加电压以在阳极室和阴极室中引起电解; 并混合等体积的酸性阳极电解溶液和碱性阴极电解溶液，其中阴极室和阳极室的体积根据通过将酸性阳极混合获得的溶液的目标pH控制在预定比例 电解液和碱性阴极电解溶液。