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公开(公告)号：US20110177556A1
公开(公告)日：2011-07-21
申请号：US13074638
申请日：2011-03-29
CPC分类号： C07K14/70575
摘要： The present invention provides for an isolated polynucleotide sequence encoding a chimeric CD154, comprising a first nucleotide sequence encoding an extracellular subdomain of non-human CD154, preferably murine CD154, that replaces a cleavage site of human CD154, and a second nucleotide sequence encoding an extracellular subdomain of human CD154 that binds to a human CD154 receptor. The present invention also provides for the chimeric CD154 that is encoded by the above-described polynucleotide sequence, an expression vector and a genetic vector comprising the polynucleotide sequence, a host cell comprising the expression vector or the genetic vector, a process for producing the chimeric CD154, and methods for utilizing the expression vectors and genetic constructs containing the chimeric CD154 polynucleotide sequences.
摘要翻译： 本发明提供了编码嵌合CD154的分离的多核苷酸序列，其包含编码非人CD154，优选小鼠CD154的细胞外亚结构域的第一核苷酸序列，其替代人CD154的切割位点，以及编码细胞外的第二核苷酸序列 结合人CD154受体的人CD154的亚结构域。 本发明还提供了由上述多核苷酸序列编码的嵌合CD154，表达载体和包含多核苷酸序列的遗传载体，包含表达载体或遗传载体的宿主细胞， CD154，以及用于使用含有嵌合CD154多核苷酸序列的表达载体和遗传构建体的方法。
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2.发明申请Nucleic Acid Molecules Encoding TNF-Alpha Ligand Polypeptides Having a CD154 Domain 审中-公开编码具有CD154结构域的TNF-α配体多肽的核酸分子公开(公告)号：US20120116052A1
公开(公告)日：2012-05-10
申请号：US13106675
申请日：2011-05-12
CPC分类号： C07K14/525 , A61K38/00 , C07K2319/00
摘要： The present invention is directed to an isolated polynucleotide sequence encoding a chimeric TNFα, comprising a first nucleotide sequence encoding a domain or subdomain of a tumor necrosis factor ligand other than TNFα, wherein the encoded domain or subdomain replaces a cleavage site of native TNFα, and a second nucleotide sequence encoding a domain or subdomain of native TNFα that binds to a TNFα receptor. The encoded chimeric TNFα is significantly less susceptible to cleavage from the cellular surface and, as a result can increase the concentration of a ligand capable of binding to a TNFα receptor on the surface of a cell. The chimeric TNFα is therefore useful in methods for inducing apoptosis of a cell expressing a TNFα receptor, inducing activation of an immune system cell and treating neoplastic cells, by introducing into the cell of interest an isolated polynucleotide sequence encoding a chimeric TNFα that is expressed on the surface of the cell.
摘要翻译： 本发明涉及编码嵌合TNFα的分离的多核苷酸序列，其包含编码除TNFα之外的肿瘤坏死因子配体的结构域或亚结构域的第一核苷酸序列，其中编码的结构域或亚结构域替代天然TNFα的切割位点，以及 编码结合TNFα受体的天然TNFα的结构域或亚结构域的第二核苷酸序列。 编码的嵌合TNFα对细胞表面的切割显着较不敏感，结果可以增加能够结合细胞表面的TNFα受体的配体的浓度。 因此，嵌合TNFα可用于诱导表达TNFα受体的细胞的凋亡，诱导免疫系统细胞的活化和治疗肿瘤细胞的方法，通过将编码嵌合TNFα的分离的多核苷酸序列引入感兴趣的细胞中，所述多核苷酸序列表达于 电池的表面。
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3.发明授权Nucleic acid molecules encoding TNF-α ligand polypeptides having a CD154 domain 有权编码具有CD154结构域的TNF-α配体多肽的核酸分子公开(公告)号：US07786282B2
公开(公告)日：2010-08-31
申请号：US10006305
申请日：2001-12-06
CPC分类号： C07K14/525 , A61K38/00 , C07K2319/00
摘要： The present invention is directed to an isolated polynucleotide sequence encoding a chimeric TNFα and chimeric TNFα polypeptides. The former have a first nucleotide sequence encoding a domain or subdomain of a tumor necrosis factor ligand other than TNFα, wherein the encoded domain or subdomain lacks a cleavage site, and a second nucleotide sequence encoding a domain or subdomain of native TNFα that binds to a TNFα receptor. The encoded chimeric TNFα is significantly less susceptible to cleavage from the cellular surface and, as a result can increase the concentration of a ligand capable of binding to a TNFα receptor on the surface of a cell. The chimeric TNFα is therefore useful in methods for inducing apoptosis of a cell expressing a TNFα receptor, inducing activation of an immune system cell and treating neoplastic cells, by introducing into the cell of interest an isolated polynucleotide sequence encoding a chimeric TNFα that is expressed on the surface of the cell.
摘要翻译： 本发明涉及编码嵌合TNFα和嵌合TNFα多肽的分离的多核苷酸序列。 前者具有编码除TNFα之外的肿瘤坏死因子配体的结构域或亚结构域的第一核苷酸序列，其中编码的结构域或亚结构域缺乏切割位点，以及编码天然TNFα结构域或亚结构域的第二核苷酸序列 TNFα受体。 编码的嵌合TNFα对细胞表面的切割显着较不敏感，结果可以增加能够结合细胞表面的TNFα受体的配体的浓度。 因此，嵌合TNFα可用于诱导表达TNFα受体的细胞的凋亡，诱导免疫系统细胞的活化和治疗肿瘤细胞的方法，通过将编码嵌合TNFα的分离的多核苷酸序列引入感兴趣的细胞中，所述多核苷酸序列表达于 电池的表面。
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4.发明授权Chimeric nucleic acids encoding polypeptides comprising TNF alpha, CD70 and FAS ligand domains 有权编码包含TNFα，CD70和FAS配体结构域的多肽的嵌合核酸公开(公告)号：US07964712B2
公开(公告)日：2011-06-21
申请号：US12871807
申请日：2010-08-30
CPC分类号： C07K14/525 , A61K38/00 , C07K2319/00
摘要： The present invention is directed to an isolated polynucleotide sequence encoding a chimeric TNFα, comprising a first nucleotide sequence encoding a domain or subdomain of a tumor necrosis factor ligand other than TNFα, wherein the encoded domain or subdomain replaces a cleavage site of native TNFα, and a second nucleotide sequence encoding a domain or subdomain of native TNFα that binds to a TNFα receptor. The encoded chimeric TNFα is significantly less susceptible to cleavage from the cellular surface and, as a result can increase the concentration of a ligand capable of binding to a TNFα receptor on the surface of a cell. The chimeric TNFα is therefore useful in methods for inducing apoptosis of a cell expressing a TNFα receptor, inducing activation of an immune system cell and treating neoplastic cells, by introducing into the cell of interest an isolated polynucleotide sequence encoding a chimeric TNFα that is expressed on the surface of the cell.
摘要翻译： 本发明涉及编码嵌合TNFα的分离的多核苷酸序列，其包含编码除TNFα之外的肿瘤坏死因子配体的结构域或亚结构域的第一核苷酸序列，其中编码的结构域或亚结构域替代天然TNFα的切割位点，以及 编码结合TNFα受体的天然TNFα的结构域或亚结构域的第二核苷酸序列。 编码的嵌合TNFα对细胞表面的切割显着较不敏感，结果可以增加能够结合细胞表面的TNFα受体的配体的浓度。 因此，嵌合TNFα可用于诱导表达TNFα受体的细胞的凋亡，诱导免疫系统细胞的活化和治疗肿瘤细胞的方法，通过将编码嵌合TNFα的分离的多核苷酸序列引入感兴趣的细胞中，所述多核苷酸序列表达于 电池的表面。
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公开(公告)号：US07928213B2
公开(公告)日：2011-04-19
申请号：US12389904
申请日：2009-02-20
CPC分类号： C07K14/70575
摘要： The present invention provides for an isolated polynucleotide sequence encoding a chimeric CD154, comprising a first nucleotide sequence encoding an extracellular subdomain of non-human CD154, preferably murine CD154, that replaces a cleavage site of human CD154, and a second nucleotide sequence encoding an extracellular subdomain of human CD154 that binds to a human CD154 receptor. The present invention also provides for the chimeric CD154 that is encoded by the above-described polynucleotide sequence, an expression vector and a genetic vector comprising the polynucleotide sequence, a host cell comprising the expression vector or the genetic vector, a process for producing the chimeric CD154, and methods for utilizing the expression vectors and genetic constructs containing the chimeric CD154 polynucleotide sequences.
摘要翻译： 本发明提供了编码嵌合CD154的分离的多核苷酸序列，其包含编码非人CD154，优选小鼠CD154的细胞外亚结构域的第一核苷酸序列，其替代人CD154的切割位点，以及编码细胞外的第二核苷酸序列 结合人CD154受体的人CD154的亚结构域。 本发明还提供了由上述多核苷酸序列编码的嵌合CD154，表达载体和包含多核苷酸序列的遗传载体，包含表达载体或遗传载体的宿主细胞， CD154，以及用于使用含有嵌合CD154多核苷酸序列的表达载体和遗传构建体的方法。
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公开(公告)号：US20110008842A1
公开(公告)日：2011-01-13
申请号：US12871807
申请日：2010-08-30
IPC分类号： C12P21/02 , C07H21/00 , C12N15/63 , C12N15/86 , C12N15/74 , C12N15/861 , C12N15/867 , C12N5/10 , C07K14/00
CPC分类号： C07K14/525 , A61K38/00 , C07K2319/00
摘要： The present invention is directed to an isolated polynucleotide sequence encoding a chimeric TNFα, comprising a first nucleotide sequence encoding a domain or subdomain of a tumor necrosis factor ligand other than TNFα, wherein the encoded domain or subdomain replaces a cleavage site of native TNFα, and a second nucleotide sequence encoding a domain or subdomain of native TNFα that binds to a TNFα receptor. The encoded chimeric TNFα is significantly less susceptible to cleavage from the cellular surface and, as a result can increase the concentration of a ligand capable of binding to a TNFα receptor on the surface of a cell. The chimeric TNFα is therefore useful in methods for inducing apoptosis of a cell expressing a TNFα receptor, inducing activation of an immune system cell and treating neoplastic cells, by introducing into the cell of interest an isolated polynucleotide sequence encoding a chimeric TNFα that is expressed on the surface of the cell.
摘要翻译： 本发明涉及编码嵌合TNFα的分离的多核苷酸序列，其包含编码除TNFα之外的肿瘤坏死因子配体的结构域或亚结构域的第一核苷酸序列，其中编码的结构域或亚结构域替代天然TNFα的切割位点，以及 编码结合TNFα受体的天然TNFα的结构域或亚结构域的第二核苷酸序列。 编码的嵌合TNFα对细胞表面的切割显着较不敏感，结果可以增加能够结合细胞表面的TNFα受体的配体的浓度。 因此，嵌合TNFα可用于诱导表达TNFα受体的细胞的凋亡，诱导免疫系统细胞的活化和治疗肿瘤细胞的方法，通过将编码嵌合TNFα的分离的多核苷酸序列引入感兴趣的细胞中，所述多核苷酸序列表达于 电池的表面。
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公开(公告)号：US08138310B2
公开(公告)日：2012-03-20
申请号：US13074638
申请日：2011-03-29
IPC分类号： C07K14/705
CPC分类号： C07K14/70575
摘要： The present invention provides for an isolated polynucleotide sequence encoding a chimeric CD154, comprising a first nucleotide sequence encoding an extracellular subdomain of non-human CD154, preferably murine CD154, that replaces a cleavage site of human CD154, and a second nucleotide sequence encoding an extracellular subdomain of human CD154 that binds to a human CD154 receptor. The present invention also provides for the chimeric CD154 that is encoded by the above-described polynucleotide sequence, an expression vector and a genetic vector comprising the polynucleotide sequence, a host cell comprising the expression vector or the genetic vector, a process for producing the chimeric CD154, and methods for utilizing the expression vectors and genetic constructs containing the chimeric CD154 polynucleotide sequences.
摘要翻译： 本发明提供了编码嵌合CD154的分离的多核苷酸序列，其包含编码非人CD154，优选小鼠CD154的细胞外亚结构域的第一核苷酸序列，其替代人CD154的切割位点，以及编码细胞外的第二核苷酸序列 结合人CD154受体的人CD154的亚结构域。 本发明还提供了由上述多核苷酸序列编码的嵌合CD154，表达载体和包含多核苷酸序列的遗传载体，包含表达载体或遗传载体的宿主细胞， CD154，以及用于使用含有嵌合CD154多核苷酸序列的表达载体和遗传构建体的方法。
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公开(公告)号：US07495090B2
公开(公告)日：2009-02-24
申请号：US10154759
申请日：2002-05-23
CPC分类号： C07K14/70575
摘要： The present invention provides for an isolated polynucleotide sequence encoding a chimeric CD154, comprising a first nucleotide sequence encoding an extracellular subdomain of non-human CD154, preferably murine CD154, that replaces a cleavage site of human CD154, and a second nucleotide sequence encoding an extracellular subdomain of human CD154 that binds to a human CD154 receptor. The present invention also provides for the chimeric CD154 that is encoded by the above-described polynucleotide sequence, an expression vector and a genetic vector comprising the polynucleotide sequence, a host cell comprising the expression vector or the genetic vector, a process for producing the chimeric CD154, and methods for utilizing the expression vectors and genetic constructs containing the chimeric CD154 polynucleotide sequences.
摘要翻译： 本发明提供了编码嵌合CD154的分离的多核苷酸序列，其包含编码非人CD154，优选小鼠CD154的细胞外亚结构域的第一核苷酸序列，其替代人CD154的切割位点，以及编码细胞外的第二核苷酸序列 结合人CD154受体的人CD154的亚结构域。 本发明还提供了由上述多核苷酸序列编码的嵌合CD154，表达载体和包含多核苷酸序列的遗传载体，包含表达载体或遗传载体的宿主细胞， CD154，以及用于使用含有嵌合CD154多核苷酸序列的表达载体和遗传构建体的方法。
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公开(公告)号：US09250233B2
公开(公告)日：2016-02-02
申请号：US12934624
申请日：2009-03-27
IPC分类号： G01N33/543 , G01N33/537 , G01N33/68 , G01N33/94
CPC分类号： G01N33/6854 , G01N33/537 , G01N33/54326 , G01N33/577 , G01N33/582 , G01N33/94
摘要： Methods for detection of any antibody utilizing a standardized approach applicable to any antibody which provides highly specific assays specific for individual or multiple antibodies. The methods enable improved pharmacokinetic analysis during development and clinical use of antibody-based therapies as well as determination of diagnostic and/or prognostic factors.
摘要翻译： 使用适用于提供对个体或多个抗体特异性高度特异性测定的任何抗体的标准化方法来检测任何抗体的方法。 该方法能够改进基于抗体的疗法的开发和临床应用中的药代动力学分析以及诊断和/或预后因素的确定。
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10.发明授权公开(公告)号：US09217040B2
公开(公告)日：2015-12-22
申请号：US13997934
申请日：2012-01-13
申请人： Thomas J. Kipps , George F. Widhopf, II , Bing Cui
发明人： Thomas J. Kipps , George F. Widhopf, II , Bing Cui
CPC分类号： G01N33/6854 , A61K2039/505 , C07K16/2803 , C07K16/40 , C07K2317/565 , C07K2317/73 , C07K2317/77 , C07K2317/92
摘要： Therapeutic antibodies having binding specificity for ROR-1 expressed on cancer cells (particularly leukemic and lymphomic cells) and pharmaceutical compositions containing one or more such antibodies for use in treating cancer. Methods for diagnosing such cancers through in vitro detection of binding to ROR-1 protein expressed on putative cancer cells are also provided.
摘要翻译： 在癌细胞（特别是白血病和淋巴细胞）上表达的对ROR-1具有结合特异性的治疗性抗体以及含有一种或多种用于治疗癌症的抗体的药物组合物。 还提供了通过体外检测与推定的癌细胞上表达的ROR-1蛋白的结合来诊断这种癌症的方法。
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