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1.发明授权公开(公告)号：US07138041B2
公开(公告)日：2006-11-21
申请号：US10784681
申请日：2004-02-23
IPC分类号： G01N27/327
CPC分类号： G01N27/3272
摘要： An electrochemical biosensor formed by screen printing and method of fabricating such biosensor is disclosed in the present invention. The biosensor can quickly absorb a sample to be measured therein, effectively control volume of the sample fed and “fill-and-position” the sample therein. The biosensor includes an electrode layer (electrode area) comprising two or three electrodes, which are a working electrode, a reference electrode and an auxiliary electrode (tri-electrode) on an insulating substrate. An active reaction layer containing reactant, reaction catalyst, mediator, wetting agent and surfactant is spread on the surface of the electrode layer. A sample inflow area is formed above the electrode area by adding an upper cover on top of a middle insulating layer with a U-shaped opening formed therein. Sample solution with a minute amount about 0.8 to 1 μl can be rapidly introduced into the electrode area and the active reaction layer via the inflow area by siphon or capillary, where the ingredient of the sample can be analysed by measuring reaction between the sample, reaction catalyst and mediator in the reaction layer using electrochemical potentiometric or amperometric method. An upwardly extended closed space formed within the upper cover above the electrode area adjacent to the front of conductive wires can be effectively used to control sample volume and “fill-and-position” the sample.
摘要翻译： 在本发明中公开了通过丝网印刷形成的电化学生物传感器和制造这种生物传感器的方法。 生物传感器可以快速吸收要测量的样品，有效地控制样品进料的体积并将样品“填充并定位”。 生物传感器包括在绝缘基板上包括作为工作电极，参考电极和辅助电极（三电极）的两个或三个电极的电极层（电极区域）。 含有反应物，反应催化剂，介体，润湿剂和表面活性剂的活性反应层扩散在电极层的表面上。 通过在其中形成有U形开口的中间绝缘层的顶部添加上盖，在电极区域的上方形成样品流入区域。 通过虹吸或毛细管可以通过虹吸或毛细管通过流入区域快速地将约0.8至1微摩尔的微量的样品溶液引入电极区域和活性反应层，其中样品的成分可以通过测量样品，反应 催化剂和介质在反应层中使用电化学电位或电流法。 可以有效地使用形成在电极区域之上的上电极区域内的向上延伸的封闭空间，该电极区域与导电线的前部相邻，以控制样品体积和“填充和定位”样品。
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公开(公告)号：US20080160543A1
公开(公告)日：2008-07-03
申请号：US11645556
申请日：2006-12-27
申请人： Chein-Shyong Su , Wen-Jing Cheng , Maio-Ling Hung , Tai-Guang Wu
发明人： Chein-Shyong Su , Wen-Jing Cheng , Maio-Ling Hung , Tai-Guang Wu
IPC分类号： G01N33/53
CPC分类号： C12Q1/001 , G01N33/5438 , G01N33/54386
摘要： An electrochemical immuno diagnostic strip and its preparation method are disclosed herein. A screen-printed electrode is prepared on an insulating substrate, which is divided into an electrode area and a connecting area. An electrochemical precursor is placed on the electrode area and a reaction membrane is provided on the electrode area. A specimen loading area is at the reaction membrane. The present invention is advantageous to overcome the interference problem of the electrochemical detection technology and to provide the quantitative analysis of the prompt diagnostic strip.
摘要翻译： 本文公开了一种电化学免疫诊断条及其制备方法。 在绝缘基板上制备丝网印刷电极，该绝缘基板被分成电极区域和连接区域。 在电极区域上放置电化学前体，在电极区域上设置反应膜。 样品装载区域在反应膜上。 本发明有利于克服电化学检测技术的干扰问题，并提供快速诊断条的定量分析。
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3.发明申请Electrochemical method for detecting hemoglobin or hematocrit and test strip thereof 有权用于检测血红蛋白或血细胞比容及其测试条的电化学方法公开(公告)号：US20090104641A1
公开(公告)日：2009-04-23
申请号：US12003564
申请日：2007-12-28
申请人： Chein-Shyong Su , Tai-Guang Wu
发明人： Chein-Shyong Su , Tai-Guang Wu
CPC分类号： G01N33/726
摘要： The present invention provides an electrochemical method and a test strip for detecting hemoglobin in a specimen. The method includes the steps of providing the specimen with a reagent, which has a buffer solution, a surfactant and an electron mediator, tetrathiafulvalene, modified by cyclodextrin; detecting electric current produced by the reaction of the hemoglobin and the electron mediator in a specimen under a potentiostatic condition; and calculating a hemoglobin concentration in the specimen according to the detected electric current. In the method of the present invention, tetrathiafulvalene modified by cyclodextrin is used as an electron mediator, which directly interacts with the hemoglobin in the specimen. Since the hemoglobin concentration is in inverse proportion to the value of electric current or the value of SOC (state-of-charge) integrated by electric current value with time, the hemoglobin concentration in a whole blood specimen or the hematocrit level in a blood specimen is accurately and rapidly obtained according to the detected electric value or the value of SOC.
摘要翻译： 本发明提供一种用于检测样品中的血红蛋白的电化学方法和测试条。 该方法包括以下步骤：向样品提供具有缓冲溶液，表面活性剂和电子介体的试剂，由环糊精改性的四硫富瓦烯; 在恒电位条件下检测样品中血红蛋白和电子介体的反应产生的电流; 并根据检测到的电流计算样品中的血红蛋白浓度。 在本发明的方法中，将通过环糊精改性的四硫富瓦烯用作电子介体，其直接与样品中的血红蛋白相互作用。 由于血红蛋白浓度与电流值或通过电流值随时间积分的SOC（充电状态）的值成反比，所以血样中的血红蛋白浓度或血样中的血细胞比容水平 根据检测到的电值或SOC的值准确而快速地获得。
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公开(公告)号：US07749764B2
公开(公告)日：2010-07-06
申请号：US12003564
申请日：2007-12-28
申请人： Chein-Shyong Su , Tai-Guang Wu
发明人： Chein-Shyong Su , Tai-Guang Wu
IPC分类号： G01N33/72
CPC分类号： G01N33/726
摘要： An electrochemical method and a test strip for detecting hemoglobin in a specimen are provided. The method includes the steps of providing the specimen with a reagent including a buffer solution, a surfactant and an electron mediator, tetrathiafulvalene, modified by cyclodextrin; detecting electric current produced by reaction of the hemoglobin and the electron mediator in the specimen under a potentiostatic condition; and calculating a concentration of the hemoglobin in the specimen according to the detected electric current.
摘要翻译： 提供了用于检测试样中的血红蛋白的电化学方法和测试条。 该方法包括以下步骤：向试样提供包含缓冲溶液，表面活性剂和电子介体的试剂，由环糊精改性的四硫富瓦烯; 在恒电位条件下检测样品中血红蛋白和电子介体反应产生的电流; 以及根据检测到的电流计算样品中血红蛋白的浓度。
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公开(公告)号：US20080057525A1
公开(公告)日：2008-03-06
申请号：US11515881
申请日：2006-09-06
申请人： Chein-Shyong Su , Hung Miao-Ling , Ning-Jun Jing , Tai-Guang Wu
发明人： Chein-Shyong Su , Hung Miao-Ling , Ning-Jun Jing , Tai-Guang Wu
IPC分类号： C12Q1/60
摘要： A sensing member for detecting total cholesterol of a blood sample is disclosed, which includes: a working electrode and a counter electrode respectively formed on a first substrate and a second substrate, wherein, an opening is formed in one of the first and second substrates corresponding in position to the electrode for allowing the blood sample to pass through; a dielectric layer formed on one of the surfaces of the first and second substrates and exposing the electrode and end connector such that the end connector can be electrically connected to an electronic device used for applying electric voltage; a reactive pad disposed between the working electrode and the counter electrode and containing an reactive reagent, wherein the reactive reagent comprises an electron transport material, an enzyme capable of reacting with the analyte and generating a current corresponding to the concentration, and a buffer solution; and a separating pad disposed on the opening for removing interfering materials from the blood sample before the blood sample enters into the opening. By disposing the separating pad at a specified position, corpuscles and interfering giant molecules can be removed from the blood sample so as to quickly and accurately detect total cholesterol of the whole blood sample.
摘要翻译： 公开了一种用于检测血液样本的总胆固醇的感测部件，其包括：分别形成在第一基板和第二基板上的工作电极和对置电极，其中，开口形成在第一和第二基板中的一个对应物 位于电极上以允许血液样品通过; 形成在所述第一和第二基板的一个表面上的电介质层，并且暴露所述电极和端部连接器，使得所述端部连接器可以电连接到用于施加电压的电子装置; 设置在工作电极和对电极之间并含有反应性试剂的反应性焊盘，其中反应试剂包括电子传输材料，能够与分析物反应并产生对应于浓度的电流的酶和缓冲溶液; 以及设置在开口上的分离垫，用于在血液样本进入开口之前从血液样品中除去干扰物质。 通过将分离垫设置在指定位置，可以从血液样品中除去小体和干扰性的巨型分子，以便快速准确地检测全血样品的总胆固醇。
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6.发明申请公开(公告)号：US20050183953A1
公开(公告)日：2005-08-25
申请号：US10784681
申请日：2004-02-23
IPC分类号： G01N27/26 , G01N33/487
CPC分类号： G01N27/3272
摘要： An electrochemical biosensor formed by screen printing and method of fabricating such biosensor is disclosed in the present invention. The biosensor can quickly absorb a sample to be measured therein, effectively control volume of the sample fed and “fill-and-position” the sample therein. The biosensor includes an electrode layer (electrode area) comprising two or three electrodes, which are a working electrode, a reference electrode and an auxiliary electrode (tri-electrode) on an insulating substrate. An active reaction layer containing reactant, reaction catalyst, mediator, wetting agent and surfactant is spread on the surface of the electrode layer. A sample inflow area is formed above the electrode area by adding an upper cover on top of a middle insulating layer with a U-shaped opening formed therein. Sample solution with a minute amount about 0.8 to 1 μl can be rapidly introduced into the electrode area and the active reaction layer via the inflow area by siphon or capillary, where the ingredient of the sample can be analysed by measuring reaction between the sample, reaction catalyst and mediator in the reaction layer using electrochemical potentiometric or amperometric method. An upwardly extended closed space formed within the upper cover above the electrode area adjacent to the front of conductive wires can be effectively used to control sample volume and “fill-and-position” the sample.
摘要翻译： 在本发明中公开了通过丝网印刷形成的电化学生物传感器和制造这种生物传感器的方法。 生物传感器可以快速吸收要测量的样品，有效地控制样品进料的体积并将样品“填充并定位”。 生物传感器包括在绝缘基板上包括作为工作电极，参考电极和辅助电极（三电极）的两个或三个电极的电极层（电极区域）。 含有反应物，反应催化剂，介体，润湿剂和表面活性剂的活性反应层扩散在电极层的表面上。 通过在其中形成有U形开口的中间绝缘层的顶部添加上盖，在电极区域的上方形成样品流入区域。 通过虹吸或毛细管可以通过虹吸或毛细管通过流入区域快速地将约0.8至1微摩尔的微量的样品溶液引入电极区域和活性反应层，其中样品的成分可以通过测量样品，反应 催化剂和介质在反应层中使用电化学电位或电流法。 可以有效地使用形成在电极区域之上的上电极区域内的向上延伸的封闭空间，该电极区域与导电线的前部相邻，以控制样品体积和“填充和定位”样品。
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7.发明授权Interference-eliminating membranes, test strips, kits and methods for use in uric acid assay 有权干扰消除膜，测试条，用于尿酸测定的试剂盒和方法公开(公告)号：US06699720B1
公开(公告)日：2004-03-02
申请号：US09579430
申请日：2000-05-26
申请人： Tsai Yun Lee , Yi Chih Lei , Shi-Yuan Sheu , Yu Fen Tsai , Tai-Guang Wu , Hung-Hsiu Ho , Ming-Yen Kuo , Jiuan J. Liu , Tong H. Chang , Hsueh-Fang Chen , Liahng-Yirn Liu
发明人： Tsai Yun Lee , Yi Chih Lei , Shi-Yuan Sheu , Yu Fen Tsai , Tai-Guang Wu , Hung-Hsiu Ho , Ming-Yen Kuo , Jiuan J. Liu , Tong H. Chang , Hsueh-Fang Chen , Liahng-Yirn Liu
IPC分类号： G01N2178
CPC分类号： G01N33/52 , Y10T436/14 , Y10T436/148888 , Y10T436/25 , Y10T436/25125
摘要： The present invention relates to an interference-eliminating membrane for use in detecting uric acid in a sample, comprising a compound for inhibiting or shading uric acid interfering substances, or derivatives thereof, and a carrier having an absorption property and permeability for the sample; and a process for preparing the interference-eliminating membrane. The present invention also provides a test strip for use in detecting uric acid in a sample, comprising a reagent reaction layer or optional interference-eliminating membranes and/or support layers; and a kit comprising the test strip of the invention.
摘要翻译： 本发明涉及一种用于检测样品中尿酸的干扰消除膜，其包含用于抑制或遮蔽尿酸干扰物质的化合物或其衍生物，以及具有该样品的吸收性和渗透性的载体。 本发明还提供一种用于检测样品中的尿酸的测试条，包括试剂反应层或任选的干扰消除膜和/或支持层; 以及包括本发明的测试条的试剂盒。
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