公开(公告)号：US20090263328A1

公开(公告)日：2009-10-22

申请号：US12424069

申请日：2009-04-15

申请人： Chika NAKAJIMA ,  Yoshihisa TANIKAWA ,  Nobuyuki NAGASAWA

发明人： Chika NAKAJIMA ,  Yoshihisa TANIKAWA ,  Nobuyuki NAGASAWA

IPC分类号： A61K49/00 ,  C12M1/34 ,  A61P35/00

CPC分类号： G01N21/6456

摘要： A site of interest to be observed is clearly observed in an image during fluoroscopy of a small laboratory animal, even when the fluorescence is extremely low. The invention provides a fluorescence observation apparatus including a light source that emits excitation light; an optical system that irradiates an image-acquisition site on a small laboratory animal with the excitation light from the light source; a light-blocking unit that blocks light in a prescribed region of the small laboratory animal or in an image of the prescribed region; an image-acquisition unit that acquires a fluorescence image of the small laboratory animal; and a control unit configured to identify a high-fluorescence region having a prescribed fluorescence level or above in the fluorescence image of the small laboratory animal acquired by the image-acquisition unit and to control the light-blocking unit so as to block light at the identified high-fluorescence region.

摘要翻译： 即使荧光极低，在小型实验室动物的荧光检查中，也可以在图像中观察到需要观察的位置。 本发明提供了一种荧光观察装置，其包括发射激发光的光源; 光学系统，其利用来自光源的激发光照射小实验动物的图像采集部位; 遮光单元，其阻挡小实验室动物的规定区域或规定区域的图像中的光; 获取小型实验室动物的荧光图像的图像获取单元; 以及控制单元，被配置为在由所述图像获取单元获取的所述小型实验动物的荧光图像中识别具有规定的荧光水平以上的高荧光区域，并且控制所述遮光单元以阻挡所述 鉴定出高荧光区。

公开(公告)号：US20090140129A1

公开(公告)日：2009-06-04

申请号：US12326419

申请日：2008-12-02

申请人： Yasuaki NATORI ,  Nobuyuki NAGASAWA

发明人： Yasuaki NATORI ,  Nobuyuki NAGASAWA

IPC分类号： G01J1/04

CPC分类号： G02B21/16 ,  G02B21/24

摘要： To prevent incidence of strong external light into a photodetector and an imaging device while a lid is opened, so as to thereby perform accurate photodetection and imaging operations without receiving the influence of external light. An observation apparatus comprising: a stage on which a sample serving as an observation target is placed; a detection unit which detects light from the sample; an imaging optical system which projects the light from the sample onto the detection unit; a light shielding member which covers the whole or a part thereof; an opening provided in the light shielding member; a lid which opens/closes the opening; an open/close detection unit which detects the open/close state of the lid; a dimmer which limits light incident into the detection unit; and a controller which operates the dimmer to reduce the amount of the light incident into the detection unit, when the open/close detection unit detects that the lid has been opened.

摘要翻译： 为了防止在打开盖子时将强烈的外部光入射到光电检测器和成像装置中，从而在不受到外部光的影响的情况下进行精确的光电检测和成像操作。 一种观察装置，包括：放置有作为观察对象的样本的阶段; 检测来自样品的光的检测单元; 成像光学系统，其将来自样品的光投射到检测单元上; 覆盖其全部或一部分的遮光构件; 设在遮光部件上的开口部; 打开/关闭开口的盖子; 检测盖的打开/关闭状态的打开/关闭检测单元; 限制入射到检测单元中的光的调光器; 以及当打开/关闭检测单元检测到盖已经打开时，操作调光器以减少入射到检测单元中的光量的控制器。

公开(公告)号：US20120082796A1

公开(公告)日：2012-04-05

申请号：US13252452

申请日：2011-10-04

申请人： Tomohito SHIMIZU ,  Shuji HANAI ,  Hideyuki KOBORI ,  Nobuyuki NAGASAWA ,  Tetsuya HARA

发明人： Tomohito SHIMIZU ,  Shuji HANAI ,  Hideyuki KOBORI ,  Nobuyuki NAGASAWA ,  Tetsuya HARA

IPC分类号： B05D1/30 ,  B05C5/00

CPC分类号： B05D1/305 ,  B05C5/005 ,  B05C5/008 ,  B05D7/56 ,  B05D2252/02

摘要： A curtain coating method including: ejecting at least one layer of a coating liquid from a slit, and making the ejected coating liquid fall freely from a curtain nozzle lip by using a curtain edge guide which guides the coating liquid in the form of a curtain liquid film, so as to apply the coating liquid onto a continuously running web, wherein the coating liquid is applied by moving the curtain edge guide toward the depth direction when the curtain liquid film is seen from the front.

摘要翻译： 一种帘式涂布方法，包括：从狭缝喷射至少一层涂布液，并且通过使用将幕涂液体形式引导的帘式边缘引导件使喷出的涂布液从帘式喷嘴唇部自由落下 膜，以便将涂布液施加到连续流动的纸幅上，其中当从前面观看帘式液体膜时，通过使帘式边缘引导件向深度方向移动而施加涂布液。

公开(公告)号：US20090161929A1

公开(公告)日：2009-06-25

申请号：US12332540

申请日：2008-12-11

申请人： Masahiro OBA ,  Nobuyuki NAGASAWA

发明人： Masahiro OBA ,  Nobuyuki NAGASAWA

IPC分类号： G06K9/00

CPC分类号： G06K9/20 ,  G06T5/50 ,  G06T7/0012 ,  G06T2207/20221 ,  H04N5/23232 ,  H04N5/2352

摘要： When a biological specimen, such as living cells, biological tissue, or a small animal, is quantitatively observed, the specimen is observed for a long time or over a long period, while maintaining the quantitative properties. Provided is an observation method of observing luminescence or fluorescence emitted from a biological specimen. The observation method includes a referring step of referring to image data previously acquired; an intensity determining step of determining whether the intensity of the image data previously acquired is saturated; an exposure dividing step of dividing the exposure of the image data previously acquired one time into a plurality of exposures when the intensity is determined as being saturated in the intensity determining step; an image acquiring step of acquiring images the same number of times as the number of divided exposures; and an integrating step of integrating the images separately acquired multiple times into a single image.

摘要翻译： 当定量观察诸如活细胞，生物组织或小动物的生物样本时，长时间或长时间观察样本，同时保持定量性质。 提供了观察从生物样本发出的发光或荧光的观察方法。 观察方法包括参照先前获取的图像数据的参考步骤; 强度确定步骤，确定先前获取的图像数据的强度是否饱和; 曝光分割步骤，当在所述强度确定步骤中确定所述强度被确定为饱和时，将先前获取的一次的图像数据的曝光除以多个曝光; 图像获取步骤，获取与分割曝光数相同次数的图像; 以及将多次分开获取的图像集成到单个图像中的整合步骤。