公开(公告)号：US08647719B2

公开(公告)日：2014-02-11

申请号：US13252452

申请日：2011-10-04

Applicant: Tomohito Shimizu ,  Shuji Hanai ,  Hideyuki Kobori ,  Nobuyuki Nagasawa ,  Tetsuya Hara

Inventor： Tomohito Shimizu ,  Shuji Hanai ,  Hideyuki Kobori ,  Nobuyuki Nagasawa ,  Tetsuya Hara

IPC: B05D1/30

CPC classification number: B05D1/305 ,  B05C5/005 ,  B05C5/008 ,  B05D7/56 ,  B05D2252/02

Abstract: A curtain coating method including: ejecting at least one layer of a coating liquid from a slit, and making the ejected coating liquid fall freely from a curtain nozzle lip by using a curtain edge guide which guides the coating liquid in the form of a curtain liquid film, so as to apply the coating liquid onto a continuously running web, wherein the coating liquid is applied by moving the curtain edge guide toward the depth direction when the curtain liquid film is seen from the front.

Abstract translation: 一种帘式涂布方法，包括：从狭缝喷射至少一层涂布液，并且通过使用将幕涂液体形式引导的帘式边缘引导件使喷出的涂布液从帘式喷嘴唇部自由落下 膜，以便将涂布液施加到连续流动的纸幅上，其中当从前面观察帘式液体膜时，通过使帘式边缘引导件向深度方向移动而施加涂布液。

公开(公告)号：US20090140129A1

公开(公告)日：2009-06-04

申请号：US12326419

申请日：2008-12-02

Applicant: Yasuaki NATORI ,  Nobuyuki NAGASAWA

Inventor： Yasuaki NATORI ,  Nobuyuki NAGASAWA

IPC: G01J1/04

CPC classification number: G02B21/16 ,  G02B21/24

Abstract: To prevent incidence of strong external light into a photodetector and an imaging device while a lid is opened, so as to thereby perform accurate photodetection and imaging operations without receiving the influence of external light. An observation apparatus comprising: a stage on which a sample serving as an observation target is placed; a detection unit which detects light from the sample; an imaging optical system which projects the light from the sample onto the detection unit; a light shielding member which covers the whole or a part thereof; an opening provided in the light shielding member; a lid which opens/closes the opening; an open/close detection unit which detects the open/close state of the lid; a dimmer which limits light incident into the detection unit; and a controller which operates the dimmer to reduce the amount of the light incident into the detection unit, when the open/close detection unit detects that the lid has been opened.

Abstract translation: 为了防止在打开盖子时将强烈的外部光入射到光电检测器和成像装置中，从而在不受到外部光的影响的情况下进行精确的光电检测和成像操作。 一种观察装置，包括：放置有作为观察对象的样本的阶段; 检测来自样品的光的检测单元; 成像光学系统，其将来自样品的光投射到检测单元上; 覆盖其全部或一部分的遮光构件; 设在遮光部件上的开口部; 打开/关闭开口的盖子; 检测盖的打开/关闭状态的打开/关闭检测单元; 限制入射到检测单元中的光的调光器; 以及当打开/关闭检测单元检测到盖已经打开时，操作调光器以减少入射到检测单元中的光量的控制器。

公开(公告)号：US07436562B2

公开(公告)日：2008-10-14

申请号：US11375042

申请日：2006-03-15

Applicant: Nobuyuki Nagasawa ,  Yoshihisa Tanikawa ,  Kazuhiko Osa ,  Yoshiharu Saito

Inventor： Nobuyuki Nagasawa ,  Yoshihisa Tanikawa ,  Kazuhiko Osa ,  Yoshiharu Saito

IPC: G02B26/08 ,  G02B21/06

CPC classification number: G02B21/006 ,  A61B5/0059 ,  A61B5/0068 ,  G02B21/0012 ,  G02B21/0028 ,  G02B26/0816

Abstract: It is possible to easily and accurately confirm the position of an optical axis of an objective lens relative to an examination site on a specimen, and positioning of the objective relative to the specimen can be carried out rapidly in a preparation stage. The invention provides scanning examination apparatus comprising a first light source; a light scanning unit configured to scan light from the first light source on a specimen; an objective lens configured to form an image of the light scanned in the light scanning unit at the specimen; a light-detecting unit configured to detect return light emitted from the specimen; a second light source configured to emit visible light; a deflecting optical element, disposed between the light scanning unit and the objective lens, for making visible light emitted from the second light source enter the objective lens along an optical axis of the objective lens; and a beam-shaping unit configured to form the visible light from the second light source, which is irradiated onto a surface of the specimen via the objective lens using the deflecting optical element, into a pattern that enables the optical axis of the objective lens to be indicated.

Abstract translation: 可以容易且准确地确认物镜的光轴相对于检体的检查部位的位置，能够在准备阶段快速地进行物体相对于检体的定位。 本发明提供一种扫描检查装置，包括：第一光源; 光扫描单元，被配置为在样本上扫描来自第一光源的光; 物镜，被配置为在所述样本处形成在所述光扫描单元中扫描的光的图像; 光检测单元，被配置为检测从所述样本发射的返回光; 配置为发射可见光的第二光源; 设置在光扫描单元和物镜之间的偏转光学元件，用于使从第二光源发出的可见光沿着物镜的光轴进入物镜; 以及光束整形单元，被配置为将来自所述第二光源的可见光通过所述偏转光学元件通过所述物镜照射到所述样本的表面上，使得能够使所述物镜的光轴 被指示。

公开(公告)号：US20080116395A1

公开(公告)日：2008-05-22

申请号：US12005893

申请日：2007-12-27

Applicant: Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

Inventor： Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

IPC: G01N21/00

CPC classification number: G01N21/6458 ,  G01N21/6456

Abstract: Noise in a fluorescence image acquired during fluoroscopy is eliminated to present a clear fluorescence image, and the relative positional relationship between the fluoroscopy unit and the specimen can be recognized even while fluoroscopy is in progress. A dark box apparatus for fluoroscopy includes: a dark-box main body enclosing a specimen and a fluoroscopy unit for illuminating the specimen with excitation light with a first spectral band and for detecting fluorescence with a second spectral band generated by the specimen; an illumination light source disposed in the dark-box main body to emit light with a third spectral band different from the first spectral band and the second spectral band; and an observation window disposed in the dark-box main body, the observation window being capable of transmitting light with a fourth spectral band which includes at least part of the third spectral band and does not include the first spectral band and the second spectral band.

Abstract translation: 在荧光透视期间获取的荧光图像中的噪声被消除以呈现清晰的荧光图像，并且即使在进行荧光透视时也能够识别透视单元和样本之间的相对位置关系。 用于荧光透视的暗盒装置包括：包围样本的暗盒主体和用于用具有第一光谱带的激发光照射样本并用于用由样本产生的第二光谱带检测荧光的荧光透视单元; 照明光源，其设置在所述暗盒主体内，以与所述第一光谱带和所述第二光谱带不同的第三光谱带发光; 以及设置在暗箱主体中的观察窗，所述观察窗能够透射具有包括所述第三光谱带的至少一部分的第四光谱带的光，并且不包括所述第一光谱带和所述第二光谱带。

公开(公告)号：US07339667B2

公开(公告)日：2008-03-04

申请号：US11726885

申请日：2007-03-23

Applicant: Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

Inventor： Kei Tsuyuki ,  Kazuhiko Osa ,  Nobuyuki Nagasawa

IPC: G01N21/64

CPC classification number: G01N21/6458 ,  G01N21/6456

Abstract: Noise in a fluorescence image acquired during fluoroscopy is eliminated to present a clear fluorescence image, and the relative positional relationship between the fluoroscopy unit and the specimen can be recognized even while fluoroscopy is in progress. A dark box apparatus for fluoroscopy includes: a dark-box main body enclosing a specimen and a fluoroscopy unit for illuminating the specimen with excitation light with a first spectral band and for detecting fluorescence with a second spectral band generated by the specimen; an illumination light source disposed in the dark-box main body to emit light with a third spectral band different from the first spectral band and the second spectral band; and an observation window disposed in the dark-box main body, the observation window being capable of transmitting light with a fourth spectral band which includes at least part of the third spectral band and does not include the first spectral band and the second spectral band.

Abstract translation: 在荧光透视期间获取的荧光图像中的噪声被消除以呈现清晰的荧光图像，并且即使在进行荧光透视时也能够识别透视单元和样本之间的相对位置关系。 用于荧光透视的暗盒装置包括：包围样本的暗盒主体和用于用具有第一光谱带的激发光照射样本并用于用由样本产生的第二光谱带检测荧光的荧光透视单元; 照明光源，其设置在所述暗盒主体内，以与所述第一光谱带和所述第二光谱带不同的第三光谱带发光; 以及设置在暗箱主体中的观察窗，所述观察窗能够透射具有包括所述第三光谱带的至少一部分的第四光谱带的光，并且不包括所述第一光谱带和所述第二光谱带。

公开(公告)号：US20060214095A1

公开(公告)日：2006-09-28

申请号：US11375042

申请日：2006-03-15

Applicant: Nobuyuki Nagasawa ,  Yoshihisa Tanikawa ,  Kazuhiko Osa ,  Yoshiharu Saito

Inventor： Nobuyuki Nagasawa ,  Yoshihisa Tanikawa ,  Kazuhiko Osa ,  Yoshiharu Saito

IPC: H01J40/14 ,  H01J3/14 ,  H01J5/16

CPC classification number: G02B21/006 ,  A61B5/0059 ,  A61B5/0068 ,  G02B21/0012 ,  G02B21/0028 ,  G02B26/0816

Abstract: It is possible to easily and accurately confirm the position of an optical axis of an objective lens relative to an examination site on a specimen, and positioning of the objective relative to the specimen can be carried out rapidly in a preparation stage. The invention provides scanning examination apparatus comprising a first light source; a light scanning unit configured to scan light from the first light source on a specimen; an objective lens configured to form an image of the light scanned in the light scanning unit at the specimen; a light-detecting unit configured to detect return light emitted from the specimen; a second light source configured to emit visible light; a deflecting optical element, disposed between the light scanning unit and the objective lens, for making visible light emitted from the second light source enter the objective lens along an optical axis of the objective lens; and a beam-shaping unit configured to form the visible light from the second light source, which is irradiated onto a surface of the specimen via the objective lens using the deflecting optical element, into a pattern that enables the optical axis of the objective lens to be indicated.

Abstract translation: 可以容易且准确地确认物镜的光轴相对于检体的检查部位的位置，能够在准备阶段快速地进行物体相对于检体的定位。 本发明提供一种扫描检查装置，包括：第一光源; 光扫描单元，被配置为在样本上扫描来自第一光源的光; 物镜，被配置为在所述样本处形成在所述光扫描单元中扫描的光的图像; 光检测单元，被配置为检测从所述样本发射的返回光; 配置为发射可见光的第二光源; 设置在光扫描单元和物镜之间的偏转光学元件，用于使从第二光源发出的可见光沿着物镜的光轴进入物镜; 以及光束整形单元，被配置为将来自所述第二光源的可见光通过所述偏转光学元件通过所述物镜照射到所述样本的表面上，使得能够使所述物镜的光轴 被指示。

公开(公告)号：US5235375A

公开(公告)日：1993-08-10

申请号：US973561

申请日：1992-11-09

Applicant: Motokazu Yamana ,  Nobuyuki Nagasawa ,  Yasuteru Takahama

Inventor： Motokazu Yamana ,  Nobuyuki Nagasawa ,  Yasuteru Takahama

IPC: G02B7/36

CPC classification number: G02B7/365

Abstract: A driving section relatively drives an optical system and an object in a direction of an optical axis of the optical system. An information detecting section detects information of the object obtained by the optical system. A focusing position detecting section detects evaluation values representing in-focus states of a plurality of positions in a focusing position and positions near the focusing position, in response to a plurality of focusing position calculation methods different from each other, based on the information of said object respectively. An initializing section initializes an optimal focusing position calculation method, of the plurality of focusing position calculation methods, which is most suitable for a focusing operation of the object.

Abstract translation: 驱动部相对于光学系统的光轴的方向驱动光学系统和物体。 信息检测部分检测由光学系统获得的对象的信息。 聚焦位置检测部分响应于彼此不同的多个聚焦位置计算方法，基于所述聚焦位置检测部分的信息来检测表示在聚焦位置和聚焦位置附近的多个位置的聚焦状态的评价值 对象。 初始化部分初始化最适合于对象的聚焦操作的多个聚焦位置计算方法中的最佳聚焦位置计算方法。

公开(公告)号：US08786720B2

公开(公告)日：2014-07-22

申请号：US12332540

申请日：2008-12-11

Applicant: Masahiro Oba ,  Nobuyuki Nagasawa

Inventor： Masahiro Oba ,  Nobuyuki Nagasawa

IPC: H04N5/235

CPC classification number: G06K9/20 ,  G06T5/50 ,  G06T7/0012 ,  G06T2207/20221 ,  H04N5/23232 ,  H04N5/2352

Abstract: When a biological specimen, such as living cells, biological tissue, or a small animal, is quantitatively observed, the specimen is observed for a long time or over a long period, while maintaining the quantitative properties. Provided is an observation method of observing luminescence or fluorescence emitted from a biological specimen. The observation method includes a referring step of referring to image data previously acquired; an intensity determining step of determining whether the intensity of the image data previously acquired is saturated; an exposure dividing step of dividing the exposure of the image data previously acquired one time into a plurality of exposures when the intensity is determined as being saturated in the intensity determining step; an image acquiring step of acquiring images the same number of times as the number of divided exposures; and an integrating step of integrating the images separately acquired multiple times into a single image.

Abstract translation: 当定量观察诸如活细胞，生物组织或小动物的生物样本时，长时间或长时间观察样本，同时保持定量性质。 提供了观察从生物样本发出的发光或荧光的观察方法。 观察方法包括参照先前获取的图像数据的参考步骤; 强度确定步骤，确定先前获取的图像数据的强度是否饱和; 曝光分割步骤，当在所述强度确定步骤中确定所述强度被确定为饱和时，将先前获取的一次的图像数据的曝光除以多个曝光; 图像获取步骤，获取与分割曝光数相同次数的图像; 以及将多次分开获取的图像集成到单个图像中的整合步骤。

公开(公告)号：US20070115543A1

公开(公告)日：2007-05-24

申请号：US11651680

申请日：2007-01-09

Applicant: Nobuyuki Nagasawa ,  Yasunori Makara ,  Hiroya Fukuyama ,  Yoshihisa Tanikawa

Inventor： Nobuyuki Nagasawa ,  Yasunori Makara ,  Hiroya Fukuyama ,  Yoshihisa Tanikawa

IPC: G02B21/00

CPC classification number: A61B5/0059 ,  A61B5/0064 ,  A61B5/6835 ,  A61B5/6844 ,  A61B5/6886 ,  A61B2503/40 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/16

Abstract: An easily viewable examination image in which blurring occurring in an image is reduced without operating an examination optical system in real time matching the motion of a specimen is obtained. There is provided an examination method comprising, prior to examining an examination site of a specimen, acquiring an image of the specimen surface of an examination region including the examination site, over a predetermined time range; extracting a plurality of feature points by processing the acquired image of the specimen surface; calculating a motion trajectory for each of the extracted feature points over the time range; and disposing an optical axis of an examination optical system at a position where the motion trajectory of a feature point disposed in the examination site is minimized.

Abstract translation: 获得其中在图像中发生模糊的轻松可见的检查图像，而不用实际地检查检体光学系统来匹配检体的运动。 提供了一种检查方法，其包括在检查样本的检查部位之前，在预定时间范围内获取包括检查部位的检查区域的样本表面的图像; 通过处理获得的样本表面的图像来提取多个特征点; 在时间范围内计算每个提取的特征点的运动轨迹; 并且将检查光学系统的光轴布置在设置在检查部位的特征点的运动轨迹最小化的位置。

公开(公告)号：US20060193496A1

公开(公告)日：2006-08-31

申请号：US11334708

申请日：2006-01-17

Applicant: Nobuyuki Nagasawa ,  Yoshihisa Tanikawa

Inventor： Nobuyuki Nagasawa ,  Yoshihisa Tanikawa

IPC: G06K9/00

CPC classification number: G02B21/365 ,  G02B7/08 ,  G02B21/0012 ,  G02B21/244

Abstract: The invention enables clear, low-blur examination images to be acquired without operating an examination optical system in real time to match the motion of a specimen. The invention provides an examination method comprising fixing an indicator member that is visible from outside to a surface of the specimen, which is disposed within an examination region of a microscope; acquiring a plurality of images of the surface of the specimen, including the indicator member, at predetermined time intervals; processing the plurality of acquired images to calculate a motion trajectory of the indicator member; and fixing an optical axis of an examination optical system at a position where the motion trajectory of the indicator portion at the examination site of the specimen is minimized, to carry out examination.

Abstract translation: 本发明能够实现清晰，低模糊的检查图像，而不需要实时检查检查光学系统来匹配样本的运动。 本发明提供了一种检查方法，包括将从外部可见的指示器构件固定到放置在显微镜检查区域内的样本表面; 以预定的时间间隔获取包括指示器构件的样本表面的多个图像; 处理多个获取的图像以计算指示器构件的运动轨迹; 将检查光学系统的光轴固定在试样的检查部位的指示部的运动轨迹最小的位置，进行检查。