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    Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both PCR clamp and sensor probe
    1.
    发明授权
    Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both PCR clamp and sensor probe 有权
    使用肽核酸作为PCR夹和传感器探针检测核苷酸突变的方法和试剂盒

    公开(公告)号：US07803543B2

    公开(公告)日：2010-09-28

    申请号：US11624796

    申请日：2007-01-19

    申请人： Chiuan-Chian Chiou ,  Ji-Dung Luo

    发明人： Chiuan-Chian Chiou ,  Ji-Dung Luo

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6858 ,  C12Q2531/113 ,  C12Q2525/107 ,  C12Q2525/186 ,  C12Q2563/107 ,  C12Q2549/126 ,  C12Q2535/131

    摘要： Disclosed herein is a method for determining whether a target polynucleotide sequence contained in a nucleic acid sample has nucleotide variation(s) in a selected region thereof, the steps of which involve the use of a pair of primers that allows the formation of a PCR product having a sequence covering that of the selected region of the target polynucleotide sequence via a PCR process, and a peptide nucleic acid (PNA) that acts as a PCR clamp as well as a sensor probe. Also disclosed herein is a kit for use in determining the presence of nucleotide variation(s) in the target polynucleotide sequence, which includes the pair of primers and the PNA.

    摘要翻译： 本文公开了一种用于确定核酸样品中包含的靶多核苷酸序列是否在其选定区域具有核苷酸变异的方法，其步骤涉及使用允许形成PCR产物的一对引物 具有通过PCR过程覆盖靶多核苷酸序列的选定区域的序列，以及充当PCR夹的肽核酸（PNA）以及传感器探针。 本文还公开了用于确定靶多核苷酸序列中核苷酸变异存在的试剂盒，其包括一对引物和PNA。

    Fetuin-beads, Manufacturing method thereof and method of concentrating and detecting influenza virus by the same
    2.
    发明申请
    Fetuin-beads, Manufacturing method thereof and method of concentrating and detecting influenza virus by the same 审中-公开
    胎儿珠粒，其制造方法以及通过其分离和检测流感病毒的方法

    公开(公告)号：US20130004939A1

    公开(公告)日：2013-01-03

    申请号：US13135280

    申请日：2011-06-30

    申请人： Chung-Ming Chang ,  Chiuan-Chian Chiou

    发明人： Chung-Ming Chang ,  Chiuan-Chian Chiou

    IPC分类号： C12Q1/70

    CPC分类号： G01N33/54313 ,  G01N33/56983 ,  G01N2333/11

    摘要： Fetuin-beads, a manufacturing method thereof and a method of concentrating and detecting influenza virus by the fetuin-beads are disclosed. The method of concentrating and detecting an influenza virus by the fetuin-beads comprises the steps of preparing fetuin-beads; mixing the fetuin-beads and a solution capable of providing salt ions to obtain a fetuin-bead solution; mixing the fetuin-bead solution and a sample comprising the influenza virus to concentrate the influenza virus onto the fetuin-beads to obtain a plurality of fetuin bead-influenza virus combinations; and collecting the fetuin bead-influenza virus combinations and using a virus testing method to detect the influenza virus on the fetuin bead-influenza virus combinations. Therefore, an easy and fast method to detect the influenza virus with timeliness and high accuracy is provided.

    摘要翻译： 公开了胎儿球珠，其制造方法和胎球蛋白珠集中和检测流感病毒的方法。 通过胎球蛋白珠浓缩和检测流感病毒的方法包括制备胎球蛋白珠的步骤; 混合胎球蛋白珠和能够提供盐离子以获得胎球蛋白珠溶液的溶液; 将胎球蛋白珠溶液和包含流感病毒的样品混合以将流感病毒浓缩到胎球蛋白珠上以获得多种胎球蛋白珠流感病毒组合; 并收集胎球蛋白珠流感病毒组合，并使用病毒检测方法检测胎牛血清流感病毒组合中的流感病毒。 因此，提供了一种以及时和高精度检测流感病毒的简便快捷的方法。