利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

15 条记录 (耗时 0.039 秒)

    Transgenic systems for the manufacture of poly(2-hydroxy-butyrate-co-3-hydroxyhexanoate)
    9.
    发明授权
    Transgenic systems for the manufacture of poly(2-hydroxy-butyrate-co-3-hydroxyhexanoate) 有权
    用于制备聚（2-羟基 - 丁酸酯 - 共-3-羟基己酸酯）的转基因系统

    公开(公告)号：US07504556B2

    公开(公告)日：2009-03-17

    申请号：US10703906

    申请日：2003-11-07

    申请人： Lara Madison ,  Gjalt W. Huisman ,  Oliver P. Peoples

    发明人： Lara Madison ,  Gjalt W. Huisman ,  Oliver P. Peoples

    IPC分类号： C12N15/82 ,  C12N15/87 ,  A01H1/00

    CPC分类号： C12N9/88 ,  C12N9/0008 ,  C12P7/625

    摘要： Methods for engineering transgenic organisms that synthesize polyhydroxyalkanoates (PHAs) containing 3-hydroxyhexanoate as comonomer have been developed. These processes are based on genetically engineered bacteria such as Escherichia coli or in plant crops as production systems which include PHA biosynthetic genes from PHA producers. In a preferred embodiment of the method, additional genes are introduced in wild type or transgenic polyhydroxybutyrate (PHB) producers, thereby creating new strains that synthesize 3HH monomers which are incorporated into PHAs. The 3HH monomer preferably is derived in microbial systems using butanol or butyrate as feedstocks, which are precursors of 3-hydroxyhexanoyl-CoA. Pathways for in vivo production of butyrol-CoA specifically encompassing butyryl-CoA dehydrogenase activity are provided.

    摘要翻译： 已经开发了合成含有3-羟基己酸作为共聚单体的聚羟基链烷酸酯（PHA）的转基因生物的工程方法。 这些方法基于遗传工程细菌如大肠杆菌或植物作物，作为包括PHA生物合成基因的生产系统。 在该方法的优选实施方案中，在野生型或转基因聚羟基丁酸酯（PHB）生产者中引入另外的基因，从而产生合成并入PHA的3HH单体的新菌株。 3HH单体优选在使用丁醇或丁酸盐作为原料的微生物系统中衍生，其是3-羟基己酰基-CoA的前体。 提供了具体包含丁酰辅酶A脱氢酶活性的丁酰-coA的体内生产途径。