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    cDNAs and proteins involved in hypoxia, circadian and orphan signal transduction pathways, and methods of use
    2.
    发明授权
    cDNAs and proteins involved in hypoxia, circadian and orphan signal transduction pathways, and methods of use 失效
    涉及缺氧，昼夜节律和孤儿信号转导途径的cDNA和蛋白质，以及使用方法

    公开(公告)号：US07501489B2

    公开(公告)日：2009-03-10

    申请号：US11288720

    申请日：2005-11-29

    申请人： Christopher A. Bradfield ,  Yi Zhong Gu ,  John B. Hogenesch

    发明人： Christopher A. Bradfield ,  Yi Zhong Gu ,  John B. Hogenesch

    IPC分类号： C07K17/00 ,  C07H21/04

    CPC分类号： C07K14/4702

    摘要： The present invention provides isolated nucleic acids and proteins that are new and distinct members of the bHLH-PAS superfamily of transcription regulators. These “MOPs” (members of PAS) are useful in a variety of research, diagnostic and therapeutic applications. Several of the MOPs of the present invention are α-class hypoxia-inducible factors. Several other of the MOPs of the invention are involved in circadian signal transduction.

    摘要翻译： 本发明提供分离的核酸和蛋白质，其是转录调节物的bHLH-PAS超家族的新的和不同的成员。 这些“MOP”（PAS成员）可用于各种研究，诊断和治疗应用。 本发明的几种MOP是α-类缺氧诱导因子。 本发明的其它几种MOP参与昼夜节律信号转导。

    Nucleic acids encoding murine and human Ah receptors
    4.
    发明授权
    Nucleic acids encoding murine and human Ah receptors 失效
    编码鼠和人Ah受体的核酸

    公开(公告)号：US5378822A

    公开(公告)日：1995-01-03

    申请号：US45806

    申请日：1993-04-08

    申请人： Christopher A. Bradfield ,  Kristin M. Dolwick ,  Alan Poland

    发明人： Christopher A. Bradfield ,  Kristin M. Dolwick ,  Alan Poland

    IPC分类号： C07K14/395 ,  C07K14/47 ,  G01N33/53 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C07K14/395 ,  C07K14/47 ,  G01N33/5308 ,  C07K2319/00

    摘要： Murine and human Ah-receptor cDNAs are provided. These molecules can be used to generate large quantities of Ah-receptor protein for use in competitive binding assays for detecting environmental pollutants. Also, the murine and human cDNAs can be used in the generation of recombinant organisms that can serve as biomonitors for environmental pollutants or in regulating gene expression for receptor agonists. Additionally, the cDNAs can be used to detect human and wildlife populations that have high susceptibility to environmental pollutants and polyacrylic aromatic hydrocarbons.

    摘要翻译： 提供鼠和人Ah受体cDNA。 这些分子可用于产生大量的用于检测环境污染物的竞争性结合测定中的Ah-受体蛋白质。 此外，鼠和人类cDNA可用于产生可用作环境污染物的生物监测器或调节受体激动剂的基因表达的重组生物体。 此外，cDNA可用于检测对环境污染物和聚丙烯酸芳烃具有高度敏感性的人类和野生动植物种群。

    Biological assay for detecting agonists to the Ah receptor
    5.
    发明授权
    Biological assay for detecting agonists to the Ah receptor 失效
    用于检测Ah受体激动剂的生物测定

    公开(公告)号：US5650283A

    公开(公告)日：1997-07-22

    申请号：US366051

    申请日：1994-12-29

    申请人： Christopher A. Bradfield ,  Kristin M. Dolwick ,  Lucy A. Carver

    发明人： Christopher A. Bradfield ,  Kristin M. Dolwick ,  Lucy A. Carver

    IPC分类号： C07K14/395 ,  C07K14/47 ,  C07K14/705 ,  G01N33/53

    CPC分类号： C07K14/395 ,  C07K14/47 ,  C07K14/705 ,  G01N33/5308 ,  C07K2319/00

    摘要： Murine and human Ah receptor cDNAs are provided. These molecules can be used to generate large quantities of Ah-receptor protein. The Ah receptor can be inserted into cell systems such as yeast or mammalian, expressed, and used in assays to detect agonists to the Ah receptor. The Ah receptor inserted into such systems can be either the full Ah receptor, the receptor containing deletions at its amino and carboxyl ends, or a chimeric receptor. The chimeric Ah receptor has its binding and dimerization domains replaced with an analogous region from another binding protein.

    摘要翻译： 提供鼠和人Ah受体cDNA。 这些分子可用于产生大量的Ah受体蛋白质。 可以将Ah受体插入到诸如酵母或哺乳动物的细胞系统中，表达并用于检测Ah受体激动剂的测定中。 插入这种系统的Ah受体可以是完全的Ah受体，在其氨基和羧基末端含有缺失的受体或嵌合受体。 嵌合Ah受体的结合和二聚化结构域被来自另一种结合蛋白的类似区域所取代。

    Assay for dioxins
    6.
    发明授权
    Assay for dioxins 失效
    测定二恶英

    公开(公告)号：US5128244A

    公开(公告)日：1992-07-07

    申请号：US300725

    申请日：1989-01-25

    申请人： Alan P. Poland ,  Christopher A. Bradfield ,  Edward N. Glover ,  Andrew S. Kende ,  Frank H. Ebetino

    发明人： Alan P. Poland ,  Christopher A. Bradfield ,  Edward N. Glover ,  Andrew S. Kende ,  Frank H. Ebetino

    IPC分类号： G01N33/53

    CPC分类号： G01N33/5308 ,  Y10S435/975 ,  Y10S436/804 ,  Y10S436/808 ,  Y10S436/822

    摘要： A screening assay for recognizing the presence of dioxins (and other related toxins) in a sample is disclosed. In one aspect of the invention, Ah receptor from mice and a radioactively labelled halogenated dioxin are used in a competitive binding assay to test for the presence of toxins. The label is .sup.125 I substituted directly on the main dioxin structure. The relative binding of the toxin in the samples (in competition with labelled dioxin) for Ah receptor can be compared against standard curves. A kit is provided for running such an assay and a preferred .sup.125 I ligand is provided.

    摘要翻译： 公开了用于识别样品中二恶英（和其他相关毒素）的存在的筛选测定。 在本发明的一个方面，来自小鼠的Ah受体和放射性标记的卤化二恶英用于竞争性结合测定以测试毒素的存在。 标签125I直接取代主要二恶英结构。 样品中的毒素（与标记的二恶英竞争）相对于Ah受体的相对结合可以与标准曲线进行比较。 提供用于进行这种测定的试剂盒，并提供优选的125I配体。