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公开(公告)号：US08143484B2
公开(公告)日：2012-03-27
申请号：US12357284
申请日：2009-01-21
Applicant: Luke Mehlo , Zuo-Yu Zhao
Inventor： Luke Mehlo , Zuo-Yu Zhao
IPC: C12N15/84
CPC classification number: C12N15/8205
Abstract: A method for regulating expression of a virulence gene of Agrobacterium is described. The method comprises the steps of stimulating cereal cells, such as sorghum, so as to produce an active, typically phenolic, compound and exposing the Agrobacterium to this compound. The compound induces expression of the virulence gene of the Agrobacterium, effecting T-DNA transfer from the Agrobacterium to the cereal cells, which are thereby transformed.
Abstract translation: 描述了调节农杆菌毒力基因表达的方法。 该方法包括刺激谷类细胞如高粱的步骤，以产生活性的，通常为酚的化合物，并将土壤杆菌暴露于该化合物。 该化合物诱导土壤杆菌的毒力基因的表达，从而转化从土壤杆菌到谷物细胞的T-DNA转移。
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公开(公告)号：US20090263902A1
公开(公告)日：2009-10-22
申请号：US12357284
申请日：2009-01-21
Applicant: Luke MEHLO , Zuo-Yu Zhao
Inventor： Luke MEHLO , Zuo-Yu Zhao
CPC classification number: C12N15/8205
Abstract: A method for regulating expression of a virulence gene of Agrobacterium is described. The method comprises the steps of stimulating cereal cells, such as sorghum, so as to produce an active, typically phenolic, compound and exposing the Agrobacterium to this compound. The compound induces expression of the virulence gene of the Agrobacterium, effecting T-DNA transfer from the Agrobacterium to the cereal cells, which are thereby transformed.
Abstract translation: 描述了调节农杆菌毒力基因表达的方法。 该方法包括刺激谷类细胞如高粱的步骤，以产生活性的，通常为酚的化合物，并将土壤杆菌暴露于该化合物。 该化合物诱导土壤杆菌的毒力基因的表达，从而转化从土壤杆菌到谷物细胞的T-DNA转移。
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公开(公告)号：US20090293157A1
公开(公告)日：2009-11-26
申请号：US12358158
申请日：2009-01-22
Applicant: Luke Mehlo , Zuo-Yu Zhao
Inventor： Luke Mehlo , Zuo-Yu Zhao
CPC classification number: C12N15/8205 , A01H4/005 , A01H4/008
Abstract: A process for the production and maintenance of pluripotent and/or totipotent progenitor cereal cells from undifferentiated callus cells is described. Production of the progenitor cells takes place via direct organogenesis on a medium containing at least one auxin and at least one cytokinin. For example, the auxin may be 2,4-dichlorophenoxyacetic acid, indole-3-acetic acid, picloran, naphthelenacetic acid, indole-3-propionic acid, indole-3-butyric acid, phenyl acetic acid, benzofuran-3-acetic acid or phenyl butyric acid, and the cytokinin may be benzyl amino purine, benzyladenine, thidiazuron, zeatin, isopentyladenine, trans-zeatin or dimethylallyladenine. Processes for transformation of the undifferentiated callus cells and/or the progenitor cereal cells are also described. Typical cereal cells are sorghum, maize, wheat, barley, millet, rye, canola, alfalfa, triticale and rice.
Abstract translation: 描述了用于从未分化愈伤组织细胞产生和维持多能和/或全能祖先谷物细胞的方法。 在含有至少一种生长素和至少一种细胞分裂素的培养基上通过直接器官发生进行祖细胞的产生。 例如，生长素可以是2,4-二氯苯氧基乙酸，吲哚-3-乙酸，吡咯兰，萘扁
乙酸，吲哚-3-丙酸，吲哚-3-丁酸，苯乙酸，苯并呋喃-3-乙酸 或苯基丁酸，细胞分裂素可以是苄基氨基嘌呤，苄基腺嘌呤，噻替隆，玉米素，异戊二醛，反式玉米素或二甲基烯丙基。 还描述了用于转化未分化愈伤组织细胞和/或祖细胞谷粒细胞的方法。 典型的谷类细胞是高粱，玉米，小麦，大麦，小米，黑麦，卡诺拉，苜蓿，黑小麦和水稻。-
公开(公告)号：US06720475B1
公开(公告)日：2004-04-13
申请号：US09641111
申请日：2000-08-17
Applicant: Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
Inventor： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
IPC: A01H500
CPC classification number: C12N15/8289 , C12N9/00 , C12N15/8213
Abstract: A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
Abstract translation: 在单子叶植物中，特别是在玉米中有效表达FLP重组酶的核酸序列。 含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。
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公开(公告)号：US08030556B2
公开(公告)日：2011-10-04
申请号：US12174812
申请日：2008-07-17
Applicant: Zuo-Yu Zhao , Ning Wang , Douglas S. Nubel , Shifu Zhen
Inventor： Zuo-Yu Zhao , Ning Wang , Douglas S. Nubel , Shifu Zhen
CPC classification number: A01H5/10
Abstract: A novel double haploid maize line designated PH17AW and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PH17AW with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH17AW through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PH17AW or a trait conversion of PH17AW with another maize line. Inbred maize lines derived from double haploid maize line PH17AW, methods for producing other inbred maize lines derived from double haploid maize line PH17AW and the inbred maize lines and their parts derived by those methods.
Abstract translation: 一种新型双倍体玉米品系，命名为PH17AW，其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍单倍体玉米品系PH17AW。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗入PH17AW的一种或多种性状，以及由此产生的玉米种子，植物和植物部分。 杂交玉米种子，植物或植物部分通过双重单倍体系PH17AW或PH17AW与另一个玉米品系的转化而产生。 衍生自双倍体单倍体玉米品系PH17AW的近交玉米品系，从双倍体单倍体玉米品系PH17AW生产其他近交玉米品系的方法和由这些方法衍生的近交玉米品系及其部分。
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Patent Agency Ranking
公开(公告)号：US07999158B2
公开(公告)日：2011-08-16
申请号：US12534507
申请日：2009-08-03
Applicant: Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
Inventor： Ning Wang , Lynn L. Heetland , William E. Solawetz , Zuo-Yu Zhao
CPC classification number: A01H5/10
Abstract: A novel inbred maize line designated PHWWE and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing inbred maize line PHWWE with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWWE through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the inbred line PHWWE or a trait conversion of PHWWE with another maize line. Inbred maize lines derived from maize line PHWWE, methods for producing other inbred maize lines derived from inbred maize line PHWWE and the inbred maize lines and their parts derived by the use of those methods.
Abstract translation: 一种新型近交玉米品系被指定为PHWWE及其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一玉米植物杂交近交玉米品系PHWWE。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWWE中的一种或多种性状，以及由此生产的玉米种子，植物和植物部分。 杂交玉米种子，植物或植物部分通过交叉自交系PHWWE或PHWWE与另一种玉米品系的转化而产生。 衍生自玉米系PHWWE的近交玉米品系，由近交玉米系PHWWE生产其他近交玉米品系的方法以及通过使用这些方法得到的近交玉米品系及其成分。
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公开(公告)号：US20080299658A1
公开(公告)日：2008-12-04
申请号：US11755083
申请日：2007-05-30
Applicant: Zuo-Yu Zhao , Ning Wang
Inventor： Zuo-Yu Zhao , Ning Wang
CPC classification number: A01H5/10
Abstract: A novel double haploid maize line designated PHWVZ and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing double haploid maize line PHWVZ with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PHWVZ through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the double haploid line PHWVZ or a trait conversion of PHWVZ with another maize line. Inbred maize lines derived from double haploid maize line PHWVZ, methods for producing other inbred maize lines derived from double haploid maize line PHWVZ and the inbred maize lines and their parts derived by the use of those method.
Abstract translation: 一种新型双倍体玉米品系，命名为PHWVZ及其种子，植物和植物部分。 用于生产玉米植物的方法，其包括用另一种玉米植物杂交双倍体单倍体玉米品系PHWVZ。 用于生产含有其遗传物质的玉米植物的方法通过回交转化和/或转化渗透到PHWVZ中的一种或多种性状，以及由此产生的玉米种子，植物和植物部分。 杂交玉米种子，植物或植物部分通过双重单倍体系PHWVZ或PHWVZ与另一个玉米品系的转化而产生。 来自双倍体单倍体玉米品系PHWVZ的近交玉米品系，由双倍体单倍体玉米品系PHWVZ和近交玉米品系及其部分产生的其他近交玉米品系的生产方法。
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8.发明申请Methods of Transforming Plants and Identifying Parental Origin of a Chromosome In Those Plants 审中-公开在这些植物中转化植物和识别染色体的亲本起源的方法公开(公告)号：US20080131896A1
公开(公告)日：2008-06-05
申请号：US11941106
申请日：2007-11-16
Applicant: Jerome P. Ranch , Wallace A. Marsh , Terry EuClaire Meyer , Dwight T. Tomes , Zuo-Yu Zhao , Gan-Yuan Zhong , Jimei Wang , Jeffery A. Mullen , Christopher J. Scelonge , Glenn S. Cole , John F. Soper
Inventor： Jerome P. Ranch , Wallace A. Marsh , Terry EuClaire Meyer , Dwight T. Tomes , Zuo-Yu Zhao , Gan-Yuan Zhong , Jimei Wang , Jeffery A. Mullen , Christopher J. Scelonge , Glenn S. Cole , John F. Soper
IPC: C12Q1/68
CPC classification number: C12N15/8202 , A01H1/04 , A01H5/10 , C12N15/8201 , C12N15/8205 , C12N15/8207 , C12N15/8209 , C12Q1/6855 , C12Q1/6895 , C12Q2600/156
Abstract: Methods for plant transformation, for improving transformation efficiency, and for producing transgenic plants are provided. The methods comprise crossing a recipient plant from a genetic line of a plant species of interest with a donor plant selected from a transformation competent genetic line of the same plant species or of another closely related plant species to obtain a hybrid plant. Tissues obtained from the hybrid plant are transformation competent. These tissues can then be transformed with one or more nucleotide sequences of interest and selected for transgenic events having the nucleotide sequence of interest integrated within a chromosome derived from the recipient plant. Transformed cells can be selected and transgenic hybrid plants regenerated. The nucleotide sequence of interest can be introgressed into the genetic line from which the original recipient parent was derived, or into other genetic lines. Transformed plants and seeds are additionally provided.
Abstract translation: 提供了用于植物转化，提高转化效率和用于产生转基因植物的方法。 所述方法包括将受感染植物从感兴趣的植物物种的遗传系与选自相同植物物种的转基因遗传系或另一密切相关的植物物种的供体植物杂交以获得杂种植物。 从混合植物获得的组织是转化能力。 然后可以用一个或多个感兴趣的核苷酸序列转化这些组织，并选择用于整合在来自受体植物的染色体内的感兴趣的核苷酸序列的转基因事件。 可以选择转化的细胞，并再生转基因杂交植物。 感兴趣的核苷酸序列可以渗入原始受体亲本来源的基因系，或其他遗传系。 另外提供转化的植物和种子。
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公开(公告)号：US06369298B1
公开(公告)日：2002-04-09
申请号：US09056418
申请日：1998-04-07
Applicant: Tishu Cai , Dorothy A. Pierce , Laura A. Tagliani , Zuo-Yu Zhao
Inventor： Tishu Cai , Dorothy A. Pierce , Laura A. Tagliani , Zuo-Yu Zhao
IPC: A01H100
CPC classification number: C12N15/8205
Abstract: Methods and compositions for the efficient transformation of sorghum is provided. The method involves infection with Agrobacterium, particularly those comprising a super-binary vector. In this manner, any gene of interest can be introduced into the sorghum plant. The transformed gene will be flanked by at least one T-DNA border and present in the transformed sorghum in low copy number. Transformed sorghum, cells, tissues, plants, and seed are also provided. The invention encompasses regenerated, fertile sorghum plants, transgenic seeds produced therefrom, T1 and subsequent generations.
Abstract translation: 提供了高效转化高粱的方法和组成。 该方法涉及用农杆菌感染，特别是包含超二元载体的那些。 以这种方式，可以将任何感兴趣的基因引入高粱植物中。 转化的基因将侧接至少一个T-DNA边界并以低拷贝数存在于转化的高粱中。 还提供了转化的高粱，细胞，组织，植物和种子。 本发明包括再生的，可育的高粱植物，由其产生的转基因种子，T1和后代。
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公开(公告)号：US06175058B1
公开(公告)日：2001-01-16
申请号：US09263128
申请日：1999-03-05
Applicant: Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
Inventor： Christopher L. Baszczynski , Benjamin A. Bowen , Bruce J. Drummond , William J. Gordon-Kamm , David J. Peterson , Gary A. Sandahl , Laura A. Tagliani , Zuo-Yu Zhao , Grace marie St. Clair
IPC: A01H100
CPC classification number: C12N9/00 , C12N15/8213 , C12N15/8289
Abstract: A nucleic acid sequence effectively expressing FLP recombinase in monocot plants, particularly in maize. Stable, transformed maize plants harboring a gene encoding FLP or harboring FRT nucleic acid sequences enable efficient site-directed recombination of nucleic acid sequences in a monocot's genome.
Abstract translation: 在单子叶植物中，特别是在玉米中有效表达FLP重组酶的核酸序列。 含有编码FLP或携带FRT核酸序列的基因的稳定的转化的玉米植物能够在单子叶植物的基因组中有效地进行位点定向重组核酸序列。
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