公开(公告)号：US20190241857A1

公开(公告)日：2019-08-08

申请号：US16261249

申请日：2019-01-29

申请人： Chung Yuan Christian University

发明人： Tzong-Rong Ger ,  Tan-Yueh Chen ,  Chao-Ming Su ,  Yin-Chou Huang ,  Fan Dong ,  Yu-Chi Kuo

IPC分类号： C12M1/26 ,  C12M1/12 ,  C12M1/42 ,  C12N5/00 ,  C12M1/34

CPC分类号： C12M33/00 ,  C12M25/14 ,  C12M35/06 ,  C12M41/12 ,  C12N5/0068 ,  C12N2523/00 ,  C12N2527/00 ,  C12N2533/30 ,  C12N2535/10 ,  C12N2537/00

摘要： A device and method for cultivating cells are provided, wherein a cell cultivating layer is formed on a surface of a substrate, and a temperature-responsive layer having a plurality of temperature-responsive polymer with magnetic objects is formed on a surface of the cell cultivating layer. When a controlling characteristic exerted on the temperature-responsive layer is varied, a plurality of cells can be adhered on the cell cultivating layer or detached therefrom. When the device is utilized, a temperature control step is operated to control environmental temperature at a first temperature for inducing temperature-responsive polymers becoming hydrophobic whereby the plurality of cells are adhered on the cell cultivating layer. In addition, the alternating magnetic field is utilized to rise temperature of the temperature-responsive polymers so that the plurality of cells can be kept being adhered on the cell cultivating layer at a second temperature lower than the first temperature.