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6 条记录 (耗时 0.015 秒)

    Method for washing an immunoassay tray and its apparatus
    1.
    发明授权
    Method for washing an immunoassay tray and its apparatus 失效
    洗涤免疫测定托盘及其装置的方法

    公开(公告)号：US4622076A

    公开(公告)日：1986-11-11

    申请号：US762760

    申请日：1985-08-06

    申请人： Chung-Mei Ling

    发明人： Chung-Mei Ling

    IPC分类号： B01L99/00 ,  B08B1/02

    CPC分类号： B01L99/00 ,  B01L2200/0668 ,  B01L3/5085 ,  Y10S436/809

    摘要： A method for washing an assay tray includes the following steps. A solid pad of absorbent material, such as a layer of cotton sandwiched between a cardboard and a nonwoven fabric, is firstly placed over the wells of an assay tray. The assay tray is then tilted or inverted together with the pad to allow the serum to drain off and be absorbed in the pad. The assay tray is then inserted in a washing device and submerged in the stationary washing liquid. Subsequently, the washing device is moved relative to the washing liquid to let the washing liquid flow freely in and out of the wells of the assay tray to thoroughly wash the beads and the wells, while the beads will be blocked from falling out of the wells by means of the special design of the washing device. The washing device has a broad opened wall which confines a network of ribs formed by intersections of transverse and longitudinal walls. The intersecting portions substantially superimpose the center of the wells respectively. Each intersecting portion is of a size such that the beads will be blocked from falling out when the assay tray is inserted in the washing device, while the washing liquid flows freely in and out of the wells.

    摘要翻译： 洗涤分析托盘的方法包括以下步骤。 吸收材料的固体垫，例如夹在纸板和非织造织物之间的一层棉花，首先放置在测定盘的孔上。 然后将测定盘与垫一起倾斜或倒置，以使血清排出并吸收在垫中。 然后将测定盘插入洗涤装置中并浸没在固定的洗涤液中。 随后，洗涤装置相对于洗涤液体移动，使得洗涤液体自由地流入和流出测定托盘的孔，以彻底清洗珠子和孔，同时珠粒将被阻止从孔中脱落 通过洗涤装置的特殊设计。 洗涤装置具有宽的敞开的壁，其限制由横向壁和纵向壁的交叉形成的肋网。 相交部分基本上分别叠加孔的中心。 每个相交部分具有这样的尺寸，使得当测定托盘插入洗涤装置中时，珠粒将被阻挡掉，同时洗涤液体自由地流入和流出孔。

    Immunoassay for class specific antibodies
    4.
    发明授权
    Immunoassay for class specific antibodies 失效
    针对类别特异性抗体的免疫测定

    公开(公告)号：US4273756A

    公开(公告)日：1981-06-16

    申请号：US928651

    申请日：1978-07-28

    申请人： Chung-Mei Ling ,  Richard H. Decker ,  Ruben Chairez

    发明人： Chung-Mei Ling ,  Richard H. Decker ,  Ruben Chairez

    IPC分类号： G01N33/53 ,  A61K39/29 ,  A61K39/44 ,  G01N33/543 ,  G01N33/576 ,  G01N33/68 ,  G01N33/16 ,  A61K39/00 ,  A61K43/00

    CPC分类号： G01N33/576 ,  G01N33/54306 ,  G01N33/6854 ,  Y10S436/82

    摘要： The present invention encompasses a method for determining a class specific antibody to an antigen comprising:(a) binding an antibody for a specific immunoglobulin class to a solid support to form a class specific antibody reagent;(b) reacting the class specific antibody reagent with test sample;(c) removing unbound test sample to provide an antigen reagent, and(d) reacting the antigen reagent with a known antigen and measuring the amount of bound antigen thereby determining the amount of class specific antibody to that antigen.The present invention is useful for differential diagnosis, since the relative amount of class specific antibodies varies with the progress of infection.

    摘要翻译： 本发明包括确定针对抗原的特异性抗体的方法，包括：（a）将特异性免疫球蛋白类型的抗体与固体支持物结合以形成分类特异性抗体试剂; （b）使类别特异性抗体试剂与测试样品反应; （c）去除未结合的测试样品以提供抗原试剂，和（d）使抗原试剂与已知抗原反应并测量结合的抗原的量，从而确定该抗原的特异性抗体的量。 本发明可用于鉴别诊断，因为类别特异性抗体的相对量随感染进展而变化。

    Reagents and methods utilizing labeled Fab bound to antigens
    5.
    发明授权
    Reagents and methods utilizing labeled Fab bound to antigens 失效
    利用标记的Fab与抗原结合的试剂和方法

    公开(公告)号：US4298593A

    公开(公告)日：1981-11-03

    申请号：US68772

    申请日：1979-08-21

    申请人： Chung-Mei Ling

    发明人： Chung-Mei Ling

    IPC分类号： G01N33/563 ,  A61K43/00 ,  G01N33/48 ,  G01T1/00

    CPC分类号： G01N33/563 ,  Y10S435/961 ,  Y10S435/964 ,  Y10S436/804 ,  Y10S436/808 ,  Y10S436/82 ,  Y10S530/806 ,  Y10S530/829 ,  Y10S530/863 ,  Y10S530/866

    摘要： This invention involves an improvement in a method for immunochemically assaying a member of an antigen-antibody binding pair in serum or plasma which utilizes labeled antigen having a plurality of antigenic binding sites as the indicating reagent, the improvement comprising employing the antigen immunochemically bound to labeled Fab as the indicating reagent. This invention also encompasses reagents useful in practicing the above method. These methods and reagents are particularly useful in hepatitis detection.

    摘要翻译： 本发明涉及一种免疫化学测定血清或血浆中抗原 - 抗体结合对成员的方法的改进，其利用具有多个抗原结合位点的标记抗原作为指示试剂，其改进包括使用免疫化学结合到标记的抗原 Fab作为指示试剂。 本发明还包括可用于实施上述方法的试剂。 这些方法和试剂在肝炎检测中特别有用。

    Direct radioimmunoassay for antigens and their antibodies
    6.
    发明授权
    Direct radioimmunoassay for antigens and their antibodies 失效
    用于抗原及其抗体的直接放射免疫测定

    公开(公告)号：US4012494A

    公开(公告)日：1977-03-15

    申请号：US480180

    申请日：1974-06-17

    申请人： Chung-Mei Ling

    发明人： Chung-Mei Ling

    IPC分类号： G01N33/543 ,  G01N33/576 ,  G01N33/00 ,  G01N33/16 ,  G21H5/02

    CPC分类号： G01N33/576 ,  G01N33/54306 ,  Y10S436/804 ,  Y10S436/81 ,  Y10S436/82

    摘要： A method for direct radioimmunoassay of antigens or their associated antibodies utilizing a coated test apparatus comprising adding a serum to be tested for an antigen to test apparatus coated with its antibody; incubating the tubes for from 0.5 to 42 hours; aspirating the contents and washing the same with a Tris-HCl and sodium azide mixture; adding purified I.sup.125 labeled antibody into the tube and incubating for from 1 to 24 hours; aspirating and washing the contents; and counting the tube for I.sup.125 radiation. A similar assay for the antibody may be conducted utilizing an antigen coated apparatus and I.sup.125 labeled antigen. The method for coating these apparatus such as tubes comprises adding the antigen or antibody solution in a Tris-HCl, sodium azide solution into a suitable test tube or well, incubating the tubes at room temperature, aspirating and washing the contents and storing at from between 2.degree. and 25.degree. C. until use.

    摘要翻译： 一种用于使用包被的测试装置直接放射免疫测定抗原或其相关抗体的方法，包括将待测试的血清加入到其抗体包被的测试仪器上; 孵育管0.5至42小时; 吸入内容物并用Tris-HCl和叠氮化钠混合物洗涤; 将纯化的I125标记的抗体加入管中并孵育1至24小时; 吸取和洗涤内容物; 并计数I125辐射管。 可以使用抗原包被的设备和I125标记的抗原来进行抗体的类似测定。 用于涂布这些装置如管的方法包括将抗原或抗体溶液加入到Tris-HCl，叠氮化钠溶液中至合适的试管或孔中，在室温下孵育该管，抽吸和洗涤内容物并从其间储存 2℃和25℃直至使用。