公开(公告)号：US20030091976A1

公开(公告)日：2003-05-15

申请号：US10124626

申请日：2002-04-16

Applicant: Ciphergen Biosystems, Inc.

Inventor： Egisto Boschetti ,  Lisa Bradbury ,  Huw Davies ,  Lee O. Lomas ,  Thang T. Pham ,  Vanitha Thulasiraman ,  Tai-Tung Yip

IPC: C12Q001/00 ,  G01N033/53 ,  G01N033/567

CPC classification number: G01N33/6842 ,  C07K1/16 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  G01N33/6803 ,  G01N2030/8411 ,  H01J49/00 ,  H01J49/0418 ,  G01N30/7233

Abstract: The invention provides methods of monitoring the production of a target polypeptide by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken from multiple cell culture batches or of samples taken at different times from a given batch. The invention additionally provides methods for monitoring the purification of a target polypeptide from a mixture by generating surface enhanced laser desorption/ionization mass spectral profiles of samples taken at various times during a purification process. In addition, the invention relates to methods of identifying conditions that can be used in increasing the scale of a given purification process.

Abstract translation: 本发明提供了通过产生从多个细胞培养物批次或从给定批次的不同时间采集的样品获取的样品的表面增强的激光解吸/电离质谱图来监测目标多肽的产生的方法。 本发明另外提供了通过产生在纯化过程中的不同时间采集的样品的表面增强的激光解吸/电离质谱图来监测来自混合物的目标多肽的纯化的方法。 此外，本发明涉及鉴定可用于增加给定纯化方法的规模的条件的方法。

公开(公告)号：US20020060290A1

公开(公告)日：2002-05-23

申请号：US09812307

申请日：2001-03-19

Applicant: Ciphergen Biosystems, Inc.

Inventor： Thang T. Pham

IPC: H01J049/04

CPC classification number: H01J49/0418 ,  G01N33/6851

Abstract: This invention provides a method for analyzing analytes in a sample by mass spectrometry. The method involves pre-fractionating the sample by size exclusion and/or ion exchange chromatography, applying the sample to an adsorbent attached to the surface of a mass spectrometry probe, and allowing both specific and non-specific adsorption of analytes the adsorbent (e.g., by allowing the sample to dry without washing to remove unbound sample). Then an energy absorbing material is added to the dried sample, and the sample is analyzed by laser desorption/ionization mass spectrometry.

Abstract translation: 本发明提供了通过质谱法分析样品中的分析物的方法。 该方法包括通过尺寸排阻和/或离子交换色谱法对样品进行预分馏，将样品应用于附着于质谱探针表面的吸附剂，并允许分析物的特异性和非特异性吸附吸附剂（例如， 通过允许样品干燥而不洗涤以除去未结合的样品）。 然后将能量吸收材料加入到干燥的样品中，并通过激光解吸/电离质谱分析样品。