公开(公告)号：US20040222373A1

公开(公告)日：2004-11-11

申请号：US10836644

申请日：2004-04-29

申请人： SRI International

发明人： Harald Oser ,  Michael J. Coggiola ,  Steven E. Young ,  David R. Crosley

IPC分类号： H01J049/04

CPC分类号： H01J49/162 ,  H01J49/0445

摘要： A method and apparatus are provided for analyzing an analyte at low concentration in a liquid sample by photoionization and mass spectrometry. An inlet system is provided for direct injection of the liquid sample using a capillary tube. The method and apparatus allow for 20 to 2000-fold improvement of the lower detection limit of an analyte in a liquid sample compared to a conventional liquid chromatography/mass spectrometer apparatus.

摘要翻译： 提供了一种用于通过光电离和质谱法分析液体样品中低浓度分析物的方法和装置。 提供入口系统，用于使用毛细管直接注入液体样品。 与常规液相色谱/质谱仪装置相比，该方法和装置允许液体样品中分析物的检测下限提高20至2000倍。

公开(公告)号：US20040217281A1

公开(公告)日：2004-11-04

申请号：US10806829

申请日：2004-03-22

发明人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

IPC分类号： H01J049/04

CPC分类号： H01J49/164

摘要： A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises: The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising: (a) an ionization enclosure including a passageway configured for delivery of ions to the mass analysis device; (b) means to maintain said ionization enclosure at an ambient pressure of greater than 100 mTorr; (c) a holder configured for maintaining a matrix containing said sample in the ionization enclosure at said ambient pressure; (d) a source of laser energy including means associated with the ionization enclosure for directing the laser energy onto said matrix maintained by the holder at the ambient pressure to desorb and ionize at least a portion of the analyte in the sample, and (e) means for directing at least a portion of the at least one ionized analyte into the passageway. The ambient pressure (AP-MALDI) source is compatible with various mass analyzers, particularly with mass spectrometers and solves many problems associated with conventional MALDI sources operating under vacuum. Atmospheric pressure MALDI is described. The analysis of organic molecules or fragments thereof, particularly biomolecules, e.g., biopolymers and organisms, is described.

公开(公告)号：US20040183006A1

公开(公告)日：2004-09-23

申请号：US10603488

申请日：2003-06-25

发明人： James P. Reilly ,  Kirk S. Boraas ,  Noah P. Christian

IPC分类号： H01J049/04

CPC分类号： H01J49/164 ,  H01J49/0004

摘要： A MALDI mass spectrometer directs a laser shot onto a MALDI sample to generate a sample spectrum which is analyzed to determine if the sample spectrum meets a predetermined criteria. If so, subsequent laser shots are directed to predetermined locations on the MALDI sample. In essence, if the analysis of a previous laser shot indicates that a nullsweet spotnull of the MALDI sample has be located, subsequent laser shots may be directed to areas proximate to the previous shot thereby allowing the sweet spot to be thoroughly sampled. A method of operating a MALDI mass spectrometer is also disclosed.

摘要翻译： MALDI质谱仪将激光射击引导到MALDI样品上以产生样品光谱，其被分析以确定样品光谱是否符合预定标准。 如果是这样，随后的激光照射被引导到MALDI样品上的预定位置。 实质上，如果先前的激光镜头的分析表明已经定位了MALDI样品的“最佳点”，则随后的激光照射可以被定向到接近先前镜头的区域，从而允许将甜点彻底地采样。 还公开了一种操作MALDI质谱仪的方法。

公开(公告)号：US20040118222A1

公开(公告)日：2004-06-24

申请号：US10728297

申请日：2003-12-04

发明人： Timothy J. Cornish ,  Frederick P. Gick

IPC分类号： G01N001/24 ,  G01N011/00 ,  H01J049/40 ,  H01J049/04

CPC分类号： G01N1/2252 ,  G01N1/2202 ,  G01N1/24 ,  G01N2001/2223 ,  H01J49/04

摘要： A modular aerosol sample detection system is provided comprising a sample collector couplable to an aerial vehicle and provided with at least one retrievable collection sample plate, which controllably rotates to collect aerosol samples on a multiplicity of collection spots arranged in multiple concentric tracks on the collection disk, and a multi-channel TOF removably couplable to the collection sampler to analyze the collected samples.

摘要翻译： 提供了一种模块化气溶胶样品检测系统，其包括可与飞行器耦合的样品收集器，并且设置有至少一个可收集的收集样品板，其可控地旋转以在集合盘上布置在多个同心轨道中的多个收集点上收集气溶胶样品 ，以及多通道TOF可拆卸地耦合到收集采样器以分析收集的样品。

公开(公告)号：US20040113068A1

公开(公告)日：2004-06-17

申请号：US10649350

申请日：2003-08-26

申请人： Biospect Inc.

发明人： Luc Bousse ,  John T. Stults

IPC分类号： H01J049/04

CPC分类号： H01J49/167 ,  B01L3/0268 ,  B01L3/502715 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/027 ,  B81B1/008 ,  B81B2201/058 ,  G01N27/44717 ,  H01J49/0018

摘要： A microfluidic chip formed with multiple fluid channels terminating at a common electrospray ionization tip for mass spectrometric analysis. The fluid channels may be formed within a substrate plate that are in fluid communication with corresponding reservoirs. The electrospray tip can be formed along a defined portion of the substrate plate, wherein the electrospray tip includes an open-tip region at which the fluid channels converge. A top laminate plate may substantially enclose most portions of the fluid channels formed in the bottom polymer plate except for the open-tip region. Another aspect of the invention provides methods for conducting mass spectrometric analysis of multiple samples flowing through individual fluid channels in a single microfluidic chip that is formed with a convergent electrospray tip. The convergent electrospray tip includes an open or exposed distal pointed tip region.

公开(公告)号：US20030213905A1

公开(公告)日：2003-11-20

申请号：US10146134

申请日：2002-05-16

发明人： John Lennon ,  Taraneh Norouzi

IPC分类号： H01J049/04

CPC分类号： G01N35/1016 ,  B01L3/0262 ,  B01L2400/022 ,  B01L2400/0487 ,  G01N35/1004 ,  G01N2035/102 ,  H01J49/00

摘要： An automated pipetting apparatus and method for forming sample spots on a support include a pipette. A robotic assembly moves a sample container, such as a multiwell microtiter plate, and the support to the pipette for receiving and dispensing liquid samples. The pipette draws a predetermined volume of the liquid sample from the sample container into the axial passage of the pipette and forms a pocket of a gaseous material above and forms a barrier material below the volume of the liquid sample. The liquid sample is drawn into the pipette a distance sufficient to form a space between the liquid sample and the outlet of the pipette and to contain the liquid sample completely within the pipette. The volume of the barrier material, which is typically air or other fluid, separates the liquid sample a suitable distance from the outlet end of the pipette to prevent the liquid sample from flowing outward under gravity and being drawn along the outer surfaces of the pipette by the surface tension of the liquid, thereby preventing or inhibiting evaporation and preventing deposits from forming on the outer surface of the pipette. The pipette dispenses the liquid sample with sufficient force so that substantially the entire volume of the liquid sample is dispensed from the pipette directly onto the support. The support can be, for example, a sample plate of a MALDI mass spectrometer. The pipette deposits the liquid samples to form sample spots on the sample plate for analysis by the mass spectrometer.

摘要翻译： 用于在支撑体上形成样品斑点的自动移液装置和方法包括移液管。 机器人组件将诸如多孔微量滴定板的样品容器移动到移液管上以接收和分配液体样品。 移液管将样品容器中的预定体积的液体样品从吸液管的轴向通道吸入，并在上面形成气态物质的口袋，并在液体样品的体积之下形成阻隔材料。 将液体样品吸入移液管中足以在液体样品和移液管出口之间形成空间并将液体样品完全包含在移液管内的距离。 通常为空气或其他流体的阻隔材料的体积将液体样品与吸液管的出口端隔开合适的距离，以防止液体样品在重力作用下向外流动并沿着吸液管的外表面被拉伸 液体的表面张力，从而防止或抑制蒸发并防止沉积物在移液管的外表面上形成。 移液管以足够的力分配液体样品，使得液体样品的基本上整个体积从移液管直接分配到载体上。 载体可以是例如MALDI质谱仪的样品板。 移液管沉积液体样品，在样品板上形成样品点，用于质谱仪分析。

公开(公告)号：US20030155505A1

公开(公告)日：2003-08-21

申请号：US10081780

申请日：2002-02-20

发明人： Charles W. Russ IV ,  Steven M. Fischer

IPC分类号： H01J049/04

CPC分类号： H01J49/0009 ,  H01J49/004

摘要： An apparatus and method for calibrating a mass spectrometer by internally introducing calibration masses at a post-source stage of the mass spectrometer is provided. A source of lock mass ions adjacent the ion optics creates lock mass ions within the ion optics. Lock mass ions mix with the analyte ions in the ion optics prior to mass analysis. The source of lock mass ions may include various means for ionizing lock mass molecules including but not limited to photoionization, field desorption-ionization, electron ionization, and thermal ionization means. An apparatus and method of mass calibrating a tandem mass spectrometer is also provided. The mass calibration apparatus includes a collision cell for fragmenting analyte ions and a source of lock mass ions adjacent said collision cell for creating lock mass ions in the collision cell. A source of lock mass ions may include various means for ionizing lock mass molecules

摘要翻译： 提供了通过在质谱仪的后源阶段内部引入校准质量来校准质谱仪的装置和方法。 与离子光学器件相邻的锁定质量离子源产生离子光学器件内的锁定质子离子。 在质量分析之前，将质子离子与离子光学中的分析物离子混合。 锁定质量离子的来源可以包括用于电离锁定质量分子的各种装置，包括但不限于光电离，场解吸电离，电子离子化和热电离装置。 还提供了质量校准串联质谱仪的装置和方法。 质量校准装置包括用于碎裂分析物离子的碰撞单元和邻近所述碰撞室的锁定质量离子源，用于在碰撞室中产生锁定质量离子。 锁定质量离子的源可以包括用于电离锁定质量分子的各种手段

公开(公告)号：US20030106997A1

公开(公告)日：2003-06-12

申请号：US10302560

申请日：2002-11-21

申请人： Ciphergen Biosystems, Inc.

发明人： Jody Beecher ,  Frank Scheufele ,  Kamen Voivodov ,  Scot Weinberger ,  William Landgraf

IPC分类号： H01J049/04

CPC分类号： H01J49/0418 ,  B01L3/5085 ,  Y10S977/881 ,  Y10S977/891 ,  Y10S977/892 ,  Y10T436/255

摘要： This invention provides a mass spectrometry probe including a substrate having a surface and a film that coats the surface. The film includes openings that define features for the presentation of an analyte. The film also has a lower surface tension that the surface tension of the substrate surface, and has a water contact angle between 120null and 180null.

公开(公告)号：US20030080289A1

公开(公告)日：2003-05-01

申请号：US10003905

申请日：2001-11-01

申请人： Bechtel BWTX Idaho, LLC

发明人： Jill R. Scott ,  Paul L. Tremblay

IPC分类号： H01J049/04

CPC分类号： H01J49/164

摘要： A laser device includes a target position, an optical component separated a distance J from the target position, and a laser energy source separated a distance H from the optical component, distance H being greater than distance J. A laser source manipulation mechanism exhibits a mechanical resolution of positioning the laser source. The mechanical resolution is less than a spatial resolution of laser energy at the target position as directed through the optical component. A vertical and a lateral index that intersect at an origin can be defined for the optical component. The manipulation mechanism can auto align laser aim through the origin during laser source motion. The laser source manipulation mechanism can include a mechanical index. The mechanical index can include a pivot point for laser source lateral motion and a reference point for laser source vertical motion. The target position can be located within an adverse environment including at least one of a high magnetic field, a vacuum system, a high pressure system, and a hazardous zone. The laser source and an electro-mechanical part of the manipulation mechanism can be located outside the adverse environment. The manipulation mechanism can include a Peaucellier linkage.

摘要翻译： 激光装置包括目标位置，从目标位置分离距离J的光学部件和从光学部件分离距离H的激光能量源，距离H大于距离J.激光源操纵机构显示机械 分辨率定位激光源。 机械分辨率小于通过光学部件指向的目标位置处的激光能量的空间分辨率。 可以为光学部件定义与原点相交的垂直和横向折射率。 在激光源运动过程中，操作机构可以自动对齐激光瞄准原点。 激光源操纵机构可以包括机械指数。 机械指标可以包括激光源横向运动的枢轴点和激光源垂直运动的参考点。 目标位置可以位于包括高磁场，真空系统，高压系统和危险区域中的至少一个的不利环境中。 激光源和操作机构的机电部分可以位于不利环境之外。 操纵机构可以包括Peaucellier连接。

公开(公告)号：US20030075680A1

公开(公告)日：2003-04-24

申请号：US10265943

申请日：2002-10-07

发明人： James A. Apffel JR. ,  Mark H. Werlich ,  James L. Bertsch ,  Paul C. Goodley ,  Kent D. Henry

IPC分类号： H01J049/04

CPC分类号： H01J49/145 ,  G01N30/7253 ,  H01J49/0431 ,  H01J49/165 ,  H01J49/168

摘要： A method and apparatus are disclosed wherein a plurality of electric fields and of orthogonal spray configurations of vaporized analyte are so combined as to enhance the efficiency of analyte detection and mass analysis. The invention provides reduced noise and increased signal sensitivity in both API electrospray and APCI operating modes.