公开(公告)号：US09827552B2

公开(公告)日：2017-11-28

申请号：US14333561

申请日：2014-07-17

Applicant: Clemson University

Inventor： R. Kenneth Marcus ,  Kenneth A. Christensen

IPC: B01J20/00 ,  B01J20/281 ,  G01N30/50 ,  G01N33/543 ,  B01J20/28 ,  B01J20/285 ,  B01J20/32 ,  B01D15/20 ,  B01D15/38 ,  C07K1/20 ,  G01N30/88

CPC classification number: B01J20/281 ,  B01D15/206 ,  B01D15/3823 ,  B01J20/28023 ,  B01J20/28052 ,  B01J20/285 ,  B01J20/321 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/56 ,  C07K1/20 ,  G01N30/50 ,  G01N33/54353 ,  G01N33/54393 ,  G01N2030/8813 ,  Y10T436/255

Abstract: A solid phase for use in separation has been modified using an aqueous phase adsorption of a headgroup-modified lipid to generate analyte specific surfaces for use as a stationary phase in separations such as high performance liquid chromatography (HPLC) or solid phase extraction (SPE). The aliphatic moiety of the lipid adsorbs strongly to a hydrophobic solid surface, with the hydrophilic and active headgroups orienting themselves toward the more polar mobile phase, thus allowing for interactions with the desired solutes. The surface modification approach is generally applicable to a diversity of selective immobilization applications such as protein immobilization clinical diagnostics and preparative scale HPLC as demonstrated on capillary-channeled fibers, though the general methodology could be implemented on any hydrophobic solid support material.

公开(公告)号：US20150024511A1

公开(公告)日：2015-01-22

申请号：US14333561

申请日：2014-07-17

Applicant: Clemson University

Inventor： R. Kenneth Marcus ,  Kenneth A. Christensen

IPC: B01J20/281 ,  G01N1/40

CPC classification number: B01J20/281 ,  B01D15/206 ,  B01D15/3823 ,  B01J20/28023 ,  B01J20/28052 ,  B01J20/285 ,  B01J20/321 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/56 ,  C07K1/20 ,  G01N30/50 ,  G01N33/54353 ,  G01N33/54393 ,  G01N2030/8813 ,  Y10T436/255

Abstract: A solid phase for use in separation has been modified using an aqueous phase adsorption of a headgroup-modified lipid to generate analyte specific surfaces for use as a stationary phase in separations such as high performance liquid chromatography (HPLC) or solid phase extraction (SPE). The aliphatic moiety of the lipid adsorbs strongly to a hydrophobic solid surface, with the hydrophilic and active headgroups orienting themselves toward the more polar mobile phase, thus allowing for interactions with the desired solutes. The surface modification approach is generally applicable to a diversity of selective immobilization applications such as protein immobilization clinical diagnostics and preparative scale HPLC as demonstrated on capillary-channeled fibers, though the general methodology could be implemented on any hydrophobic solid support material.

Abstract translation: 已经使用头基修饰的脂质的水相吸附来生成用于分离的固相，以产生用于分离的分析物特异性表面，例如高效液相色谱（HPLC）或固相萃取（SPE） 。 脂类的脂肪族部分强烈地吸附到疏水性固体表面，亲水和活性头基团自身朝着更极性的流动相取向，从而允许与所需溶质的相互作用。 表面改性方法通常适用于选择性固定化应用的多样性，例如在毛细管通道纤维上证明的蛋白质固定化临床诊断和制备型HPLC，尽管通用方法可以在任何疏水性固体支持材料上实施。