公开(公告)号：US09827552B2

公开(公告)日：2017-11-28

申请号：US14333561

申请日：2014-07-17

Applicant: Clemson University

Inventor： R. Kenneth Marcus ,  Kenneth A. Christensen

IPC: B01J20/00 ,  B01J20/281 ,  G01N30/50 ,  G01N33/543 ,  B01J20/28 ,  B01J20/285 ,  B01J20/32 ,  B01D15/20 ,  B01D15/38 ,  C07K1/20 ,  G01N30/88

CPC classification number: B01J20/281 ,  B01D15/206 ,  B01D15/3823 ,  B01J20/28023 ,  B01J20/28052 ,  B01J20/285 ,  B01J20/321 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/56 ,  C07K1/20 ,  G01N30/50 ,  G01N33/54353 ,  G01N33/54393 ,  G01N2030/8813 ,  Y10T436/255

Abstract: A solid phase for use in separation has been modified using an aqueous phase adsorption of a headgroup-modified lipid to generate analyte specific surfaces for use as a stationary phase in separations such as high performance liquid chromatography (HPLC) or solid phase extraction (SPE). The aliphatic moiety of the lipid adsorbs strongly to a hydrophobic solid surface, with the hydrophilic and active headgroups orienting themselves toward the more polar mobile phase, thus allowing for interactions with the desired solutes. The surface modification approach is generally applicable to a diversity of selective immobilization applications such as protein immobilization clinical diagnostics and preparative scale HPLC as demonstrated on capillary-channeled fibers, though the general methodology could be implemented on any hydrophobic solid support material.

公开(公告)号：US09536725B2

公开(公告)日：2017-01-03

申请号：US14171981

申请日：2014-02-04

Applicant: Clemson University ,  Lawrence Berkeley National Laboratory ,  Pacific Northwest National Laboratory

Inventor： R. Kenneth Marcus ,  Charles Derrick Quarles, Jr. ,  Richard E. Russo ,  David W. Koppenaal ,  Charles J. Barinaga ,  Anthony J. Carado

IPC: H01J49/10 ,  G01J3/443 ,  H01J27/16 ,  H01J49/14 ,  H01J49/16

CPC classification number: H01J27/022 ,  G01J3/443 ,  H01J27/16 ,  H01J49/105 ,  H01J49/145 ,  H01J49/165

Abstract: A liquid sampling, atmospheric pressure, glow discharge (LS-APGD) device as well as systems that incorporate the device and methods for using the device and systems are described. The LS-APGD includes a hollow capillary for delivering an electrolyte solution to a glow discharge space. The device also includes a counter electrode in the form of a second hollow capillary that can deliver the analyte into the glow discharge space. A voltage across the electrolyte solution and the counter electrode creates the microplasma within the glow discharge space that interacts with the analyte to move it to a higher energy state (vaporization, excitation, and/or ionization of the analyte).

Abstract translation: 描述了液体采样，大气压，辉光放电（LS-APGD）装置以及结合装置的系统和使用装置和系统的方法。 LS-APGD包括用于将电解质溶液输送到辉光放电空间的中空毛细管。 该装置还包括可以将分析物输送到辉光放电空间中的第二中空毛细管形式的对电极。 电解质溶液和对电极之间的电压在辉光放电空间内产生与分析物相互作用以将其移动到更高能量状态（分析物的蒸发，激发和/或电离）的微质。

公开(公告)号：US20150024511A1

公开(公告)日：2015-01-22

申请号：US14333561

申请日：2014-07-17

Applicant: Clemson University

Inventor： R. Kenneth Marcus ,  Kenneth A. Christensen

IPC: B01J20/281 ,  G01N1/40

CPC classification number: B01J20/281 ,  B01D15/206 ,  B01D15/3823 ,  B01J20/28023 ,  B01J20/28052 ,  B01J20/285 ,  B01J20/321 ,  B01J20/3272 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/56 ,  C07K1/20 ,  G01N30/50 ,  G01N33/54353 ,  G01N33/54393 ,  G01N2030/8813 ,  Y10T436/255

Abstract: A solid phase for use in separation has been modified using an aqueous phase adsorption of a headgroup-modified lipid to generate analyte specific surfaces for use as a stationary phase in separations such as high performance liquid chromatography (HPLC) or solid phase extraction (SPE). The aliphatic moiety of the lipid adsorbs strongly to a hydrophobic solid surface, with the hydrophilic and active headgroups orienting themselves toward the more polar mobile phase, thus allowing for interactions with the desired solutes. The surface modification approach is generally applicable to a diversity of selective immobilization applications such as protein immobilization clinical diagnostics and preparative scale HPLC as demonstrated on capillary-channeled fibers, though the general methodology could be implemented on any hydrophobic solid support material.

Abstract translation: 已经使用头基修饰的脂质的水相吸附来生成用于分离的固相，以产生用于分离的分析物特异性表面，例如高效液相色谱（HPLC）或固相萃取（SPE） 。 脂类的脂肪族部分强烈地吸附到疏水性固体表面，亲水和活性头基团自身朝着更极性的流动相取向，从而允许与所需溶质的相互作用。 表面改性方法通常适用于选择性固定化应用的多样性，例如在毛细管通道纤维上证明的蛋白质固定化临床诊断和制备型HPLC，尽管通用方法可以在任何疏水性固体支持材料上实施。

公开(公告)号：US11366066B2

公开(公告)日：2022-06-21

申请号：US16600363

申请日：2019-10-11

Applicant: Battelle Memorial Institute ,  Clemson University ,  GAA Custom Engineering, LLC

Inventor： David W. Koppenaal ,  Ying Zhu ,  Edward D. Hoegg ,  R. Kenneth Marcus ,  Gordon A. Anderson ,  Chris Anderson ,  Tyler Williams

IPC: G01N21/67 ,  H01J49/10 ,  H01J37/32 ,  G01N27/68 ,  G01N30/72 ,  G01N27/62 ,  H01J47/12 ,  H01J61/54

Abstract: Apparatus include an atmospheric pressure glow discharge (APGD) analyte electrode defining an analyte discharge axis into an APGD volume, and a plurality of APGD counter electrodes having respective electrical discharge ends directed to the APGD volume, wherein the APGD analyte electrode and the APGD counter electrodes are configured to produce an APGD plasma in the APGD volume with a voltage difference between the APGD analyte electrode and one or more of the AGPD counter electrodes. An electrode can be integrated into an ion inlet. Apparatus can be configured to perform auto-ignition and/or provide multi-modal operation through selectively powering electrodes. Electrode holder devices are disclosed. Related methods are disclosed.

公开(公告)号：US10269525B2

公开(公告)日：2019-04-23

申请号：US15359969

申请日：2016-11-23

Applicant: CLEMSON UNIVERSITY ,  Lawrence Berkeley National Laboratory ,  Pacific Northwest National Laboratory

Inventor： R. Kenneth Marcus ,  Charles Derrick Quarles, Jr. ,  Richard E. Russo ,  David W. Koppenaal ,  Charles J. Barinaga ,  Anthony J. Carado

IPC: H01J27/02 ,  H01J49/10 ,  G01J3/443 ,  H01J27/16 ,  H01J49/14 ,  H01J49/16

Abstract: A liquid sampling, atmospheric pressure, glow discharge (LS-APGD) device as well as systems that incorporate the device and methods for using the device and systems are described. The LS-APGD includes a hollow capillary for delivering an electrolyte solution to a glow discharge space. The device also includes a counter electrode in the form of a second hollow capillary that can deliver the analyte into the glow discharge space. A voltage across the electrolyte solution and the counter electrode creates the microplasma within the glow discharge space that interacts with the analyte to move it to a higher energy state (vaporization, excitation, and/or ionization of the analyte).

公开(公告)号：US20160266134A1

公开(公告)日：2016-09-15

申请号：US15067339

申请日：2016-03-11

Applicant: CLEMSON UNIVERSITY

Inventor： R. Kenneth Marcus ,  Liuwei Jiang

IPC: G01N33/68 ,  G01N33/72 ,  C12Q1/34

CPC classification number: B01J20/3282 ,  B01J20/28023 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3272 ,  B01J2220/84 ,  B01J2220/86 ,  G01N2030/524

Abstract: Separation technologies and a support/separation phases for use therein. A surface of a support phase can be modified to include a crosslinked polymer network as stationary phase to perform separation of one or more species from a liquid in highly efficient separations based on chemical interactions, i.e., chromatography. Optionally, the support phase can employ polymer fibers having channels extending axially along their surfaces. The use of the support phase to support the crosslinked stationary phase can be used in one embodiment in the process of performing micro-scale separations.

Abstract translation: 分离技术和用于其中的支持/分离阶段。 支撑相的表面可以被修饰为包括作为固定相的交联聚合物网络，以基于化学相互作用（即色谱）在高效分离中进行一种或多种物质与液体的分离。 任选地，支持相可以使用具有沿其表面轴向延伸的通道的聚合物纤维。 在执行微尺度分离的过程中，在一个实施方案中可以使用支持相来支持交联的固定相。

公开(公告)号：US11596876B2

公开(公告)日：2023-03-07

申请号：US16267579

申请日：2019-02-05

Applicant: CLEMSON UNIVERSITY RESEARCH FOUNDATION

Inventor： R. Kenneth Marcus ,  Terri F. Bruce ,  Lei Wang ,  Sisi Huang ,  Tyler Y. Slonecki ,  Rhonda Reigers Powell

IPC: B01D15/32 ,  G01N30/00 ,  C07K14/47 ,  C12N9/00 ,  C12N15/10 ,  G01N1/40 ,  G01N33/50 ,  A61K35/68 ,  B01J20/26 ,  B01J20/28 ,  B01J20/285 ,  B01J20/286

Abstract: A relatively fast, inexpensive, and non-destructive method for separation and isolation of biologically active nanoparticles is described. Methods include the use of solid phase separation medis such as channeled fibers in a hydrophobic interaction chromatography (HIC) protocol to isolate biologically active nanoparticles from other components of a mixture. Biologically active nanoparticles can include natural nanoparticles (e.g., exosomes, lysosomes, virus particles) as well as synthetic nanoparticles (liposomes, genetically modified virus particles, etc.)

公开(公告)号：US20210109026A1

公开(公告)日：2021-04-15

申请号：US16600363

申请日：2019-10-11

Applicant: Battelle Memorial Institute ,  Clemson University ,  GAA Custom Engineering, LLC

Inventor： David W. Koppenaal ,  Ying Zhu ,  Edward D. Hoegg ,  R. Kenneth Marcus ,  Gordon A. Anderson ,  Chris Anderson ,  Tyler Williams

IPC: G01N21/67 ,  H01J49/10

Abstract: Apparatus include an atmospheric pressure glow discharge (APGD) analyte electrode defining an analyte discharge axis into an APGD volume, and a plurality of APGD counter electrodes having respective electrical discharge ends directed to the APGD volume, wherein the APGD analyte electrode and the APGD counter electrodes are configured to produce an APGD plasma in the APGD volume with a voltage difference between the APGD analyte electrode and one or more of the AGPD counter electrodes. An electrode can be integrated into an ion inlet. Apparatus can be configured to perform auto-ignition and/or provide multi-modal operation through selectively powering electrodes. Electrode holder devices are disclosed. Related methods are disclosed.

公开(公告)号：US20170162358A1

公开(公告)日：2017-06-08

申请号：US15359969

申请日：2016-11-23

Applicant: CLEMSON UNIVERSITY ,  Lawrence Berkeley National Laboratory ,  Pacific Northwest National Laboratory

Inventor： R. Kenneth Marcus ,  Charles Derrick Quarles, JR. ,  Richard E. Russo ,  David W. Koppenaal ,  Charles J. Barinaga ,  Anthony J. Carado

IPC: H01J27/02 ,  H01J49/10 ,  H01J27/16 ,  G01J3/443

CPC classification number: H01J27/022 ,  G01J3/443 ,  H01J27/16 ,  H01J49/105 ,  H01J49/145 ,  H01J49/165

Abstract: A liquid sampling, atmospheric pressure, glow discharge (LS-APGD) device as well as systems that incorporate the device and methods for using the device and systems are described. The LS-APGD includes a hollow capillary for delivering an electrolyte solution to a glow discharge space. The device also includes a counter electrode in the form of a second hollow capillary that can deliver the analyte into the glow discharge space. A voltage across the electrolyte solution and the counter electrode creates the microplasma within the glow discharge space that interacts with the analyte to move it to a higher energy state (vaporization, excitation, and/or ionization of the analyte).

公开(公告)号：US20140218729A1

公开(公告)日：2014-08-07

申请号：US14171981

申请日：2014-02-04

Applicant: Clemson University ,  Pacific Northwest National Laboratory ,  Lawrence Berkeley National Laboratory

Inventor： R. Kenneth Marcus ,  Charles Derrick Quarles, JR. ,  Richard E. Russo ,  David W. Koppenaal ,  Charles J. Barinaga ,  Anthony J. Carado

IPC: H01J49/10 ,  G01J3/443 ,  H01J27/02

CPC classification number: H01J27/022 ,  G01J3/443 ,  H01J27/16 ,  H01J49/105 ,  H01J49/145 ,  H01J49/165

Abstract: A liquid sampling, atmospheric pressure, glow discharge (LS-APGD) device as well as systems that incorporate the device and methods for using the device and systems are described. The LS-APGD includes a hollow capillary for delivering an electrolyte solution to a glow discharge space. The device also includes a counter electrode in the form of a second hollow capillary that can deliver the analyte into the glow discharge space. A voltage across the electrolyte solution and the counter electrode creates the microplasma within the glow discharge space that interacts with the analyte to move it to a higher energy state (vaporization, excitation, and/or ionization of the analyte).

Abstract translation: 描述了液体采样，大气压，辉光放电（LS-APGD）装置以及结合装置的系统和使用装置和系统的方法。 LS-APGD包括用于将电解质溶液输送到辉光放电空间的中空毛细管。 该装置还包括可以将分析物输送到辉光放电空间中的第二中空毛细管形式的对电极。 电解质溶液和对电极之间的电压在辉光放电空间内产生与分析物相互作用以将其移动到更高能量状态（分析物的蒸发，激发和/或电离）的微质。