公开(公告)号：US20230294086A1

公开(公告)日：2023-09-21

申请号：US18016753

申请日：2021-07-29

Applicant: Colorado State University Research Foundation

Inventor： Charles S. Henry ,  Brian J. Geiss ,  David S. Dandy ,  Cody Carrell ,  Jeremy Link ,  Isabelle Samper ,  Ana Sanchez-Cano ,  Ilhoon Jang ,  Zachary Call

IPC: B01L3/00 ,  G01N33/543

CPC classification number: B01L3/5023 ,  G01N33/54388 ,  B01L3/50273 ,  B01L2200/16 ,  B01L2400/0406 ,  B01L2300/069 ,  B01L2300/088

Abstract: An assay device includes a colorimetric testing assembly including a detection area, a fluid inlet, and a microfluidic network including a first path extending to the detection area and a second path extending to the detection area. When a fluid (e.g., a buffer fluid or a combined buffer and sample solution) is provided to the fluid inlet, a first portion of the fluid rehydrates a first dried reagent (e.g., a dried enzyme label) disposed along the first path to produce a first rehydrated reagent and a second portion of the fluid rehydrates a second dried reagent (e.g., a dried substrate) to produce a second rehydrated reagent. The first rehydrated reagent and the second rehydrated reagent are then sequentially delivered to the detection area by capillary-driven flow to perform the assay.

公开(公告)号：US20230311123A1

公开(公告)日：2023-10-05

申请号：US18042113

申请日：2021-08-19

Applicant: Colorado State University Research Foundation

Inventor： Charles S. Henry ,  Ilhoon Jang

IPC: B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/502776 ,  B01L2200/0621 ,  B01L2400/0481

Abstract: A microfluidic device includes a device body defining a microfluidic pathway including a first channel, a second channel downstream of the first channel, and a junction including a transition between the first channel and the second channel. The transition is configured to inhibit fluid entering the transition from the first channel from forming a meniscus across the second channel, thereby inhibiting capillary-driven flow into the second channel. The microfluidic device further includes a valve that, when activated while capillary-driven flow of the fluid is inhibited at the transition, induces capillary-driven flow through the second channel by facilitating formation of the meniscus.