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    Nucleic Acid Molecules Encoding Novel Human Low-Voltage Activated Calcium Channel Proteins, Designed-Alpha 1I-1 and Alpha 1I-2, Encoded Proteins and Methods of Use Thereof
    2.
    发明申请
    Nucleic Acid Molecules Encoding Novel Human Low-Voltage Activated Calcium Channel Proteins, Designed-Alpha 1I-1 and Alpha 1I-2, Encoded Proteins and Methods of Use Thereof 审中-公开
    编码新型人类低电压活化钙通道蛋白的核酸分子，设计型α1I-1和α1I-2，编码蛋白及其使用方法

    公开(公告)号：US20080248040A1

    公开(公告)日：2008-10-09

    申请号：US10573764

    申请日：2004-09-28

    申请人： Menghang Xia ,  Mark E. Williams

    发明人： Menghang Xia ,  Mark E. Williams

    IPC分类号： A61K39/395 ,  C12N15/11 ,  C07K14/00 ,  C12N15/00 ,  A61K31/70 ,  G01N33/569 ,  C12P21/04 ,  A61P43/00 ,  C12Q1/68 ,  C07K16/18 ,  C12Q1/02 ,  C12N5/06 ,  G01N33/00 ,  G01N33/53

    CPC分类号： C07K14/705

    摘要： Disclosed are mammalian nucleic acid sequences encoding α1I subunit isoforms of a voltage-gated calcium channel. Specifically disclosed are novel variants of the α1I subunit designated herein as α1I-1 and α1I-2. In other aspects, the disclosure relates to expression vectors which encode the novel subunits of the invention, as well as cells containing such vectors. Antibodies specific for each of the variant subunits are also provided. The nucleic acid sequences of the invention find application, for example, in screening for compounds which modulate the activity of voltage-gated calcium channels and also in diagnostic methods for diagnosing various T-type channel mediated disorders, e.g., epilepsy, cancer, pain, sleep disorders and the autoimmune disease Lambert-Eaton Syndrome. Diagnosing defects in α1I subunit genes of a patient with a neuronal disease such as epilepsy are also included. An additional application of the nucleic acid sequences of the invention is in therapeutic methods of treatment for α1I subunit mediated disorders.

    摘要翻译： 公开了编码电压门控钙通道的α1I亚基同种型的哺乳动物核酸序列。 具体公开的是本文称为α1I-1和α1I-2的α1I亚基的新变体。 在其他方面，本公开涉及编码本发明的新亚基的表达载体以及含有这些载体的细胞。 还提供了针对每个变体亚单位特异性的抗体。 本发明的核酸序列可用于例如筛选调节电压门控钙通道活性的化合物，以及用于诊断各种T型通道介导的病症的诊断方法，例如癫痫，癌症，疼痛， 睡眠障碍和自身免疫疾病兰伯特 - 伊顿综合征。 还包括诊断患有神经元疾病如癫痫患者的α1I亚基基因缺陷。 本发明的核酸序列的另外应用是治疗α1I亚基介导的病症的治疗方法。

    Assay methods for state-dependent calcium channel agonists/antagonists
    3.
    发明申请
    Assay methods for state-dependent calcium channel agonists/antagonists 审中-公开
    状态依赖性钙通道激动剂/拮抗剂的测定方法

    公开(公告)号：US20060014206A1

    公开(公告)日：2006-01-19

    申请号：US10530830

    申请日：2003-10-09

    申请人： Menghang Xia ,  Thomas Connolly ,  Paul Bennett ,  Charles Cohen

    发明人： Menghang Xia ,  Thomas Connolly ,  Paul Bennett ,  Charles Cohen

    IPC分类号： G01N33/53

    CPC分类号： C07K14/705 ,  G01N33/6872 ,  G01N2500/10

    摘要： Methods of identifying activators and inhibitors of voltage-gated ion channels are provided in which the methods employ cells transfected with a voltage-gated ion channel of interest and a corollary channel to control the membrane potential of the cells by changing extracellular ion concentration. This allows for more convenient, more precise experimental manipulation of these transitions, and, coupled with efficient methods of detecting the result of ion flux through the channels, provides methods that are especially suitable for high throughput screening.

    摘要翻译： 提供了确定电压门控离子通道的激活剂和抑制剂的方法，其中所述方法使用用感兴趣的电压门控离子通道转染的细胞和必需通道，以通过改变细胞外离子浓度来控制细胞的膜电位。 这允许对这些转变的更方便，更精确的实验操纵，以及与检测通过通道的离子通量结果的有效方法相结合，提供了特别适用于高通量筛选的方法。