公开(公告)号：US20220290123A1

公开(公告)日：2022-09-15

申请号：US17668322

申请日：2022-02-09

申请人： CureVac AG

发明人： Andreas FUNKNER ,  Stefanie SEWING ,  Isabel STROBEL ,  Thorsten MUTZKE

IPC分类号： C12N15/10

摘要： The present invention relates to methods for purifying RNA by chromatography under high salt conditions, e.g. by hydrophobic interaction chromatography.

公开(公告)号：US20180298372A1

公开(公告)日：2018-10-18

申请号：US15580092

申请日：2016-05-30

申请人： CureVac AG

发明人： Andreas FUNKNER ,  Stefanie DORNER ,  Stefanie SEWING ,  Johannes KAMM ,  Norbert BROGHAMMER ,  Thomas KETTERER ,  Thorsten MUTZKE

IPC分类号： C12N15/10 ,  B01D61/14 ,  B01D71/12 ,  G01N30/02

摘要： The present invention relates to method for producing and purifying RNA comprising the steps of providing DNA encoding the RNA; transcription of the DNA into RNA; and conditioning and/or purifying of the solution comprising transcribed RNA by one or more steps of tangential flow filtration (TFF).

公开(公告)号：US20220119795A1

公开(公告)日：2022-04-21

申请号：US17567764

申请日：2022-01-03

申请人： CureVac AG

发明人： Benyamin YAZDAN PANAH ,  Aniela WOCHNER ,  Tilmann ROOS ,  Andreas FUNKNER ,  Martin KUNZE

IPC分类号： C12N15/10 ,  C12Q1/6844 ,  C12P19/34

摘要： The present invention relates to a buffer system comprising a dicarboxylic acid or tricarboxylic acid or a salt thereof for synthesizing RNA molecules as well as a method of RNA in vitro transcription using this buffer system. The present invention also provides the use of this buffer system in RNA in vitro transcription and in the reduction or prevention of precipitates during RNA in vitro transcription.

公开(公告)号：US20200318097A1

公开(公告)日：2020-10-08

申请号：US16464152

申请日：2017-11-28

申请人： CureVac AG

发明人： Andreas FUNKNER ,  Stefanie SEWING ,  Isabel STROBEL ,  Thorsten MUTZKE

IPC分类号： C12N15/10

摘要： The present invention relates to methods for purifying RNA by chromatography under high salt conditions, e.g. by hydrophobic interaction chromatography.

公开(公告)号：US20190010485A1

公开(公告)日：2019-01-10

申请号：US16012751

申请日：2016-12-23

申请人： CureVac AG

发明人： Benyamin YAZDAN PANAH ,  Aniela WOCHNER ,  Tilmann ROOS ,  Andreas FUNKNER ,  Martin KUNZE

IPC分类号： C12N15/10 ,  C12P19/34

摘要： The present invention relates to a buffer system comprising a dicarboxylic acid or tricarboxylic acid or a salt thereof for synthesizing RNA molecules as well as a method of RNA in vitro transcription using this buffer system. The present invention also provides the use of this buffer system in RNA in vitro transcription and in the reduction or prevention of precipitates during RNA in vitro transcription.