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公开(公告)号：US20180237817A1
公开(公告)日：2018-08-23
申请号：US15580121
申请日：2016-05-30
申请人： CureVac AG
发明人： Tilmann ROOS , Benyamin YAZDAN PANAH , Markus CONZELMANN , Andreas THESS , Dominik BUOB , Martin KUNZE , Veronika WAGNER
CPC分类号： C12P19/34 , C12N9/1007 , C12N9/1241 , C12N9/16 , C12N11/02 , C12N11/08 , C12Y201/01056 , C12Y201/01057 , C12Y207/0705 , C12Y301/03033
摘要： The present invention relates to an immobilized capping enzyme, preferably an immobilized Vaccinia virus capping enzyme. Furthermore, the present invention relates to an immobilized cap-specific nucleoside 2′-O-methyltransferase, preferably an immobilized Vaccinia virus cap-specific nucleoside 2′-O-methyltransferase. Moreover, the present invention relates to a method for immobilizing said enzymes and to a method of using said enzymes for the addition of a 5′-cap structure to RNAs. Moreover, the present invention relates to an enzyme reactor for performing the capping reaction using said immobilized enzymes and the subsequent separation of the 5′-capped RNA product. In addition, the present invention relates to a kit comprising the capping enzyme and/or the cap-specific nucleoside 2′-O-methyltransferase.
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公开(公告)号：US20210261897A1
公开(公告)日：2021-08-26
申请号：US17254853
申请日：2019-06-28
发明人： Benyamin YAZDAN PANAH , Tilmann ROOS , Martin KUNZE , Felix BERTSCH , Aniela WOCHNER , Michael RAUEN , Philipp HOFFMANN
摘要： The present invention relates to a bioreactor for RNA in vitro transcription, a method for RNA in vitro transcription, a module for transcribing DNA into RNA and an automated apparatus for RNA manufacturing. Further, the use of a bioreactor for RNA in vitro transcription as described herein is part of the present invention. The present invention relates to an RNA in vitro transcription reactor designed to be operable in an automated manner under GMP-compliant conditions. In particular, said RNA in vitro transcription reactor allows repetitive use of DNA template for various RNA in vitro transcription reactions. Further, the invention relates to an apparatus for RNA manufacturing comprising (a) a module for template DNA synthesis, (b) a module for transcribing DNA into RNA comprising said RNA in vitro transcription reactor, and, optionally, (c) a module for RNA formulation.
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公开(公告)号：US20190083602A1
公开(公告)日：2019-03-21
申请号：US16063999
申请日：2016-12-22
申请人： CureVac AG
IPC分类号： A61K39/12 , C12Q1/6844 , C12N15/10 , C07K14/005 , A61P21/00 , A61P31/00 , A61P39/00 , C40B40/08 , C40B40/02
摘要： The invention relates to a method for producing a ribonucleic acid (RNA) molecule composition comprising n different RNA molecule species, the method comprising a step of RNA in vitro transcription of a mixture of m different deoxyribonucleic acid (DNA) molecule species in a single reaction vessel in parallel, i.e. simultaneously, and a step of obtaining the RNA molecule composition. Also provided is the RNA composition provided by the inventive method and a pharmaceutical composition comprising the same as well as a pharmaceutical container. Moreover, the invention provides the RNA composition and the pharmaceutical composition for use as medicament.
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公开(公告)号：US20220389470A1
公开(公告)日：2022-12-08
申请号：US17820559
申请日：2022-08-17
申请人： CureVac AG
摘要： The present invention relates to a method for in vitro transcription of a linear template DNA which is produced using an immobilized restriction endonuclease. The invention also relates to mutated restriction enzymes which are suitable for immobilization and a solid support to which these restriction enzymes are immobilized. Further, the present invention relates to an enzyme reactor containing said immobilized restriction endonuclease which enzyme reactor can be used for preparing linearized template DNA. Finally, the present invention relates to the use of said enzyme reactor for preparing a linear template DNA for in vitro transcription. In addition, the present invention relates to a kit comprising the immobilized restriction endonuclease.
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公开(公告)号：US20220119795A1
公开(公告)日：2022-04-21
申请号：US17567764
申请日：2022-01-03
申请人： CureVac AG
IPC分类号： C12N15/10 , C12Q1/6844 , C12P19/34
摘要： The present invention relates to a buffer system comprising a dicarboxylic acid or tricarboxylic acid or a salt thereof for synthesizing RNA molecules as well as a method of RNA in vitro transcription using this buffer system. The present invention also provides the use of this buffer system in RNA in vitro transcription and in the reduction or prevention of precipitates during RNA in vitro transcription.
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6.发明申请公开(公告)号：US20190010485A1
公开(公告)日：2019-01-10
申请号：US16012751
申请日：2016-12-23
申请人： CureVac AG
摘要： The present invention relates to a buffer system comprising a dicarboxylic acid or tricarboxylic acid or a salt thereof for synthesizing RNA molecules as well as a method of RNA in vitro transcription using this buffer system. The present invention also provides the use of this buffer system in RNA in vitro transcription and in the reduction or prevention of precipitates during RNA in vitro transcription.
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公开(公告)号：US20190177714A1
公开(公告)日：2019-06-13
申请号：US16087297
申请日：2016-03-24
申请人： CureVac AG
摘要： The present invention relates to an inorganic pyrophosphatase (PPase), methods of producing the same and uses thereof. Further disclosed are an enzyme reactor and a kit comprising the PPase.
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公开(公告)号：US20180208957A1
公开(公告)日：2018-07-26
申请号：US15570130
申请日：2016-04-29
申请人： CureVac AG
摘要： The present invention relates to a method for in vitro transcription of a linear template DNA which is produced using an immobilized restriction endonuclease. The invention also relates to mutated restriction enzymes which are suitable for immobilization and a solid support to which these restriction enzymes are immobilized. Further, the present invention relates to an enzyme reactor containing said immobilized restriction endonuclease which enzyme reactor can be used for preparing linearized template DNA. Finally, the present invention relates to the use of said enzyme reactor for preparing a linear template DNA for in vitro transcription. In addition, the present invention relates to a kit comprising the immobilized restriction endonuclease.
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