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公开(公告)号:US20190133950A1
公开(公告)日:2019-05-09
申请号:US15575301
申请日:2016-05-20
Applicant: CureVac AG
Inventor: Fabian Johannes EBER , Benyamin YAZDAN PANAH , Stefanie SEWIG , Thomas KETTERER , Thorsten MUTZKE , Tilmann ROOS , Michael SONNTAG , Michael WIGGENHORN , Katharina KOLLAND
IPC: A61K9/16 , A61K31/7105 , A61K9/19
Abstract: The present invention is directed to a storage-stable formulation of long-chain RNA. In particular, the invention concerns a dry powder composition comprising a long-chain RNA molecule. The present invention is furthermore directed to methods for preparing a dry powder composition comprising a long-chain RNA molecule by spray-freeze drying. The invention further concerns the use of such a dry powder composition comprising a long-chain RNA molecule in the preparation of pharmaceutical compositions and vaccines, to a method of treating or preventing a disorder or a disease, to first and second medical uses of such a dry powder composition comprising a long-chain RNA molecule and to kits, particularly to kits of parts, comprising such a dry powder composition comprising a long-chain RNA molecule.
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公开(公告)号:US20180237817A1
公开(公告)日:2018-08-23
申请号:US15580121
申请日:2016-05-30
Applicant: CureVac AG
Inventor: Tilmann ROOS , Benyamin YAZDAN PANAH , Markus CONZELMANN , Andreas THESS , Dominik BUOB , Martin KUNZE , Veronika WAGNER
CPC classification number: C12P19/34 , C12N9/1007 , C12N9/1241 , C12N9/16 , C12N11/02 , C12N11/08 , C12Y201/01056 , C12Y201/01057 , C12Y207/0705 , C12Y301/03033
Abstract: The present invention relates to an immobilized capping enzyme, preferably an immobilized Vaccinia virus capping enzyme. Furthermore, the present invention relates to an immobilized cap-specific nucleoside 2′-O-methyltransferase, preferably an immobilized Vaccinia virus cap-specific nucleoside 2′-O-methyltransferase. Moreover, the present invention relates to a method for immobilizing said enzymes and to a method of using said enzymes for the addition of a 5′-cap structure to RNAs. Moreover, the present invention relates to an enzyme reactor for performing the capping reaction using said immobilized enzymes and the subsequent separation of the 5′-capped RNA product. In addition, the present invention relates to a kit comprising the capping enzyme and/or the cap-specific nucleoside 2′-O-methyltransferase.
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公开(公告)号:US20210261897A1
公开(公告)日:2021-08-26
申请号:US17254853
申请日:2019-06-28
Applicant: CureVac AG , Tesla Grohmann Automation GmbH
Inventor: Benyamin YAZDAN PANAH , Tilmann ROOS , Martin KUNZE , Felix BERTSCH , Aniela WOCHNER , Michael RAUEN , Philipp HOFFMANN
Abstract: The present invention relates to a bioreactor for RNA in vitro transcription, a method for RNA in vitro transcription, a module for transcribing DNA into RNA and an automated apparatus for RNA manufacturing. Further, the use of a bioreactor for RNA in vitro transcription as described herein is part of the present invention. The present invention relates to an RNA in vitro transcription reactor designed to be operable in an automated manner under GMP-compliant conditions. In particular, said RNA in vitro transcription reactor allows repetitive use of DNA template for various RNA in vitro transcription reactions. Further, the invention relates to an apparatus for RNA manufacturing comprising (a) a module for template DNA synthesis, (b) a module for transcribing DNA into RNA comprising said RNA in vitro transcription reactor, and, optionally, (c) a module for RNA formulation.
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公开(公告)号:US20200060971A1
公开(公告)日:2020-02-27
申请号:US16679536
申请日:2019-11-11
Applicant: CureVac AG
Inventor: Fabian Johannes EBER , Benyamin YAZDAN PANAH , Stefanie SEWING , Thomas KETTERER , Thorsten MUTZKE , Tilmann ROOS , Michael SONNTAG , Michael WIGGENHORN , Katharina KOLLAND
IPC: A61K9/16 , A61P31/00 , A61P31/12 , A61P37/06 , A61P37/08 , A61P31/04 , A61P33/02 , A61P35/00 , A61K39/145 , A61K48/00
Abstract: The present invention is directed to a storage-stable formulation of long-chain RNA. In particular, the invention concerns a dry powder composition comprising a long-chain RNA molecule. The present invention is furthermore directed to methods for preparing a dry powder composition comprising a long-chain RNA molecule by spray-drying. The invention further concerns the use of such a dry powder composition comprising a long-chain RNA molecule in the preparation of pharmaceutical compositions and vaccines, to a method of treating or preventing a disorder or a disease, to first and second medical uses of such a dry powder composition comprising a long-chain RNA molecule and to kits, particularly to kits of parts, comprising such a dry powder composition comprising a long-chain RNA molecule.
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公开(公告)号:US20190083602A1
公开(公告)日:2019-03-21
申请号:US16063999
申请日:2016-12-22
Applicant: CureVac AG
Inventor: Tilmann ROOS , Martin KUNZE , Benyamin YAZDAN PANAH , Salih YILMAZ , Markus CONZELMANN
IPC: A61K39/12 , C12Q1/6844 , C12N15/10 , C07K14/005 , A61P21/00 , A61P31/00 , A61P39/00 , C40B40/08 , C40B40/02
Abstract: The invention relates to a method for producing a ribonucleic acid (RNA) molecule composition comprising n different RNA molecule species, the method comprising a step of RNA in vitro transcription of a mixture of m different deoxyribonucleic acid (DNA) molecule species in a single reaction vessel in parallel, i.e. simultaneously, and a step of obtaining the RNA molecule composition. Also provided is the RNA composition provided by the inventive method and a pharmaceutical composition comprising the same as well as a pharmaceutical container. Moreover, the invention provides the RNA composition and the pharmaceutical composition for use as medicament.
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公开(公告)号:US20210030683A1
公开(公告)日:2021-02-04
申请号:US16904993
申请日:2020-06-18
Applicant: CureVac AG
Inventor: Fabian Johannes EBER , Benyamin YAZDAN PANAH , Stefanie SEWING , Thomas KETTERER , Thorsten MUTZKE , Tilmann ROOS , Michael SONNTAG , Michael WIGGENHORN , Katharina KOLLAND
IPC: A61K9/16 , A61K48/00 , A61K9/19 , A61K31/7105
Abstract: The present invention is directed to a storage-stable formulation of long-chain RNA. In particular, the invention concerns a dry powder composition comprising a long-chain RNA molecule. The present invention is furthermore directed to methods for preparing a dry powder composition comprising a long-chain RNA molecule by spray-freeze drying. The invention further concerns the use of such a dry powder composition comprising a long-chain RNA molecule in the preparation of pharmaceutical compositions and vaccines, to a method of treating or preventing a disorder or a disease, to first and second medical uses of such a dry powder composition comprising a long-chain RNA molecule and to kits, particularly to kits of parts, comprising such a dry powder composition comprising a long-chain RNA molecule.
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公开(公告)号:US20190177714A1
公开(公告)日:2019-06-13
申请号:US16087297
申请日:2016-03-24
Applicant: CureVac AG
Inventor: Martin KUNZE , Felix Niklas HALDER , Benyamin YAZDAN PANAH , Tilmann ROOS
Abstract: The present invention relates to an inorganic pyrophosphatase (PPase), methods of producing the same and uses thereof. Further disclosed are an enzyme reactor and a kit comprising the PPase.
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公开(公告)号:US20180208957A1
公开(公告)日:2018-07-26
申请号:US15570130
申请日:2016-04-29
Applicant: CureVac AG
Inventor: Tilmann ROOS , Benyamin YAZDAN PANAH , Martin KUNZE
Abstract: The present invention relates to a method for in vitro transcription of a linear template DNA which is produced using an immobilized restriction endonuclease. The invention also relates to mutated restriction enzymes which are suitable for immobilization and a solid support to which these restriction enzymes are immobilized. Further, the present invention relates to an enzyme reactor containing said immobilized restriction endonuclease which enzyme reactor can be used for preparing linearized template DNA. Finally, the present invention relates to the use of said enzyme reactor for preparing a linear template DNA for in vitro transcription. In addition, the present invention relates to a kit comprising the immobilized restriction endonuclease.
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公开(公告)号:US20220389470A1
公开(公告)日:2022-12-08
申请号:US17820559
申请日:2022-08-17
Applicant: CureVac AG
Inventor: Tilmann ROOS , Benyamin YAZDAN PANAH , Martin KUNZE
Abstract: The present invention relates to a method for in vitro transcription of a linear template DNA which is produced using an immobilized restriction endonuclease. The invention also relates to mutated restriction enzymes which are suitable for immobilization and a solid support to which these restriction enzymes are immobilized. Further, the present invention relates to an enzyme reactor containing said immobilized restriction endonuclease which enzyme reactor can be used for preparing linearized template DNA. Finally, the present invention relates to the use of said enzyme reactor for preparing a linear template DNA for in vitro transcription. In addition, the present invention relates to a kit comprising the immobilized restriction endonuclease.
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公开(公告)号:US20220168225A1
公开(公告)日:2022-06-02
申请号:US17676586
申请日:2022-02-21
Applicant: CureVac AG
Inventor: Fabian Johannes EBER , Benyamin YAZDAN PANAH , Stefanie SEWING , Thomas KETTERER , Thorsten MUTZKE , Tilmann ROOS , Michael SONNTAG , Michael WIGGENHORN , Katharina KOLLAND
IPC: A61K9/16 , A61K48/00 , A61K9/19 , A61K31/7105
Abstract: The present invention is directed to a storage-stable formulation of long-chain RNA. In particular, the invention concerns a dry powder composition comprising a long-chain RNA molecule. The present invention is furthermore directed to methods for preparing a dry powder composition comprising a long-chain RNA molecule by spray-freeze drying. The invention further concerns the use of such a dry powder composition comprising a long-chain RNA molecule in the preparation of pharmaceutical compositions and vaccines, to a method of treating or preventing a disorder or a disease, to first and second medical uses of such a dry powder composition comprising a long-chain RNA molecule and to kits, particularly to kits of parts, comprising such a dry powder composition comprising a long-chain RNA molecule.
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