公开(公告)号：US20240288407A1

公开(公告)日：2024-08-29

申请号：US18002002

申请日：2020-06-15

申请人： CUREVAC AG

发明人： Alexander SCHWENGER ,  Stefan HEINZ

IPC分类号： G01N30/86 ,  C12Q1/6806 ,  G01N30/02

CPC分类号： G01N30/8631 ,  C12Q1/6806 ,  G01N2030/027

摘要： The present invention is concerned with a method for determining the integrity of a nucleic acid mixture comprising at least two nucleic acids with different sizes and the use of this method in quality control of a nucleic acid mixture.

公开(公告)号：US20220144877A1

公开(公告)日：2022-05-12

申请号：US16762081

申请日：2018-11-08

申请人： CureVac AG

发明人： Stefan HEINZ ,  Tilmann ROOS ,  Dominik VAHRENHORST ,  Markus CONZELMANN

IPC分类号： C07H1/06 ,  A61K31/713

摘要： The present invention is directed to a method for modifying the retention time of RNA on a chromatographic column. The present invention also concerns a method for purifying RNA from a mixture of at least two RNA species. Furthermore, the present invention relates to a method for co-purifying at least two RNA species from a mixture of at least two RNA species. In particular, the present invention provides a method for harmonizing the numbers of A and/or U nucleotides in at least two RNA species. The present invention is also directed to RNA obtainable by said methods, a composition comprising said RNA or a vaccine comprising said RNA and methods for producing such RNA and compositions. Further, the invention concerns a kit, particularly a kit of parts, comprising the RNA, composition or vaccine. The invention is further directed to a method of treating or preventing a disorder or a disease, first and second medical uses of the RNA, composition and vaccine. Moreover, the present invention concerns a method for providing an adapted RNA sequence or an adapted RNA mixture.

公开(公告)号：US20220073962A1

公开(公告)日：2022-03-10

申请号：US17416681

申请日：2019-12-20

申请人： CureVac AG

发明人： Alexander SCHWENGER ,  Stefan HEINZ ,  Tilmann ROOS ,  Benyamin YAZDAN PANAH ,  Veronika WAGNER

IPC分类号： C12Q1/6806 ,  C12Q1/6876 ,  G01N1/38 ,  G01N30/72

摘要： The present invention is concerned with methods for analyzing RNA molecules. The provided methods involve conjugates for RNA cleavage comprising a chemical moiety with RNA cleaving activity and an oligonucleotide. The oligonucleotide is designed based on a target sequence present in an RNA molecule, and the cleavage of the RNA molecule is inter alia carried out at conditions allowing the hybridization of the oligonucleotide to the target 5 sequence. Thereby, the method is easily applicable to RNA molecules of any sequence. The method further involves the analysis of the RNA fragments obtained after cleavage to obtain information on the physical properties of the RNA molecule.