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    Method of directionally orienting plant embryos
    2.
    发明授权
    Method of directionally orienting plant embryos 失效
    方向定向植物胚胎的方法

    公开(公告)号：US5284765A

    公开(公告)日：1994-02-08

    申请号：US865390

    申请日：1992-04-08

    申请人： Curtis A. Bryan ,  William C. Carlson ,  Michael K. McKinnis

    发明人： Curtis A. Bryan ,  William C. Carlson ,  Michael K. McKinnis

    IPC分类号： A01C1/00 ,  A01C1/06 ,  A01H4/00 ,  C12N5/00 ,  C12N5/02

    CPC分类号： A01H4/00

    摘要： The invention is a method of ordering plant embryos so that a specified end of the embryo is always oriented in a predetermined direction. The embryos are preferably first fairly rapidly desiccated to about 15% moisture content. They are then suspended in a benign liquid flotation medium having a density in the range of about 1.059-1.104 g/cm.sup.3. The density must be adjusted empirically so that a predominant number of viable embryos will float and nonviable embryos will sink. In at least the case of conifer somatic embryos, they will float with the end bearing the latent cotyledons upward. After sufficient separation time in the flotation medium the oriented embryos are swept by a flowing liquid stream into a conduit. They enter cotyledon end first and are then carried to a delivery point without losing that orientation. Here they are separated from the transporting medium. The embryos, still positioned cotyledon end first, may then be picked up by robotic or other means for further processing, such as insertion into an artificial seed.

    摘要翻译： 本发明是一种排序植物胚胎的方法，使得胚胎的指定末端总是沿预定方向取向。 胚胎优选首先相当快速地干燥至约15％的水分含量。 然后将它们悬浮在密度在约1.059-1.104g / cm3范围内的良性液体浮选介质中。 密度必须经验性地调整，使得主要数量的活胚可以漂浮，而不可行胚胎将下沉。 至少在针叶树体细胞胚胎的情况下，它们将浮起，其末端将潜在子叶向上。 在浮选介质中足够的分离时间后，定向胚胎被流动的液体流扫入管道中。 它们首先进入子叶，然后携带到一个传送点，而不会失去该方向。 这里它们与运输介质分离。 然后可以通过机器人或其他手段拾取仍然位于子叶的胚胎，用于进一步加工，例如插入人造种子中。

    Respiration-limited manufactured seed
    3.
    发明授权
    Respiration-limited manufactured seed 失效
    呼吸限定人造种子

    公开(公告)号：US5666762A

    公开(公告)日：1997-09-16

    申请号：US487600

    申请日：1995-06-07

    申请人： William C. Carlson ,  Michael K. McKinnis ,  Jeffrey E. Hartle

    发明人： William C. Carlson ,  Michael K. McKinnis ,  Jeffrey E. Hartle

    IPC分类号： A01C1/00 ,  A01C1/06 ,  A01H4/00 ,  A01H1/00 ,  A01H5/00

    CPC分类号： A01H4/006 ,  Y10S47/09 ,  Y10S47/11

    摘要： The present invention provides manufactured seeds comprising totipotent plant tissue and a hydrated gel, preferably surrounded by a protective seed coat. The cotyledons of the germinating totipotent plant tissue can be enclosed in a shoot restraint that is resistant to penetration by the growing cotyledons. The manufactured seeds can be prepared for long-term storage, e.g., by sparging the hydrated gel with a respiration-limiting gas before, during, or after assembly of the manufactured seed. The seed is then preferably stored in an atmosphere containing levels of a respiration-limiting gas sufficient to keep respiration of the totipotent plant tissue at reduced levels.

    摘要翻译： 本发明提供了包含全能植物组织和水合凝胶的制造种子，优选被保护性种皮围绕。 发芽的全能植物组织的子叶可以封闭在能够被生长的子叶渗透的枝条约束条中。 制备的种子可以制备用于长期储存，例如通过在制造的种子组装之前，期间或之后用呼吸限制气体喷射水合凝胶。 然后优选将种子储存在含有足以保持全能植物组织呼吸降低水平的呼吸限制气体含量的气氛中。

    Upstream engaging fluid switch for serial conveying
    5.
    发明授权
    Upstream engaging fluid switch for serial conveying 失效

    公开(公告)号：US06582159B2

    公开(公告)日：2003-06-24

    申请号：US10071666

    申请日：2002-02-08

    申请人： Michael K. McKinnis

    发明人： Michael K. McKinnis

    IPC分类号： B65G5140

    CPC分类号： A01H4/001 ,  B07C5/362 ,  Y10T137/87804

    摘要： The present invention provides apparatus and methods useful, for example, for introducing a desired spacing between or classifying and sorting objects, e.g. plant embryos. Objects carried serially in a fluid stream enter the apparatus via an upstream conduit. A sensor associated with the conduit provides information regarding an object at a particular location in the upstream conduit and produces a signal. A switch coupled to the upstream conduit directs the fluid stream to an appropriate downstream conduit by applying a force to a conduit, e.g., by aligning the upstream conduit with a downstream conduit to create a fluid-tight path. Apparatus according to the present invention are particularly useful for manipulating fragile multicellular biological objects such as plant embryos.

    Fluid switch
    6.
    发明授权
    Fluid switch 失效
    流体开关

    公开(公告)号：US6145247A

    公开(公告)日：2000-11-14

    申请号：US883757

    申请日：1997-06-27

    申请人： Michael K. McKinnis

    发明人： Michael K. McKinnis

    IPC分类号： A01C1/00 ,  A01H4/00 ,  B07C5/36 ,  A01H1/00 ,  B03B5/28 ,  B65G51/08

    CPC分类号： A01H4/001 ,  B07C5/362 ,  Y10T137/87804

    摘要： The present invention provides apparatus and methods useful, for example, for introducing a desired spacing between or classifying and sorting objects, e.g. plant embryos. Objects carried serially in a fluid stream enter the apparatus via an upstream conduit. A sensor associated with the conduit provides information regarding an object at a particular location in the upstream conduit and produces a signal. A switch coupled to the upstream conduit directs the fluid stream to an appropriate downstream conduit by applying a force to a conduit, e.g., by aligning the upstream conduit with a downstream conduit to create a fluid-tight path. Apparatus according to the present invention are particularly useful for manipulating fragile multicellular biological objects such as plant embryos.

    摘要翻译： 本发明提供了可用于例如用于在物体之间引入期望的间隔或分类和分类物体的装置和方法，例如， 植物胚胎。 在流体流中串联运送的物体经由上游导管进入设备。 与管道相关联的传感器提供关于上游管道中特定位置处的物体的信息并产生信号。 耦合到上游管道的开关通过向导管施加力，例如通过将上游导管与下游导管对准以产生流体密封路径来将流体流引导到合适的下游导管。 根据本发明的装置特别可用于操纵脆弱的多细胞生物物质，如植物胚胎。