公开(公告)号：US11634740B2

公开(公告)日：2023-04-25

申请号：US17788620

申请日：2021-04-27

申请人： DAESANG CORPORATION

发明人： Hyung Seop Youn ,  Eun Seok Choi ,  Ji Ha Lee ,  Suok Su Kim ,  Seung Woo Cho ,  Tae Yong Kim ,  Hak Jun Kim

IPC分类号： C12P19/02 ,  C12P19/24 ,  C12N9/90 ,  C12N15/52

摘要： The present invention provides: a novel allulose epimerase variant in which an amino acid residue present at a specific position of an amino acid sequence of a wild-type D-allulose 3-epimerase derived from Flavonifractor plautii is substituted with another amino acid residue; and various uses of the novel allulose epimerase variant. The novel allulose epimerase variant according to the present invention has a higher conversion rate of fructose to allulose compared to that of the wild-type D-allulose 3-epimerase derived from Flavonifractor plautii, and has excellent thermal stability especially under high temperature conditions of 60° C. or higher, and thus can prevent contamination during an industrial-scale enzymatic conversion reaction for the mass production of allulose, shorten production time, and reduce production costs.

公开(公告)号：US12098404B2

公开(公告)日：2024-09-24

申请号：US17295627

申请日：2020-09-25

申请人： DAESANG CORPORATION

发明人： Hyung Seop Youn ,  Eun Seok Choi ,  Ji Ha Lee ,  Suok Su Kim

IPC分类号： C12P19/24 ,  C12N9/90 ,  C12N15/70 ,  C12P19/02

CPC分类号： C12P19/24 ,  C12N9/90 ,  C12N15/70 ,  C12P19/02 ,  C12Y501/03

摘要： The present invention provides a novel allulose epimerase variant and various uses thereof, in which glycine (Gly), an amino acid residue at position 216 of the amino acid sequence of wild-type D-allulose 3-epimerase derived from Flavonifractor plautii, is substituted with serine (Ser). The novel allulose epimerase variant according to the present invention has a higher conversion activity of fructose to allulose compared to the wild-type D-allulose 3-epimerase derived from Flavonifractor plautii, and in particular, it has excellent thermal stability under high temperature conditions of 60° C. or higher, so that the enzyme conversion reaction is performed at the industrial level for mass production of allulose to prevent contamination, to shorten production time, and to reduce production cost.