公开(公告)号：US11634740B2

公开(公告)日：2023-04-25

申请号：US17788620

申请日：2021-04-27

申请人： DAESANG CORPORATION

发明人： Hyung Seop Youn ,  Eun Seok Choi ,  Ji Ha Lee ,  Suok Su Kim ,  Seung Woo Cho ,  Tae Yong Kim ,  Hak Jun Kim

IPC分类号： C12P19/02 ,  C12P19/24 ,  C12N9/90 ,  C12N15/52

摘要： The present invention provides: a novel allulose epimerase variant in which an amino acid residue present at a specific position of an amino acid sequence of a wild-type D-allulose 3-epimerase derived from Flavonifractor plautii is substituted with another amino acid residue; and various uses of the novel allulose epimerase variant. The novel allulose epimerase variant according to the present invention has a higher conversion rate of fructose to allulose compared to that of the wild-type D-allulose 3-epimerase derived from Flavonifractor plautii, and has excellent thermal stability especially under high temperature conditions of 60° C. or higher, and thus can prevent contamination during an industrial-scale enzymatic conversion reaction for the mass production of allulose, shorten production time, and reduce production costs.

公开(公告)号：US09988618B2

公开(公告)日：2018-06-05

申请号：US15128812

申请日：2015-05-22

申请人： DAESANG CORPORATION

发明人： Tae Gyun Kim ,  Min Su Kim ,  Tae Yong Kim ,  Eun Bum Song ,  Deok Kun Oh

IPC分类号： C12P19/24 ,  C12N9/90 ,  C12N11/00 ,  C12P19/02

CPC分类号： C12N9/90 ,  C12P19/02 ,  C12P19/24 ,  C12Y501/03

摘要： The present invention provides a novel psicose epimerase derived from Flavonifractor plautii and capable of converting fructose to psicose. The novel psicose epimerase according to the present invention possesses an activity producing psicose by epimerizing the carbon-3 position of fructose, and has maximal activity for the conversion of fructose to psicose at a relatively high temperature and a pH less than or equal to neutral, has excellent thermal stability, and can mass-produce psicose from fructose in a high yield for a short amount of time. Therefore, the psicose epimerase according to the present invention is advantageous in the industrial production of psicose, and it is expected that the psicose produced thereby can be usefully utilized in the functional sugar industry and also as materials for health food, medicine, cosmetics, and the like using the psicose.

公开(公告)号：US11447766B2

公开(公告)日：2022-09-20

申请号：US16064644

申请日：2016-12-21

申请人： Daesang Corporation

发明人： Baek Joong Kim ,  Tae Gun Kim ,  Min Su Kim ,  Tae Yong Kim ,  Eun Bum Song

IPC分类号： C12M1/00 ,  C12N11/00 ,  C12N11/10 ,  C12N9/90 ,  C12M1/12 ,  C12M1/26 ,  C12M1/02 ,  C12M1/04 ,  C12N15/77 ,  C12M1/40

摘要： An embodiment of the present disclosure provides an apparatus for immobilizing a microbial cell, the apparatus including: a mixing tank in which a cell-carrier-containing mixed solution is accommodated; a nozzle part through which the cell-carrier-containing mixed solution is injected from the mixing tank and is discharged to the outside; and a reaction tank in which a cell immobilized bead is formed by contact between the cell-carrier-containing mixed solution discharged from the nozzle part and an aqueous curing agent solution. In the apparatus for immobilizing a microbial cell according to the present disclosure, since the cell-carrier-containing mixed solution is injected through an air spraying nozzle, even when an immobilized carrier solution having a high viscosity is used, a microbial cell immobilized bead having a small size and having a spherical shape, or an almost spherical shape may be mass-produced.