公开(公告)号：US20170298400A1

公开(公告)日：2017-10-19

申请号：US15516342

申请日：2015-10-01

申请人： INDUSTRY-ACADEMIC COOPERATION FOUNDATION GYEONGSANG NATIONAL UNIVERSITY

发明人： Seon-Won KIM ,  Min-Jin CHOI ,  Seong-Hee JEONG ,  Dae-Yun LEE

IPC分类号： C12P19/02 ,  C12P19/24

CPC分类号： C12P19/02 ,  C12N9/90 ,  C12P19/24 ,  C12Y501/03 ,  Y02P20/582

摘要： A method of preparing D-psicose includes a step of reacting D-fructose as a substrate and an epimerase thereof in microorganisms at a temperature of 40° C. or higher. The method may further includes inducing the microorganisms to have resting cells by culturing the microorganisms in a medium not containing the D-fructose before the reaction. The method of preparing D-psicose may significantly improve the production amount of D-psicose and production rate of D-psicose.

公开(公告)号：US09701953B2

公开(公告)日：2017-07-11

申请号：US15101459

申请日：2014-07-04

申请人： SAMYANG CORPORATION

发明人： Eun Jin Han ,  Hye Jung Kim ,  Sin Hye Ahn ,  Se-Hui Jeon ,  Chong Jin Park ,  Kang Pyo Lee

IPC分类号： C12N9/90 ,  C12P19/02 ,  C12P19/24

CPC分类号： C12P19/02 ,  C12N9/90 ,  C12P19/24 ,  C12Y501/03

摘要： A psicose 3-epimerase, a polynucleotide encoding the enzyme, a recombinant vector carrying the polynucleotide, a recombinant cell harboring the recombinant vector, and use thereof are provided.

公开(公告)号：US20170101637A1

公开(公告)日：2017-04-13

申请号：US15128812

申请日：2015-05-22

申请人： DAESANG CORPORATION

发明人： Tae Gyun KIM ,  Min Su KIM ,  Tae Yong KIM ,  Eun Bum SONG ,  Deok Kun OH

IPC分类号： C12N9/90 ,  C12P19/02 ,  C12P19/24

CPC分类号： C12N9/90 ,  C12P19/02 ,  C12P19/24 ,  C12Y501/03

摘要： The present invention provides a novel psicose epimerase derived from Flavonifractor plautii and capable of converting fructose to psicose. The novel psicose epimerase according to the present invention possesses an activity producing psicose by epimerizing the carbon-3 position of fructose, and has maximal activity for the conversion of fructose to psicose at a relatively high temperature and a pH less than or equal to neutral, has excellent thermal stability, and can mass-produce psicose from fructose in a high yield for a short amount of time. Therefore, the psicose epimerase according to the present invention is advantageous in the industrial production of psicose, and it is expected that the psicose produced thereby can be usefully utilized in the functional sugar industry and also as materials for health food, medicine, cosmetics, and the like using the psicose.

公开(公告)号：US20160304853A1

公开(公告)日：2016-10-20

申请号：US15101459

申请日：2014-07-04

申请人： SAMYANG CORPORATION

发明人： Eun Jin HAN ,  Hye Jung KIM ,  Sin Hye AHN ,  Se-Hui JEON ,  Chong Jin Park ,  Kang Pyo LEE

IPC分类号： C12N9/90 ,  C12P19/02 ,  C12P19/24

CPC分类号： C12P19/02 ,  C12N9/90 ,  C12P19/24 ,  C12Y501/03

摘要： A psicose 3-epimerase, a polynucleotide encoding the enzyme, a recombinant vector carrying the polynucleotide, a recombinant cell harboring the recombinant vector, and use thereof are provided.

摘要翻译： 提供精神3-差向异构酶，编码该酶的多核苷酸，携带多核苷酸的重组载体，携带重组载体的重组细胞及其用途。

公开(公告)号：US20140199732A1

公开(公告)日：2014-07-17

申请号：US14240647

申请日：2012-08-21

申请人： Yang Hee Kim ,  Jin Ha Kim ,  Young Mi Lee ,  Young Ho Hong ,  Min Hae Kim ,  Seong Bo Kim ,  Seung Won Park ,  Seung Hyun Oh ,  Deok Kun Oh ,  Jin Geun Choi

发明人： Yang Hee Kim ,  Jin Ha Kim ,  Young Mi Lee ,  Young Ho Hong ,  Min Hae Kim ,  Seong Bo Kim ,  Seung Won Park ,  Seung Hyun Oh ,  Deok Kun Oh ,  Jin Geun Choi

IPC分类号： C12P19/24 ,  C12P19/02 ,  C12N9/90

CPC分类号： C12P19/24 ,  C12N9/90 ,  C12P19/02 ,  C12Y501/03

摘要： The present invention relates to a D-psicose 3-epimerase variant with improved thermostability by substituting an amino acid at a specific position of an amino acid sequence of a wild type D-psicose 3-epimerase. Further, the present invention provides a recombinant expression vector including a gene of the D-psicose 3-epimerase variant, and a recombinant strain transformed with the recombinant expression vector. Furthermore, the present invention provides an immobilized reactor prepared using the D-psicose 3-epimerase variant or the recombinant strain, and a method of continuously producing D-psicose using the immobilized reactor.

摘要翻译： 本发明涉及通过用野生型D-灵糖3-差向异构酶的氨基酸序列的特定位置的氨基酸取代而具有改进的热稳定性的D-灵敏的3-差向异构酶变体。 此外，本发明提供了包含D-灵长类3-差向异构酶变体的基因和用重组表达载体转化的重组菌株的重组表达载体。 此外，本发明提供了使用D-灵长类3-差向异构酶变体或重组菌株制备的固定化反应器，以及使用固定化反应器连续生产D-灵敏蛋白的方法。

公开(公告)号：US20190218541A1

公开(公告)日：2019-07-18

申请号：US16370163

申请日：2019-03-29

申请人： Tate & Lyle Ingredients Americas LLC

发明人： Ryan David Woodyer ,  Richard W. Armentrout

IPC分类号： C12N9/90 ,  C12P19/24 ,  C12P19/02

CPC分类号： C12N9/90 ,  C12P19/02 ,  C12P19/24 ,  C12Y501/03

摘要： A protein comprising a polypeptide sequence having at least 70% sequence identity to SEQ ID NO: 6, SEQ ID NO: 2 or SEQ ID NO: 4. The protein has ketose 3-epimerase activity.

公开(公告)号：US09994876B2

公开(公告)日：2018-06-12

申请号：US14864493

申请日：2015-09-24

申请人： Massachusetts Institute of Technology

发明人： Gregory Stephanopoulos ,  Brian Pereira ,  Marjan De Mey ,  Deepak Dugar ,  Jose Luis Avalos

IPC分类号： C12P7/24 ,  C12P7/18 ,  C12N1/21 ,  C12N15/52 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/90 ,  C12N9/88 ,  C12N15/70

CPC分类号： C12P7/24 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0022 ,  C12N9/1096 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/70 ,  C12P7/18 ,  C12P2203/00 ,  C12Y101/01071 ,  C12Y102/01021 ,  C12Y102/01052 ,  C12Y207/01016 ,  C12Y207/01017 ,  C12Y207/01047 ,  C12Y401/02017 ,  C12Y501/03

摘要： The invention relates to recombinant cells and their use in the production of ethylene glycol.

公开(公告)号：US09790481B2

公开(公告)日：2017-10-17

申请号：US14914264

申请日：2014-09-02

申请人： ROQUETTE FRERES

发明人： Pierre Lanos ,  Liuming Zhou ,  Min Jia ,  Wenli Zhang ,  Bo Jiang ,  Wanmeng Mu ,  Tao Zhang

IPC分类号： C12N9/90 ,  C12P19/24 ,  C12P19/02 ,  A23L29/30 ,  C12N1/21 ,  C12N15/61 ,  A23L33/13

CPC分类号： C12N9/90 ,  A23L5/00 ,  A23L29/30 ,  A23L33/13 ,  A23V2002/00 ,  C12P19/02 ,  C12P19/24 ,  C12Y501/03

摘要： The present invention relates to an improved variant of a D-psicose 3-epimerase and its uses.

公开(公告)号：US20160152967A1

公开(公告)日：2016-06-02

申请号：US14786510

申请日：2014-04-23

申请人： CJ CHEILJEDANG CORPORATION

发明人： Young Moon CHO ,  Chang Gyeom KIM ,  Baek Seok LEE

IPC分类号： C12N9/90

CPC分类号： C12N9/90 ,  C12N11/00 ,  C12N15/00 ,  C12P19/02 ,  C12P19/24 ,  C12Y501/03

摘要： The present invention relates to: a D-psicose 3-epimerase mutant from Agrobacterium tumefaciens with improved thermal stability; a recombinant vector comprising a gene encoding the mutant; and a microorganism comprising the mutant. In addition, the present invention relates to a method for producing D-psicose by using the epimerase mutant or the microorganism.

摘要翻译： 本发明涉及：具有改善的热稳定性的来自根癌土壤杆菌的D-灵长类3-差向异构酶突变体; 包含编码突变体的基因的重组载体; 和包含该突变体的微生物。 此外，本发明涉及通过使用差向异构酶突变体或微生物来生产D-灵敏蛋白的方法。

公开(公告)号：US20160076061A1

公开(公告)日：2016-03-17

申请号：US14864493

申请日：2015-09-24

申请人： Massachusetts Institute of Technology

发明人： Gregory Stephanopoulos ,  Brian Pereira ,  Marjan De Mey ,  Deepak Dugar ,  Jose Luis Avalos

IPC分类号： C12P7/24 ,  C12N9/02 ,  C12N9/12 ,  C12P7/18 ,  C12N9/90

CPC分类号： C12P7/24 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0022 ,  C12N9/1096 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/16 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/70 ,  C12P7/18 ,  C12P2203/00 ,  C12Y101/01071 ,  C12Y102/01021 ,  C12Y102/01052 ,  C12Y207/01016 ,  C12Y207/01017 ,  C12Y207/01047 ,  C12Y401/02017 ,  C12Y501/03

摘要： The invention relates to recombinant cells and their use in the production of ethylene glycol.