公开(公告)号：US20240060104A1

公开(公告)日：2024-02-22

申请号：US18269694

申请日：2021-04-26

申请人： DAESANG CORPORATION

发明人： Jong Yun HAN ,  Chel Min YANG ,  Yong Soo KIM ,  Young Il JO

IPC分类号： C12P17/10 ,  C12N9/10

CPC分类号： C12P17/10 ,  C12N9/1077 ,  C12R2001/19 ,  C12Y204/02017

摘要： The present invention relates to an E. coli hisG-derived ATP-phosphoribosyltransferase variant having a reduced feedback inhibition by histidine and a strain expressing the same. The variant may maintain its activity even at a high histidine concentration, thus increasing histidine production.

公开(公告)号：US20230220429A1

公开(公告)日：2023-07-13

申请号：US17864715

申请日：2022-07-14

申请人： DAESANG CORPORATION

发明人： Hyun Ho KIM ,  Sun Hee LEE ,  Dong Hyun KIM ,  Hyun Sook KIM ,  Seok Hyun PARK ,  Joon Hyun PARK

IPC分类号： C12P13/14 ,  C12P13/08 ,  C12N1/20 ,  A23L27/24 ,  A23L27/22

CPC分类号： C12P13/14 ,  C12P13/08 ,  C12N1/205 ,  A23L27/24 ,  A23L27/22 ,  C12R2001/15

摘要： The present invention relates to a method of producing a flavor by a co-fermentation process using mixed fermentation of two or more different microorganisms producing different products. The method of producing a flavor may produce a natural flavor capable of improving the taste and aroma of food and the overall sensory properties of food through a fermentation broth containing amino acids, nucleic acids and/or organic acids, which is produced by mixed fermentation of different microorganisms producing different products, that is, different kinds of amino acids, nucleic acids and/or organic acids. This flavor may be used in various food fields.

公开(公告)号：US11634740B2

公开(公告)日：2023-04-25

申请号：US17788620

申请日：2021-04-27

申请人： DAESANG CORPORATION

发明人： Hyung Seop Youn ,  Eun Seok Choi ,  Ji Ha Lee ,  Suok Su Kim ,  Seung Woo Cho ,  Tae Yong Kim ,  Hak Jun Kim

IPC分类号： C12P19/02 ,  C12P19/24 ,  C12N9/90 ,  C12N15/52

摘要： The present invention provides: a novel allulose epimerase variant in which an amino acid residue present at a specific position of an amino acid sequence of a wild-type D-allulose 3-epimerase derived from Flavonifractor plautii is substituted with another amino acid residue; and various uses of the novel allulose epimerase variant. The novel allulose epimerase variant according to the present invention has a higher conversion rate of fructose to allulose compared to that of the wild-type D-allulose 3-epimerase derived from Flavonifractor plautii, and has excellent thermal stability especially under high temperature conditions of 60° C. or higher, and thus can prevent contamination during an industrial-scale enzymatic conversion reaction for the mass production of allulose, shorten production time, and reduce production costs.

公开(公告)号：US20220411834A1

公开(公告)日：2022-12-29

申请号：US17772813

申请日：2020-06-26

申请人： DAESANG CORPORATION

发明人： Won Joo SHIN ,  Young Il JO ,  Sun Hee LEE ,  Hyun Young KIM ,  Yong Soo KIM ,  Cheol Min YANG

IPC分类号： C12P13/22 ,  C07K14/245 ,  C12N15/70

摘要： Disclosed is a mutant strain having improved aromatic amino acid production capability due to inactivation or weakening of activity of an FMN/FAD exporter protein which is expressed by yeeO gene.

公开(公告)号：US20190002866A1

公开(公告)日：2019-01-03

申请号：US16064644

申请日：2016-12-21

申请人： Daesang Corporation

发明人： Baek Joong KIM ,  Tae Gun KIM ,  Min Su KIM ,  Tae Yong KIM ,  Eun Bum SONG

IPC分类号： C12N11/10 ,  C12N9/90 ,  C12N15/77

摘要： An embodiment of the present disclosure provides an apparatus for immobilizing a microbial cell, the apparatus including: a mixing tank in which a cell-carrier-containing mixed solution is accommodated; a nozzle part through which the cell-carrier-containing mixed solution is injected from the mixing tank and is discharged to the outside; and a reaction tank in which a cell immobilized bead is formed by contact between the cell-carrier-containing mixed solution discharged from the nozzle part and an aqueous curing agent solution. In the apparatus for immobilizing a microbial cell according to the present disclosure, since the cell-carrier-containing mixed solution is injected through an air spraying nozzle, even when an immobilized carrier solution having a high viscosity is used, a microbial cell immobilized bead having a small size and having a spherical shape, or an almost spherical shape may be mass-produced.

公开(公告)号：US20240150801A1

公开(公告)日：2024-05-09

申请号：US18280846

申请日：2022-01-19

申请人： DAESANG CORPORATION

发明人： Moon Jeong KIM ,  Dong Seok LEE ,  Sun Jun YOON ,  Seok Hyun PARK ,  Joon Hyun PARK

IPC分类号： C12P13/10 ,  C12N9/10

CPC分类号： C12P13/10 ,  C12N9/1029 ,  C12Y203/01008

摘要： The present invention relates to a Corynebacterium glutamicum mutant strain having increased L-citrulline productivity, and a method of producing L-citrulline using the same. The Corynebacterium glutamicum mutant strain is capable of producing L-citrulline in high yield and high concentration while inhibiting the production of by-products, because the activity of the protein that is expressed by the NCgl2657 gene therein has been weakened or inactivated.

公开(公告)号：US20240043885A1

公开(公告)日：2024-02-08

申请号：US18490032

申请日：2023-10-19

申请人： DAESANG CORPORATION

发明人： Won Joo SHIN ,  Young II Jo ,  Sun Hee Lee ,  Hyun Young Kim ,  Yong Soo Kim ,  Cheol Min Yang

IPC分类号： C12P13/22 ,  C12N1/20 ,  C12N9/82

CPC分类号： C12P13/22 ,  C12N1/205 ,  C12N9/82 ,  C12Y305/01001 ,  C12R2001/19

摘要： Disclosed is a mutant strain having improved aromatic amino acid production capability as a result of the inactivation or weakening of activity of asparaginase which is expressed by ansB gene.

公开(公告)号：US20230136217A1

公开(公告)日：2023-05-04

申请号：US17798792

申请日：2020-08-26

申请人： DAESANG CORPORATION

发明人： Young Ju LEE ,  Bong Ki KIM ,  Min Jin CHOI ,  Seok Hyun PARK ,  Jae Chun HAN

IPC分类号： C12N1/20 ,  C12N15/77

摘要： The present invention relates to a Corynebacterium glutamicum mutant strain having increased L-glutamic acid productivity, a method for constructing the same, and a method of producing L-glutamic acid using the same. The Corynebacterium glutamicum mutant strain is a strain into which a mechanosensitive ion channel gene derived from a Corynebacterium sp. strain has been introduced, and thus it can produce L-glutamic acid in an improved yield due to enhancement of glutamic acid release. Therefore, when the mutant strain is used, it is possible to more effectively produce L-glutamic acid.

公开(公告)号：US20220307062A1

公开(公告)日：2022-09-29

申请号：US17295627

申请日：2020-09-25

申请人： DAESANG CORPORATION

发明人： Hyung Seop YOUN ,  Eun Seok CHOI ,  Ji Ha LEE ,  Suok Su KIM

IPC分类号： C12P19/24 ,  C12N9/90 ,  C12P19/02 ,  C12N15/70

摘要： The present invention provides a novel allulose epimerase variant and various uses thereof, in which glycine (Gly), an amino acid residue at position 216 of the amino acid sequence of wild-type D-allulose 3-epimerase derived from Flavonifractor plautii, is substituted with serine (Ser). The novel allulose epimerase variant according to the present invention has a higher conversion activity of fructose to allulose compared to the wild-type D-allulose 3-epimerase derived from Flavonifractor plautii, and in particular, it has excellent thermal stability under high temperature conditions of 60° C. or higher, so that the enzyme conversion reaction is performed at the industrial level for mass production of allulose to prevent contamination, to shorten production time, and to reduce production cost.

公开(公告)号：US20220064681A1

公开(公告)日：2022-03-03

申请号：US17418072

申请日：2019-12-18

申请人： DAESANG CORPORATION

发明人： Sun Hee LEE ,  Hyun Ho KIM ,  Hyun Young KIM ,  Young II JO ,  Yong Soo KIM ,  Cheol Min YANG ,  Won Joo SHIN

IPC分类号： C12P13/22 ,  C12N1/20 ,  C12P13/04

摘要： The present disclosure relates to an L-amino acid-producing E. coli mutant strain or Corynebacterium glutamicum mutant strain having enhanced L-amino acid productivity, and a method of producing L-amino acid using the same. The L-amino acid-producing E. coli mutant strain and Corynebacterium glutamicum mutant strain according to the present disclosure exhibit an enhanced ability to produce L-amino acid, such as L-tryptophan, L-phenylalanine, L-tyrosine, L-glutamine, L-lysine, L-arginine, L-valine, L-leucine, L-isoleucine, L-threonine, L-histidine, L-serine, or L-citrulline, compared to parent strains thereof, and are capable of producing a high concentration of L-amino acid in high yield.