公开(公告)号：US20240168040A1

公开(公告)日：2024-05-23

申请号：US18283375

申请日：2022-03-21

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Aaron Stella ,  Chang Liu ,  Yuguan Liu ,  Subhasish Purkayastha

IPC: G01N33/94 ,  C12Q1/37 ,  G01N30/02 ,  G01N30/72 ,  G01N33/543

CPC classification number: G01N33/94 ,  C12Q1/37 ,  G01N30/724 ,  G01N33/54326 ,  G01N33/54353 ,  G01N2030/027 ,  G01N2333/96477 ,  G01N2333/976

Abstract: Methods and kits for preparing liquid samples are presently claimed and described. The method may include treating liquid sample with a hydrolysis enzyme, hydrolyzing the liquid sample to prepare a hydrolysate, and purifying the hydrolysate with magnetic based purification. In certain aspects, the hydrolysis enzyme is bound to a magnetic bead or a magnetic particle. Kits for preparing a liquid sample can include a hydrolysis enzyme, magnetic beads or magnetic particles, one or more internal standards, a liquid chromatography column and one or more solvents to be used as mobile phases, one or more calibrant solutions and instructions for use.