公开(公告)号：US20250003929A1

公开(公告)日：2025-01-02

申请号：US18704236

申请日：2022-10-26

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Aaron Stella ,  Bradley Schneider ,  Subhasish Purkayastha ,  Leigh Bedford

IPC: G01N30/72 ,  G01N27/624 ,  G01N30/02 ,  G01N30/86 ,  G01N30/88 ,  G01N33/94

Abstract: Disclosed are methods for detecting and quantifying target analytes in a sample by mass analysis that include detecting the presence of ions of the target analyte in the sample and quantifying the amount of the target analyte. The methods can include one or more standards (e.g., internal and/or external standards) that may be labelled (e.g., isotopically labelled).

公开(公告)号：US20240420939A1

公开(公告)日：2024-12-19

申请号：US18704242

申请日：2022-10-26

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Aaron Stella ,  Michal Weinstock ,  Yongquan Lai ,  Subhasish Purkayastha ,  Scott Daniels

IPC: H01J49/04 ,  H01J49/00

Abstract: The presently claimed and described technology provides a sample processing system comprising at least one sample introduction device, wherein the at least one sample introduction device is configured to receive a sample; a mass analyzer coupled to the sample introduction device; a control system configured to at least control the at least one sample introduction device and/or the mass analyzer, wherein the mass analyzer is configured to perform a first mass analysis on the sample, wherein the first mass analysis is mass screening for an analyte of interest in the sample, and wherein if the analyte of interest is detected in the sample, the mass analyzer is configured to perform a second mass analysis, wherein the second mass analysis is a quantitative analysis, comprising: ionizing the sample; monitoring, by mass spectrometry, at least one product ion transition for the at least one analyte and at least one isotopic ion transition for the at least one analyte; determining intensity and/or abundance of the at least one product ion transition and/or the at least one isotopic ion transition; and quantifying the at least one analyte present in the sample using the intensity and/or abundance of the at least one product ion transition and/or isotopic ion transition.

公开(公告)号：US20240177984A1

公开(公告)日：2024-05-30

申请号：US18283378

申请日：2022-03-21

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Bradley Schneider ,  Leigh Bedford ,  Aaron Stella ,  Subhasish Purkayastha

IPC: H01J49/04 ,  G01N1/38 ,  G01N27/624 ,  G01N33/487

CPC classification number: H01J49/0454 ,  G01N1/38 ,  G01N27/624 ,  G01N33/487

Abstract: Methods of detecting at least one analyte in at least one liquid sample are described. The method includes the steps of desalting the liquid sample, acoustically ejecting the desalted sample into an open-port interface, diluting the desalted sample, and transferring the diluted sample to an ionization source, ionizing the diluted sample, and selecting ions of interest by ion mobility

公开(公告)号：US20240168040A1

公开(公告)日：2024-05-23

申请号：US18283375

申请日：2022-03-21

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Aaron Stella ,  Chang Liu ,  Yuguan Liu ,  Subhasish Purkayastha

IPC: G01N33/94 ,  C12Q1/37 ,  G01N30/02 ,  G01N30/72 ,  G01N33/543

CPC classification number: G01N33/94 ,  C12Q1/37 ,  G01N30/724 ,  G01N33/54326 ,  G01N33/54353 ,  G01N2030/027 ,  G01N2333/96477 ,  G01N2333/976

Abstract: Methods and kits for preparing liquid samples are presently claimed and described. The method may include treating liquid sample with a hydrolysis enzyme, hydrolyzing the liquid sample to prepare a hydrolysate, and purifying the hydrolysate with magnetic based purification. In certain aspects, the hydrolysis enzyme is bound to a magnetic bead or a magnetic particle. Kits for preparing a liquid sample can include a hydrolysis enzyme, magnetic beads or magnetic particles, one or more internal standards, a liquid chromatography column and one or more solvents to be used as mobile phases, one or more calibrant solutions and instructions for use.