公开(公告)号：US20170044558A1

公开(公告)日：2017-02-16

申请号：US15338888

申请日：2016-10-31

Applicant: DOW AGROSCIENCES LLC

Inventor： W. Michael AINLEY ,  James W. BING ,  David R. CORBIN ,  Steven L. EVANS ,  Joseph F. PETOLINO ,  Lakshmi SASTRY-DENT ,  Steven A. THOMPSON ,  Steven R. WEBB ,  Mary E. WELTER ,  Ning ZHOU

IPC: C12N15/82 ,  C12N9/12

CPC classification number: C12N15/8213 ,  C12N9/1241 ,  C12Y207/07047

Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Abstract translation: 本发明要求通过使用锌指核酸酶将外源DNA稳定整合到玉米基因组中的特定基因座E32的方法。 要求保护通过本发明方法转化的玉米植物和植物部分。 本发明可用于产生所需的性状如除草剂抗性，除草剂耐受性，抗虫性，抗虫性，抗病性，抗病性，胁迫耐受性和玉米中的胁迫耐受性E32基因座代表插入外源基因的优越位点，因为天然农艺 表型不受干扰。