公开(公告)号：US20180230485A1

公开(公告)日：2018-08-16

申请号：US15887715

申请日：2018-02-02

Applicant: Dow AgroSciences LLC

Inventor： Ruihua REN ,  Bruce A. NAGEL ,  Liang YE ,  Yanxin Star GAO ,  Ryan GIBSON ,  Sushmitha PAULRAJ ,  Tyler MANSFIELD ,  Jafar MAMMADOV ,  Siva P. KUMPATLA ,  Steven A. THOMPSON

IPC: C12N15/82 ,  C07K14/415 ,  A01H1/02

CPC classification number: C12N15/8287 ,  A01H1/02 ,  C07K14/415 ,  C12N15/8289

Abstract: A newly identified protein that is encoded by a polynucleotide sequence associated with cytoplasmic male sterility restorer activity (Rf3) is described. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to restore cytoplasmic male sterility in plants. Further applications of the newly identified polynucleotide sequence associated with cytoplasmic male sterility restorer activity include a mutation (rf3) which results in cytoplasmic male sterility. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to result in cytoplasmic male sterility in plants. Methods for detecting the cytoplasmic male sterility restorer (Rf3) and the cytoplasmic male sterility (rf3) gene sequences are further described.

公开(公告)号：US20150307896A1

公开(公告)日：2015-10-29

申请号：US14586135

申请日：2014-12-30

Applicant: Dow AgroSciences LLC

Inventor： Ruihua REN ,  Bruce A. NAGEL ,  Liang YE ,  Yanxin Star GAO ,  Ryan GIBSON ,  Sushmitha PAULRAJ ,  Tyler MANSFIELD ,  Jafar MAMMADOV ,  Siva P. KUMPATLA ,  Steven A. THOMPSON

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8287 ,  A01H1/02 ,  C07K14/415 ,  C12N15/8289

Abstract: A newly identified protein that is encoded by a polynucleotide sequence associated with cytoplasmic male sterility restorer activity (Rf3) is described. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to restore cytoplasmic male sterility in plants. Further applications of the newly identified polynucleotide sequence associated with cytoplasmic male sterility restorer activity include a mutation (rf3) which results in cytoplasmic male sterility. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to result in cytoplasmic male sterility in plants. Methods for detecting the cytoplasmic male sterility restorer (Rf3) and the cytoplasmic male sterility (rf3) gene sequences are further described.

Abstract translation: 描述了由与细胞质雄性不育恢复活性（Rf 3）相关的多核苷酸序列编码的新鉴定的蛋白质。 细胞质雄性不育恢复基因可以通过育种渗入插入植物基因组中，以恢复植物中的细胞质雄性不育。 与细胞质雄性不育恢复活性相关的新鉴定的多核苷酸序列的进一步应用包括导致细胞质雄性不育的突变（rf3）。 细胞质雄性不育恢复基因可以通过育种渗入插入植物基因组中，导致植物中细胞质雄性不育。 进一步描述了检测细胞质雄性不育恢复基因（Rf3）和细胞质雄性不育（rf3）基因序列的方法。

公开(公告)号：US20200255855A1

公开(公告)日：2020-08-13

申请号：US16823972

申请日：2020-03-19

Applicant: Dow AgroSciences LLC

Inventor： Ruihua REN ,  Bruce A. NAGEL ,  Liang YE ,  Yanxin Star GAO ,  Ryan GIBSON ,  Sushmitha PAULRAJ ,  Tyler MANSFIELD ,  Jafar MAMMADOV ,  Siva P. KUMPATLA ,  Steven A. THOMPSON

IPC: C12N15/82 ,  A01H1/02 ,  C07K14/415

Abstract: A newly identified protein that is encoded by a polynucleotide sequence associated with cytoplasmic male sterility restorer activity (Rf3) is described. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to restore cytoplasmic male sterility in plants. Further applications of the newly identified polynucleotide sequence associated with cytoplasmic male sterility restorer activity include a mutation (rf3) which results in cytoplasmic male sterility. The cytoplasmic male sterility restorer gene can be inserted through breeding introgression into plant genomes to result in cytoplasmic male sterility in plants. Methods for detecting the cytoplasmic male sterility restorer (Rf3) and the cytoplasmic male sterility (rf3) gene sequences are further described.

公开(公告)号：US20170044558A1

公开(公告)日：2017-02-16

申请号：US15338888

申请日：2016-10-31

Applicant: DOW AGROSCIENCES LLC

Inventor： W. Michael AINLEY ,  James W. BING ,  David R. CORBIN ,  Steven L. EVANS ,  Joseph F. PETOLINO ,  Lakshmi SASTRY-DENT ,  Steven A. THOMPSON ,  Steven R. WEBB ,  Mary E. WELTER ,  Ning ZHOU

IPC: C12N15/82 ,  C12N9/12

CPC classification number: C12N15/8213 ,  C12N9/1241 ,  C12Y207/07047

Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Abstract translation: 本发明要求通过使用锌指核酸酶将外源DNA稳定整合到玉米基因组中的特定基因座E32的方法。 要求保护通过本发明方法转化的玉米植物和植物部分。 本发明可用于产生所需的性状如除草剂抗性，除草剂耐受性，抗虫性，抗虫性，抗病性，抗病性，胁迫耐受性和玉米中的胁迫耐受性E32基因座代表插入外源基因的优越位点，因为天然农艺 表型不受干扰。

公开(公告)号：US20140173783A1

公开(公告)日：2014-06-19

申请号：US14105563

申请日：2013-12-13

Applicant: Dow AgroSciences LLC

Inventor： W. Michael AINLEY ,  James W. BING ,  David H. CORBIN ,  Steven L. EVANS ,  Joseph F. PETOLINO ,  Lakshmi SASTRY-DENT ,  Steven A. THOMPSON ,  Steven R. WEBB ,  Mary E. WELTER ,  Ning ZHOU

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N9/1241 ,  C12Y207/07047

Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Abstract translation: 本发明要求通过使用锌指核酸酶将外源DNA稳定整合到玉米基因组中的特定基因座E32中的方法。 要求保护通过本发明方法转化的玉米植物和植物部分。 本发明可用于产生所需的性状如除草剂抗性，除草剂耐受性，抗虫性，抗虫性，抗病性，抗病性，胁迫耐受性和玉米中的胁迫耐受性E32基因座代表插入外源基因的优越位点，因为天然农艺 表型不受干扰。