NOVEL MAIZE UBIQUITIN PROMOTERS
    1.
    发明申请

    公开(公告)号:US20180171345A1

    公开(公告)日:2018-06-21

    申请号:US15887723

    申请日:2018-02-02

    CPC classification number: C12N15/8216 C12N15/10

    Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v2.

    METHODS AND COMPOSITIONS FOR RECOMBINATION A GENE-DEFICIENT STRAINS OF AGROBACTERIUM TUMEFACIENS
    2.
    发明申请
    METHODS AND COMPOSITIONS FOR RECOMBINATION A GENE-DEFICIENT STRAINS OF AGROBACTERIUM TUMEFACIENS 有权
    重组农杆菌基因缺陷菌的方法和组合物

    公开(公告)号:US20160068851A1

    公开(公告)日:2016-03-10

    申请号:US14844731

    申请日:2015-09-03

    CPC classification number: C12N15/8205 C12N15/743

    Abstract: The present disclosure provides novel compositions and methods for the production and use of Agrobacterium tumefaciens strains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains of Agrobacterium tumefaciens are also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided.

    Abstract translation: 本公开提供了用于生产和使用相对于亲本菌株缺乏RecA活性的根癌土壤杆菌菌株(例如LBA4404)的新型组合物和方法。 还公开了与根癌土壤杆菌的其他基因缺陷型菌株的组合。 具体地,提供了两个示例性的recA减毒株,UIA777,其中氯霉素抗性基因破坏recA基因和UIA770,其中卡那霉素抗性基因破坏recA基因。

    Novel Maize Ubiquitin Promoters
    3.
    发明申请
    Novel Maize Ubiquitin Promoters 审中-公开
    新型玉米泛素启动子

    公开(公告)号:US20150184180A1

    公开(公告)日:2015-07-02

    申请号:US14587743

    申请日:2014-12-31

    CPC classification number: C12N15/8216

    Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. LLN37.

    Abstract translation: 玉米罐头 B73泛素-1(Z. mays c.v.B73 Ubi-1)启动子驱动植物中高水平的组成型转基因表达。 重复使用相同的Z. mays c.v. 多基因构建体中的B73 Ubi-1启动子也可导致基因沉默,从而使得转基因产品的效力较差。 提供了使用来自不同Z. mays基因型Z. mays c.v的Ubi-1启动子的基因调控元件在基因调控启动子元件,构建体和用于在植物细胞和/或植物组织中表达转基因的方法。 LLN37。

    Novel Maize Ubiquitin Promoters
    5.
    发明申请
    Novel Maize Ubiquitin Promoters 有权
    新型玉米泛素启动子

    公开(公告)号:US20150184176A1

    公开(公告)日:2015-07-02

    申请号:US14587762

    申请日:2014-12-31

    CPC classification number: C12N15/8216 C12N15/10

    Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v2.

    Abstract translation: 玉米罐头 B73泛素-1(Z. mays c.v.B73 Ubi-1)启动子驱动植物中高水平的组成型转基因表达。 重复使用相同的Z. mays c.v. 多基因构建体中的B73 Ubi-1启动子也可导致基因沉默,从而使得转基因产品的效力较差。 本发明提供了使用来自不同玉蜀黍属Z. luxurians v2的Ubi-1启动子的基因调控元件在植物细胞和/或植物组织中表达转基因的基因调控启动子元件,构建体和方法。

    ALTERED FAD2 AND FAD3 GENES IN BRASSICA AND THE MOLECULAR MARKER ASSISTED DETECTION THEREOF
    8.
    发明申请
    ALTERED FAD2 AND FAD3 GENES IN BRASSICA AND THE MOLECULAR MARKER ASSISTED DETECTION THEREOF 有权
    改良的FAD2和FAD3基因在BRASSICA和分子标记辅助检测

    公开(公告)号:US20150282445A1

    公开(公告)日:2015-10-08

    申请号:US14614284

    申请日:2015-02-04

    Abstract: The present invention provides methods of marker-assisted selection for high oleic/low linolenic traits in canola and in other oil seed crop species, as well as isolated nucleic acids for use as molecular markers in such methods. In particular, molecular markers and Brassica nucleic acid corresponding to fad2 and fad3 gene mutations are disclosed. The markers of the present invention are highly useful for the direct selection of desirable fad2 and fad3 alleles during marker-assisted trait introgression and breeding. In one aspect of the embodiment, two single nucleotide polymorphism (SNP) markers are provided that correspond to the alleles. Thus, the present invention advantageously permits one of skill in the art to breed for the molecular markers described herein, or derivatives thereof, rather than breeding for a high oleic/low linolenic phenotype.

    Abstract translation: 本发明提供了油菜和其他油料种子作物中高油酸/低亚麻酸性状的标记辅助选择方法,以及分离的核酸,用作这些方法中的分子标记。 特别地,公开了对应于fad2和fad3基因突变的分子标记和芸苔属核酸。 本发明的标记对于在标记辅助性状渗入和育种期间直接选择期望的fad2和fad3等位基因是非常有用的。 在该实施方案的一个方面,提供了对应于等位基因的两个单核苷酸多态性(SNP)标记。 因此,本发明有利地允许本领域技术人员繁殖本文所述的分子标记或其衍生物,而不是育种高油酸/低亚麻酸表型。

    Novel Maize Ubiquitin Promoters
    9.
    发明申请
    Novel Maize Ubiquitin Promoters 审中-公开
    新型玉米泛素启动子

    公开(公告)号:US20150184175A1

    公开(公告)日:2015-07-02

    申请号:US14587735

    申请日:2014-12-31

    CPC classification number: C12N15/8216 C12N15/10

    Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. B104.

    Abstract translation: 玉米罐头 B73泛素-1(Z. mays c.v.B73 Ubi-1)启动子驱动植物中高水平的组成型转基因表达。 重复使用相同的Z. mays c.v. 多基因构建体中的B73 Ubi-1启动子也可导致基因沉默,从而使得转基因产品的效力较差。 提供了使用来自不同Z. mays基因型Z. mays c.v的Ubi-1启动子的基因调控元件在基因调控启动子元件,构建体和用于在植物细胞和/或植物组织中表达转基因的方法。 B104。

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