公开(公告)号：US20190225947A1

公开(公告)日：2019-07-25

申请号：US16316988

申请日：2017-07-11

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter LOS ,  Rene Marcel DE JONG ,  Remko Tsjibbe WINTER ,  Ben DEN DULK

IPC: C12N9/04 ,  C12P7/46 ,  C12R1/865

Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.

公开(公告)号：US20160319245A1

公开(公告)日：2016-11-03

申请号：US15103237

申请日：2014-12-12

Applicant: DSM IP ASSETS B.V.

Inventor： René Marcel DE JONG ,  Zheng ZHAO ,  Ben DEN DULK ,  Remko Tsjibbe WINTER

IPC: C12N9/02 ,  C12P7/46

CPC classification number: C12N9/001 ,  C12P7/46 ,  C12Y103/01006

Abstract: The present invention relates to a variant polypeptide having fumarate reductase activity, which has modified NADP(H)-dependent and/or NAD(H)-dependent activity as compared with a reference polypeptide having fumarate reductase activity. Such a variant may be overexpressed in a host cell in order to improve production of a dicarboxylic acid.

Abstract translation: 本发明涉及具有富马酸酯还原酶活性的变体多肽，其与具有富马酸盐还原酶活性的参照多肽相比具有修饰的NADP（H） - 依赖和/或NAD（H） - 依赖性活性。 这种变体可以在宿主细胞中过表达，以改善二羧酸的产生。