公开(公告)号：US20190225947A1

公开(公告)日：2019-07-25

申请号：US16316988

申请日：2017-07-11

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter LOS ,  Rene Marcel DE JONG ,  Remko Tsjibbe WINTER ,  Ben DEN DULK

IPC: C12N9/04 ,  C12P7/46 ,  C12R1/865

Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.

公开(公告)号：US20170306377A1

公开(公告)日：2017-10-26

申请号：US15516854

申请日：2015-10-08

Applicant: DSM IP ASSETS B.V.

Inventor： Marco Alexander VAN DEN BERG ,  Marcella Katharina MADERN ,  Remko Tsjibbe WINTER

IPC: C12P19/56 ,  C12N15/52 ,  A23L27/30

CPC classification number: C12P19/56 ,  A23L27/33 ,  A23L27/36 ,  A23V2002/00 ,  C12N15/52 ,  C12P19/18 ,  C12Y106/02004 ,  C12Y114/13078 ,  C12Y114/13079 ,  C12Y204/00 ,  C12Y402/03019 ,  C12Y505/01013

Abstract: The present invention relates to a method for increasing the degree of glycosylation of a composition comprising steviol glycosides, which method comprises:a. contacting said composition comprising steviol glycosides with a recombinant microorganism, a cell free extract derived from such a microorganism or an enzyme preparation derived from either thereof; and b. thereby to increase the degree of glycosylation of the composition comprising steviol glycosides, wherein the recombinant microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity;a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; a polypeptide having kaurenoic acid 13-hydroxylase activity; and one or more polypeptides having UDP-glucosyltransferase activity whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one steviol glycoside. The present invention also relates to a composition comprising steviol glycosides obtainable by such a method.

公开(公告)号：US20160319245A1

公开(公告)日：2016-11-03

申请号：US15103237

申请日：2014-12-12

Applicant: DSM IP ASSETS B.V.

Inventor： René Marcel DE JONG ,  Zheng ZHAO ,  Ben DEN DULK ,  Remko Tsjibbe WINTER

IPC: C12N9/02 ,  C12P7/46

CPC classification number: C12N9/001 ,  C12P7/46 ,  C12Y103/01006

Abstract: The present invention relates to a variant polypeptide having fumarate reductase activity, which has modified NADP(H)-dependent and/or NAD(H)-dependent activity as compared with a reference polypeptide having fumarate reductase activity. Such a variant may be overexpressed in a host cell in order to improve production of a dicarboxylic acid.

Abstract translation: 本发明涉及具有富马酸酯还原酶活性的变体多肽，其与具有富马酸盐还原酶活性的参照多肽相比具有修饰的NADP（H） - 依赖和/或NAD（H） - 依赖性活性。 这种变体可以在宿主细胞中过表达，以改善二羧酸的产生。