公开(公告)号：US10000537B2

公开(公告)日：2018-06-19

申请号：US14895742

申请日：2014-06-04

Applicant: DSM IP ASSETS B.V.

Inventor： Paul Klaassen ,  Paulus Petrus De Waal ,  Rene Marcel De Jong ,  Arnold Jacob Mattieu Driessen ,  Jeroen Gerben Nijland ,  Hyun Yong Shin

IPC: C12P21/06 ,  C12P19/34 ,  C12N15/00 ,  C12N1/00 ,  C07H21/04 ,  C07K1/00 ,  C07K14/395 ,  C12P19/02

CPC classification number: C07K14/395 ,  C12P19/02

Abstract: The invention relates to a polypeptide having one or more substitution at a position corresponding to position 339 or 376 of SEQ ID NO: 59, wherein the polypeptide is a member of the Major Facilitator Superfamily (MFS). In an embodiment, the substitution is at position corresponding to 376 and wherein the amino acid at that position is replaced by an amino acid that has a van der Waals volume of 80 to 138 Å3 and a side chain hydrophobicity of 10 to 100 ΔtR.