公开(公告)号：US10881099B2

公开(公告)日：2021-01-05

申请号：US16316637

申请日：2017-07-06

Applicant: DSM IP Assets B.V.

Inventor： Manoj Kumar

IPC: A01N25/02 ,  A01N43/90

Abstract: The present invention relates to a process to improve the yield of strawberry crops, the process comprising the step of applying a polyene antifungal agent, especially natamycin, to the roots of a strawberry plant prior to planting said plant and/or to the medium to which the plant is to be planted. The claimed process results in an increased number of crop, an increase in the weight of individual crop, and an increase in the total weight of the crop, and may allow for an earlier harvest.

公开(公告)号：US20150031868A1

公开(公告)日：2015-01-29

申请号：US14373571

申请日：2013-01-23

Applicant: DSM IP ASSETS B.V.

Inventor： Martin Lehmann ,  Joshua Trueheart ,  Priscilla Zwart Jens ,  Liang Wu ,  Viktor Marius Boer ,  Cornelis Maria Jacobus Sagt ,  Manoj Kumar ,  Bernard Meijrink ,  Marco Alexander Van Den Berg

IPC: C12P19/56 ,  C12N9/10 ,  A23L1/236 ,  C07H15/256

CPC classification number: C12P19/56 ,  A23K20/10 ,  A23K20/121 ,  A23K20/163 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C07H15/256 ,  C12N9/0073 ,  C12N9/1051 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/007 ,  C12Y114/13078 ,  C12Y204/01035 ,  C12Y402/03019 ,  C12Y505/01013

Abstract: The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides.

Abstract translation: 本发明涉及包含一个或多个核苷酸序列的重组微生物，所述核苷酸序列编码：具有入 - copalyl焦磷酸合成酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有海藻酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力。 重组微生物还可能能够表达一种或多种UDP-葡糖基转移酶，使得微生物能够产生一种或多种甜菊糖苷。