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公开(公告)号：US11136600B2
公开(公告)日：2021-10-05
申请号：US15766184
申请日：2016-10-03
Applicant: DSM IP Assets B.V.
Inventor： Ioannis Papapetridis , Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Paul Klaassen
IPC: C12P7/02 , C12P7/10 , C12N1/18 , C12N9/00 , C12N9/04 , C12N9/02 , C12N9/12 , C12N9/18 , C12N15/52
Abstract: The present invention relates to a eukaryotic cell that is genetically modified comprising one or more heterologous gene encoding: a) D-glucose-6-phosphate dehydrogenase and/or b) 6-phosphogluconate dehydrogenase; and/or c) glucose dehydrogenase, gluconolactonase and gluconate kinase, wherein a), b) and glucose dehydrogenase in c) are NAD+ dependent.
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公开(公告)号：US09528117B2
公开(公告)日：2016-12-27
申请号：US14189989
申请日：2014-02-25
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
CPC classification number: C12N15/815 , C12N1/18 , C12N9/0006 , C12N9/0008 , C12N9/93 , C12P7/06 , C12P7/10 , C12Y101/01001 , C12Y101/01008 , C12Y102/0101 , C12Y602/01001 , Y02E50/16 , Y02E50/17 , Y02P20/52
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
Abstract translation: 本发明涉及酵母细胞，特别是重组酵母细胞，NADH依赖性甘油合成所需的缺乏酶活性的细胞或相对于NADH依赖性甘油合成具有降低的酶活性的细胞与其相应的 野生型酵母细胞，该细胞包含编码NAD +依赖性乙酰化乙醛脱氢酶（EC 1.2.1.10）活性的一种或多种异源核酸序列。 本发明还涉及根据本发明的细胞在制备乙醇中的用途。
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公开(公告)号：US10883110B2
公开(公告)日：2021-01-05
申请号：US16893065
申请日：2020-06-04
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US10533181B2
公开(公告)日：2020-01-14
申请号：US16409197
申请日：2019-05-10
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US11214810B2
公开(公告)日：2022-01-04
申请号：US16833342
申请日：2020-03-27
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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Patent Agency Ranking
公开(公告)号：US11198847B2
公开(公告)日：2021-12-14
申请号：US16966741
申请日：2019-01-31
Applicant: DSM IP ASSETS B.V.
Inventor： Antonius Jeroen Adriaan Van Maris , Jacobus Thomas Pronk , Maarten Dirk Verhoeven , Ioannis Papapetridis
Abstract: The invention relates to a method for preparing a yeast which is capable of simultaneously fermenting a pentose and a hexose sugar, the method comprising providing a yeast which comprises: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway, a disruption of a gene encoding a ribulose-phosphate 3-epimerase and a disruption of a gene encoding a glucose-6-phosphate isomerase, and one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and subjecting the yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate when grown on a media comprising a hexose and at least one pentose sugar, so as to obtain an evolved yeast.
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公开(公告)号：US11186850B2
公开(公告)日：2021-11-30
申请号：US16858163
申请日：2020-04-24
Applicant: DSM IP ASSETS B.V.
Inventor： Ioannis Papapetridis , Antonius Jeroen Adriaan Van Maris , Jacobus Thomas Pronk , Paulus Petrus De Waal , Niels Lauret , Maarten Dirk Verhoeven , Jozef Petrus Johannes Schmitz
Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.
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公开(公告)号：US20190264217A1
公开(公告)日：2019-08-29
申请号：US16409197
申请日：2019-05-10
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US11326175B2
公开(公告)日：2022-05-10
申请号：US17176073
申请日：2021-02-15
Applicant: DSM IP Assets B.V.
Inventor： Jacobus Thomas Pronk , Antonius Jeroen Adriaan Van Maris , Victor Gabriel Guadalupe Medina
Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
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公开(公告)号：US11326172B2
公开(公告)日：2022-05-10
申请号：US16763394
申请日：2018-11-12
Applicant: DSM IP ASSETS B.V.
Abstract: The invention relates to a variant of a parent polypeptide which is preferably a hexose transporter, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of SEQ ID NO: 1, comprises a substitution of amino acids N376 and T89, the positions of said amino acids being defined with reference to the amino acid sequence of SEQ ID NO: 1. Said polypeptide is suitable in the production of ethanol from arabinose-rich biomass such as corn stover and corn starch.
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