公开(公告)号：US11136600B2

公开(公告)日：2021-10-05

申请号：US15766184

申请日：2016-10-03

Applicant: DSM IP Assets B.V.

Inventor： Ioannis Papapetridis ,  Jacobus Thomas Pronk ,  Antonius Jeroen Adriaan Van Maris ,  Paul Klaassen

IPC: C12P7/02 ,  C12P7/10 ,  C12N1/18 ,  C12N9/00 ,  C12N9/04 ,  C12N9/02 ,  C12N9/12 ,  C12N9/18 ,  C12N15/52

Abstract: The present invention relates to a eukaryotic cell that is genetically modified comprising one or more heterologous gene encoding: a) D-glucose-6-phosphate dehydrogenase and/or b) 6-phosphogluconate dehydrogenase; and/or c) glucose dehydrogenase, gluconolactonase and gluconate kinase, wherein a), b) and glucose dehydrogenase in c) are NAD+ dependent.

公开(公告)号：US10689670B2

公开(公告)日：2020-06-23

申请号：US16309422

申请日：2017-06-13

Applicant: DSM IP ASSETS B.V.

Inventor： Ioannis Papapetridis ,  Antonius Jeroen Adriaan Van Maris ,  Jacobus Thomas Pronk ,  Paulus Petrus De Waal ,  Niels Lauret ,  Maarten Dirk Verhoeven ,  Jozef Petrus Johannes Schmitz

IPC: C12P7/06 ,  C12N15/81 ,  C12N1/16 ,  C12R1/645 ,  C12N9/12 ,  C12N9/88

Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.

公开(公告)号：US11667886B2

公开(公告)日：2023-06-06

申请号：US16621572

申请日：2018-06-04

Applicant: DSM IP Assets B.V.

Inventor： Ioannis Papapetridis ,  Jacobus Thomas Pronk

IPC: C12N1/16 ,  C12N15/52 ,  C12P7/10 ,  C12N1/18 ,  C12R1/865

CPC classification number: C12N1/16 ,  C12N1/185 ,  C12N15/52 ,  C12P7/10 ,  C12P2203/00 ,  C12R2001/865 ,  C12Y101/01008 ,  C12Y202/01001 ,  C12Y202/01002 ,  C12Y207/01019 ,  C12Y401/01039 ,  C12Y501/03001 ,  C12Y503/01006

Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein one or more genes of the non-oxidative branch of the pentose phosphate pathway are overexpressed and/or wherein said yeast cell comprises a deletion or disruption of a glycerol-3-phosphate dehydrogenase (GPD) gene.

公开(公告)号：US10913928B2

公开(公告)日：2021-02-09

申请号：US16471367

申请日：2017-12-18

Applicant: DSM IP Assets B.V.

Inventor： Ioannis Papapetridis ,  Antonius Jeroen Adriaan Van Maris ,  Jacobus Thomas Pronk

IPC: C12N1/18 ,  C12N9/00 ,  C12P7/10 ,  C12N9/04 ,  C12N9/02

Abstract: This invention relates to a recombinant cell, preferably a recombinant yeast cell comprising: a) a gene coding for an enzyme having glycerol-3-phosphate dehydrogenase activity, wherein said enzyme has a cofactor dependency for at least NADP+ and/or for NADPH; b) a gene encoding an enzyme having at least NAD+ dependent acetylating acetaldehyde dehydrogenase activity (EC 1.2.1.10); and c) a mutation or disruption in at least one gene selected from the group of GPD1 and GPD2. Said cell is suitable for ethanol production, has a reduced glycerol production at high ethanol yield.

公开(公告)号：US11198847B2

公开(公告)日：2021-12-14

申请号：US16966741

申请日：2019-01-31

Applicant: DSM IP ASSETS B.V.

Inventor： Antonius Jeroen Adriaan Van Maris ,  Jacobus Thomas Pronk ,  Maarten Dirk Verhoeven ,  Ioannis Papapetridis

IPC: C12N15/52 ,  C12N1/18 ,  C12N1/22 ,  C12N9/04 ,  C12N9/12 ,  C12N9/90 ,  C12N9/92 ,  C12P1/02

Abstract: The invention relates to a method for preparing a yeast which is capable of simultaneously fermenting a pentose and a hexose sugar, the method comprising providing a yeast which comprises: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway, a disruption of a gene encoding a ribulose-phosphate 3-epimerase and a disruption of a gene encoding a glucose-6-phosphate isomerase, and one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and subjecting the yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate when grown on a media comprising a hexose and at least one pentose sugar, so as to obtain an evolved yeast.

公开(公告)号：US11186850B2

公开(公告)日：2021-11-30

申请号：US16858163

申请日：2020-04-24

Applicant: DSM IP ASSETS B.V.

Inventor： Ioannis Papapetridis ,  Antonius Jeroen Adriaan Van Maris ,  Jacobus Thomas Pronk ,  Paulus Petrus De Waal ,  Niels Lauret ,  Maarten Dirk Verhoeven ,  Jozef Petrus Johannes Schmitz

IPC: C12P7/06 ,  C12N9/12 ,  C12N9/88 ,  C12N15/81 ,  C12N1/14 ,  C12N1/16 ,  C12R1/645

Abstract: The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.