公开(公告)号：US11655267B2

公开(公告)日：2023-05-23

申请号：US17292119

申请日：2019-11-08

Applicant: DSM IP Assets B.V.

Inventor： Manuela Baur ,  Estel Canet-Martinez ,  Ruediger Hainz ,  Ulrich Hoeller ,  Lisa Ann Laprade ,  John Michael Geremia ,  Ghislain Schyns

IPC: C07H1/00 ,  C07H3/06

CPC classification number: C07H1/00 ,  C07H3/06

Abstract: The present disclosure relates oligosaccharide preparations suitable for use in nutritional compositions such as animal feed and methods of manufacturing the described oligosaccharide preparations. The present disclosure further relates to nutritional compositions that comprise the oligosaccharide preparations. The disclosed oligosaccharide preparations can be advantageous as an animal feed due to, at least in part, their prebiotic utility and the presence of anhydro-subunit containing oligosaccharides, which can be used to detect and/or determine the presence and content of the disclosed oligosaccharide preparations in the nutritional composition.

公开(公告)号：US11911405B2

公开(公告)日：2024-02-27

申请号：US17292132

申请日：2019-11-08

Applicant: DSM IP Assets B.V.

Inventor： Stephane Duval ,  John Michael Geremia ,  Lisa Ann Laprade ,  April Waguespack-Levy ,  Estefania Perez Calvo ,  Ghislain Schyns ,  Viviane Verlhac ,  Joshua Thomas Claypool

IPC: A61K31/702 ,  A23K20/163 ,  A23K50/30 ,  A23K50/75

CPC classification number: A61K31/702 ,  A23K20/163 ,  A23K50/30 ,  A23K50/75

Abstract: The present disclosure relates to methods of feeding animals by providing feed additives that modulate the gut microbiome to improve the health, nutrition, and growth performance. The present disclosure further relates to methods of modulating the microbial species present in the gastrointestinal tract of an animal. Such modulation includes, for example, modulating the level or function of microbial species.

公开(公告)号：US20140315278A1

公开(公告)日：2014-10-23

申请号：US14225787

申请日：2014-03-26

Applicant: DSM IP Assets B.V.

Inventor： Adriano O. HENRIQUES ,  Ghislain Schyns ,  Thibaut José Wenzel ,  Sebastien Potot

IPC: C12N15/75

CPC classification number: C12N15/75 ,  A23K10/16 ,  A23K10/18 ,  A23K20/189 ,  A61K38/00 ,  C07K14/32 ,  C07K14/33 ,  C07K14/38 ,  C12N9/16 ,  C12Y301/03001 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y302/01031

Abstract: This invention discloses novel bacterial spore systems. Genetically modified or genetically engineered viable spore systems expressing bioactive polypeptides, for example bacteriocins and/or enzymatically active feed enzymes, at the spore surface, have a great potential use in animal feeding. Genetically modified or “genetically engineered inert spore systems expressing affinity ligands or immobilized enzymes at the surface have a great potential use in biocatalysis and in the construction of biocatalytic films. Especially the resistance to harsh chemicals, desiccation, strong pressure, or high temperatures allows the spores to be a potentially valuable tool for the display of bioactive molecules, like biocatalytic enzymes or bioactive feed enzymes that must survive harsh conditions to deliver their full potential. Finally, instead of translational fusions to spore structural genes, passenger bioactive polypeptides, as for example enzymes, bacteriocins, affinity ligands, can also be fused to spore-specific surface enzymes, for example to spore specific enzymes as mentioned herein above.

Abstract translation: 本发明公开了新颖的细菌孢子体系。 在孢子表面上表达生物活性多肽（例如细菌素和/或酶促活性饲料酶）的遗传修饰或遗传工程可行的孢子体系在动物饲养中具有巨大的潜在用途。 在表面上表达亲和配体或固定化酶的遗传修饰或“基因工程惰性孢子体系在生物催化和生物催化膜的构建中具有很大的潜在用途。 特别是对苛刻化学品，干燥，强压或高温的抵抗能使孢子成为显示生物活性分子（如生物催化酶或生物活性饲料酶）的潜在有价值的工具，生物活性分子必须经受恶劣条件才能发挥其潜力。 最后，代替熔解结构基因的翻译融合，乘客生物活性多肽（例如酶，细菌素，亲和配体）也可以与孢子特异性表面酶融合，例如如上文所述的孢子特异性酶。