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公开(公告)号:US11578292B2
公开(公告)日:2023-02-14
申请号:US17040771
申请日:2019-03-26
Applicant: DSM IP ASSETS B.V.
Abstract: The present invention relates to a process for reducing haze in a beverage comprising adding a protein arginine deiminase and a proline-specific endoprotease during preparation of the beverage. The invention further relates to a wort or beer comprising a protein arginine deiminase and a proline-specific endoprotease.
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公开(公告)号:US10913938B2
公开(公告)日:2021-02-09
申请号:US16318776
申请日:2017-07-27
Applicant: DSM IP ASSETS B.V.
Abstract: The invention relates to a polypeptide having cellulolytic enhancing activity, wherein the polypeptide is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 60% sequence identity with the amino acid sequence of SEQ ID NO: 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44 and/or 47; (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 60% sequence identity to the nucleotide sequence of SEQ ID NO: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 and/or 48; (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which hybridises under at least high stringency conditions with the complementary strand of SEQ ID NO: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 and/or 48; and (d) a fragment of the polypeptide of (a), (b), or (c), that has cellulolytic enhancing activity.
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公开(公告)号:US11193145B2
公开(公告)日:2021-12-07
申请号:US17041954
申请日:2019-03-27
Applicant: DSM IP ASSETS B.V.
Inventor: Maaike Appeldoorn , Loes Elizabeth Bevers
Abstract: The invention relates to an enzyme composition, a process for the preparation thereof and the use of the enzyme composition in enzymatic hydrolysis.
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公开(公告)号:US10337040B2
公开(公告)日:2019-07-02
申请号:US15307241
申请日:2015-04-29
Applicant: DSM IP Assets B.V.
Inventor: Bertus Noordam , Loes Elizabeth Bevers , Rudy Francois Maria Jozef Parton , Michael Petrus Jozef Berkhout
Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pretreatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein the amounts of formed hydrolysed oxidation products at the end of the enzymatic hydrolysis by the oxidation by LPMO of the lignocellulosic material containing cellulose and/or cello-oligosaccharides is kept between 3 to 80 g/kg glucan present in the lignocellulosic material by adding a suitable amount of oxygen after the pre-treatment and before and/or during the enzymatic hydrolysis to the lignocellulosic material, preferably the formed hydrolysed oxidation product is gluconic acid, an aldonic acid and/or geminal diol, more preferably the hydrolysed oxidation product is gluconic acid.
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公开(公告)号:US11499142B2
公开(公告)日:2022-11-15
申请号:US16463074
申请日:2017-11-23
Applicant: DSM IP Assets B.V.
Inventor: Loes Elizabeth Bevers , Maaike Appeldoorn , Margot Elisabeth Francoise Schooneveld-Bergmans , Herman Jan Pel
IPC: C12N9/00 , C12N9/42 , C12N9/02 , C12N9/26 , C12P19/00 , C12N9/24 , C12P7/10 , C12P19/02 , C12P19/14 , C12N9/18
Abstract: The application relates to an enzyme composition, a process for the preparation thereof and the use of the enzyme composition in enzymatic hydrolysis.
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6.
公开(公告)号:US20200270659A1
公开(公告)日:2020-08-27
申请号:US16408062
申请日:2019-05-09
Applicant: DSM IP ASSETS B.V.
Inventor: Bertus NOORDAM , Loes Elizabeth Bevers , Rudy Francois Maria Jozef Parton , Michael Petrus Jozef Berkhout
Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pretreatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein the amounts of formed hydrolysed oxidation products at the end of the enzymatic hydrolysis by the oxidation by LPMO of the lignocellulosic material containing cellulose and/or cello-oligosaccharides is kept between 3 to 80 g/kg glucan present in the lignocellulosic material by adding a suitable amount of oxygen after the pre-treatment and before and/or during the enzymatic hydrolysis to the lignocellulosic material, preferably the formed hydrolysed oxidation product is gluconic acid, an aldonic acid and/or geminal diol, more preferably the hydrolysed oxidation product is gluconic acid.
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公开(公告)号:US12152223B2
公开(公告)日:2024-11-26
申请号:US18152967
申请日:2023-01-11
Applicant: DSM IP ASSETS B.V.
Abstract: The present invention relates to a process for reducing haze in a beverage comprising adding a protein arginine deiminase and a proline-specific endoprotease during preparation of the beverage. The invention further relates to a wort or beer comprising a protein arginine deiminase and a proline-specific endoprotease.
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公开(公告)号:US10947573B2
公开(公告)日:2021-03-16
申请号:US16408062
申请日:2019-05-09
Applicant: DSM IP ASSETS B.V.
Inventor: Bertus Noordam , Loes Elizabeth Bevers , Rudy Francois Maria Jozef Parton , Michael Petrus Jozef Berkhout
Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pretreatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein the amounts of formed hydrolysed oxidation products at the end of the enzymatic hydrolysis by the oxidation by LPMO of the lignocellulosic material containing cellulose and/or cello-oligosaccharides is kept between 3 to 80 g/kg glucan present in the lignocellulosic material by adding a suitable amount of oxygen after the pre-treatment and before and/or during the enzymatic hydrolysis to the lignocellulosic material, preferably the formed hydrolysed oxidation product is gluconic acid, an aldonic acid and/or geminal diol, more preferably the hydrolysed oxidation product is gluconic acid.
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