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    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL
    2.
    发明申请
    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL 审中-公开
    木质纤维素材料的酶解水解方法

    公开(公告)号：US20150322471A1

    公开(公告)日：2015-11-12

    申请号：US14655349

    申请日：2014-01-09

    Applicant: DSM IP ASSETS B.V.

    Inventor： Johannes Petrus SMITS ,  Michael Petrus Jozef BERKHOUT ,  Bertus NOORDAM

    IPC: C12P19/14 ,  C12P19/02

    CPC classification number: C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12Y302/01004 ,  C13K1/02

    Abstract: The invention relates to a process for the hydrolysis of cellulose containing biomass which comprises a liquefaction step in which a first enzyme or first enzyme composition is added to liquefy at least part of the solids present in the biomass and to keep the viscosity of the cellulose containing biomass below 1000 cP, preferably below 800 cP, more preferably below 600 cP in the liquefaction step; followed by a saccharification step in which a second enzyme composition is added to form oligomeric and/or monomeric sugars; and whereby the first enzyme or first enzyme composition is different from the second enzyme composition; whereby the first enzyme or first enzyme composition comprises an endoglucanase; whereby the second enzyme composition comprises a cellulase; and whereby the first enzyme or first enzyme composition comprises more endogluconase than the second enzyme composition (expressed in protein wt %).

    Abstract translation: 本发明涉及一种水解含纤维素生物质的方法，其包括液化步骤，其中加入第一种酶或第一种酶组合物来液化存在于生物质中的至少部分固体并保持含有纤维素的粘度 生物质在液化步骤中低于1000cP，优选低于800cP，更优选低于600cP; 随后是糖化步骤，其中加入第二种酶组合物以形成低聚和/或单体糖; 并且由此所述第一酶或第一酶组合物不同于所述第二酶组合物; 由此第一酶或第一酶组合物包含内切葡聚糖酶; 由此第二酶组合物包含纤维素酶; 并且由此第一酶或第一酶组合物包含比第二酶组合物更多的内切葡聚糖酶（以蛋白质wt％表示）。

    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS
    5.
    发明申请
    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS 审中-公开

    公开(公告)号：US20180073048A1

    公开(公告)日：2018-03-15

    申请号：US15812978

    申请日：2017-11-14

    Applicant: DSM IP ASSETS B.V.

    Inventor： Bertus NOORDAM

    IPC: C12P19/14 ,  C12P19/02

    Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and e) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS
    6.
    发明申请
    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS 有权

    公开(公告)号：US20210180098A1

    公开(公告)日：2021-06-17

    申请号：US17170169

    申请日：2021-02-08

    Applicant: DSM IP ASSETS B.V.

    Inventor： Bertus NOORDAM ,  Loes Elizabeth BEVERS ,  Rudy Francois MariaJozef PARTON ,  Michael Petrus Jozef BERKHOUT

    IPC: C12P19/02 ,  C13K1/02 ,  D21C3/26 ,  C13K13/00 ,  C12P19/14

    Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pretreatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein the amounts of formed hydrolysed oxidation products at the end of the enzymatic hydrolysis by the oxidation by LPMO of the lignocellulosic material containing cellulose and/or cello-oligosaccharides is kept between 3 to 80 g/kg glucan present in the lignocellulosic material by adding a suitable amount of oxygen after the pre-treatment and before and/or during the enzymatic hydrolysis to the lignocellulosic material, preferably the formed hydrolysed oxidation product is gluconic acid, an aldonic acid and/or geminal diol, more preferably the hydrolysed oxidation product is gluconic acid.

    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS
    8.
    发明申请
    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS 审中-公开

    公开(公告)号：US20200270659A1

    公开(公告)日：2020-08-27

    申请号：US16408062

    申请日：2019-05-09

    Applicant: DSM IP ASSETS B.V.

    Inventor： Bertus NOORDAM ,  Loes Elizabeth Bevers ,  Rudy Francois Maria Jozef Parton ,  Michael Petrus Jozef Berkhout

    IPC: C12P19/02 ,  C13K1/02 ,  D21C3/26 ,  C13K13/00 ,  C12P19/14

    Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pretreatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein the amounts of formed hydrolysed oxidation products at the end of the enzymatic hydrolysis by the oxidation by LPMO of the lignocellulosic material containing cellulose and/or cello-oligosaccharides is kept between 3 to 80 g/kg glucan present in the lignocellulosic material by adding a suitable amount of oxygen after the pre-treatment and before and/or during the enzymatic hydrolysis to the lignocellulosic material, preferably the formed hydrolysed oxidation product is gluconic acid, an aldonic acid and/or geminal diol, more preferably the hydrolysed oxidation product is gluconic acid.

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