公开(公告)号：US20240279697A1

公开(公告)日：2024-08-22

申请号：US18561258

申请日：2022-05-17

申请人： DSM IP Assets B.V.

发明人： Manos PAPADAKIS

IPC分类号： C12P19/00 ,  C12N9/10 ,  C12N15/52

CPC分类号： C12P19/00 ,  C12N9/1051 ,  C12N15/52 ,  C12Y204/01038 ,  C12Y204/01122 ,  C12Y204/01147

摘要： This invention relates to a method of producing one or more human milk oligosaccharides (HMOs), in particular LNT and/or LNnT, in a genetically engineered cell comprising an enhanced oligosaccharide transport capability. The genetically modified cell comprises a series of genetic modification which enable the production of one or more HMO(s), and a series of genetic modification that enhances the transport of lactose and produced HMO(s).

公开(公告)号：US20240327886A1

公开(公告)日：2024-10-03

申请号：US18561233

申请日：2022-05-17

申请人： DSM IP Assets B.V.

发明人： Manos PAPADAKIS ,  Ted JOHANSON ,  Peter BECKER

IPC分类号： C12P19/04 ,  C07K14/245 ,  C12N1/20 ,  C12N9/10 ,  C12N15/52 ,  C12P19/18 ,  C12R1/19

CPC分类号： C12P19/04 ,  C07K14/245 ,  C12N1/20 ,  C12N9/1051 ,  C12N15/52 ,  C12P19/18 ,  C12R2001/19 ,  C12Y204/01062 ,  C12Y204/01069 ,  C12Y204/01222

摘要： This invention relates to a method of producing mixtures of various human milk oligosaccharides (HMOs) with unique HMO blend profiles, consisting predominantly of LNFP-I and LNT and of other HMOs in less significant amounts. The less abundant HMOs might be 2′-FL, LNT-II or DFL. The strategies for achieving specific HMO blends include strain engineering and fermentation methods.

公开(公告)号：US20240141307A1

公开(公告)日：2024-05-02

申请号：US18177070

申请日：2023-03-01

申请人： DSM IP Assets B.V.

发明人： Manos PAPADAKIS

IPC分类号： C12N9/10 ,  C07K14/24 ,  C12P19/04 ,  C12P19/18

CPC分类号： C12N9/1081 ,  C07K14/24 ,  C12P19/04 ,  C12P19/18 ,  C12Y204/99004

摘要： The present disclosure relates to the production of sialylated Human Milk Oligosaccharides (HMOs), in particular to the biosynthetic production of 3′-sialyllactose (3′SL), and to genetically engineered cells and methods suitable for said production.

公开(公告)号：US20240327885A1

公开(公告)日：2024-10-03

申请号：US18561123

申请日：2022-05-17

申请人： DSM IP Assets B.V.

发明人： Manos PAPADAKIS ,  Ted JOHANSON ,  Peter BECKER

IPC分类号： C12P19/00 ,  C12N9/10

CPC分类号： C12P19/00 ,  C12N9/1051 ,  C12Y204/01086

摘要： The present disclosure relates to a method for the production of a human milk oligosaccharide (HMO) blend with LNFP-I and 2′-FL as the predominant HMO's, the method comprising the steps of providing a genetically engineered cell, which comprises a heterologous β-1,3-N-acetyl-glucosaminyltransferase protein, a heterologous β-1,3-galactosyltransferase protein, a heterologous α-1,2-fucosyltransferase protein, and expresses functionally the colanic acid gene cluster, comprises a native or heterologous regulatory or episomal element for controlling the expression of the proteins and optionally express a heterologous sugar transporter, and culturing the cell in a suitable cell culture medium to express said proteins and to produce an HMO blend.