公开(公告)号：US20240327886A1

公开(公告)日：2024-10-03

申请号：US18561233

申请日：2022-05-17

Applicant: DSM IP Assets B.V.

Inventor： Manos PAPADAKIS ,  Ted JOHANSON ,  Peter BECKER

IPC: C12P19/04 ,  C07K14/245 ,  C12N1/20 ,  C12N9/10 ,  C12N15/52 ,  C12P19/18 ,  C12R1/19

CPC classification number: C12P19/04 ,  C07K14/245 ,  C12N1/20 ,  C12N9/1051 ,  C12N15/52 ,  C12P19/18 ,  C12R2001/19 ,  C12Y204/01062 ,  C12Y204/01069 ,  C12Y204/01222

Abstract: This invention relates to a method of producing mixtures of various human milk oligosaccharides (HMOs) with unique HMO blend profiles, consisting predominantly of LNFP-I and LNT and of other HMOs in less significant amounts. The less abundant HMOs might be 2′-FL, LNT-II or DFL. The strategies for achieving specific HMO blends include strain engineering and fermentation methods.

公开(公告)号：US20240327777A1

公开(公告)日：2024-10-03

申请号：US18709000

申请日：2022-11-10

Applicant: DSM IP ASSETS B.V.

Inventor： Natalia CHRAPKOVA ,  Peter BECKER ,  Ted JOHANSON ,  Mikael JANSEN

IPC: C12M1/36 ,  C12M1/34

CPC classification number: C12M41/48 ,  C12M41/32 ,  C12M41/36

Abstract: A feed control system and related methods for a heterologous product, such as a human milk oligosaccharide, fermentation system are disclosed. The feed control system comprises a feed controller. The feed controller comprises a processor and an interface. The processor is configured to obtain a biomass content in a fermentation broth. The processor is configured to determine a carbon input parameter based on the biomass content. The processor is configured to control a carbon input to the fermentation broth according to the carbon input parameter.

公开(公告)号：US20240409972A1

公开(公告)日：2024-12-12

申请号：US18736354

申请日：2024-06-06

Applicant: DSM IP Assets B.V.

Inventor： Peter BECKER ,  Manos PAPADAKIS

IPC: C12P19/04 ,  C12N1/20 ,  C12R1/19

Abstract: The present disclosure relates to the production of oligosaccharides, especially Human milk Oligosaccharides (HMOs) using a genetically engineered cell which has decreased or total loss of function of phosphoglycerol transferase I and II and/or phosphoethanolamine transferase and/or glucans biosynthesis protein C to reduce oligosaccharide by-products and/or increase oligosaccharide production.

公开(公告)号：US20240327885A1

公开(公告)日：2024-10-03

申请号：US18561123

申请日：2022-05-17

Applicant: DSM IP Assets B.V.

Inventor： Manos PAPADAKIS ,  Ted JOHANSON ,  Peter BECKER

IPC: C12P19/00 ,  C12N9/10

CPC classification number: C12P19/00 ,  C12N9/1051 ,  C12Y204/01086

Abstract: The present disclosure relates to a method for the production of a human milk oligosaccharide (HMO) blend with LNFP-I and 2′-FL as the predominant HMO's, the method comprising the steps of providing a genetically engineered cell, which comprises a heterologous β-1,3-N-acetyl-glucosaminyltransferase protein, a heterologous β-1,3-galactosyltransferase protein, a heterologous α-1,2-fucosyltransferase protein, and expresses functionally the colanic acid gene cluster, comprises a native or heterologous regulatory or episomal element for controlling the expression of the proteins and optionally express a heterologous sugar transporter, and culturing the cell in a suitable cell culture medium to express said proteins and to produce an HMO blend.