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    Insect inhibitory proteins
    2.
    发明授权
    Insect inhibitory proteins 有权

    公开(公告)号：US11987800B2

    公开(公告)日：2024-05-21

    申请号：US17934287

    申请日：2022-09-22

    申请人： Monsanto Technology LLC

    发明人： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe

    IPC分类号： C12N15/82 ,  A01N63/50 ,  C07K14/24 ,  C07K14/325

    CPC分类号： C12N15/8286 ,  A01N63/50 ,  C07K14/24 ,  C07K14/325

    摘要： A pesticidal protein class of PirA, PirB, and PirAB fusion proteins exhibiting toxic activity against Coleopteran, Lepidopteran, and Hemipteran pest species is disclosed. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the PirA, PirB, and PirAB fusion proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran, Lepidopteran, and Hemipteran infestation are provided which contain recombinant nucleic acid sequences encoding the PirA, PirB, and PirAB fusion proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, and Hemipteran species pests using the PirA, PirB, and PirAB fusion proteins are also provided.

    PHI-4 Polypeptides and Methods For Their Use
    7.
    发明申请
    PHI-4 Polypeptides and Methods For Their Use 审中-公开
    PHI-4多肽及其使用方法

    公开(公告)号：US20160281105A1

    公开(公告)日：2016-09-29

    申请号：US15178330

    申请日：2016-06-09

    申请人： PIONEER HI-BRED INTERANTIONAL, INC.

    发明人： RUTH CONG ,  JINGTONG HOU ,  ZHENGLIN HOU ,  PHILLIP PATTEN ,  TAKASHI YAMAMOTO

    IPC分类号： C12N15/82 ,  C07K14/245 ,  C07K14/24 ,  C07K14/32 ,  A01N43/50 ,  C07K14/21

    CPC分类号： C12N15/8286 ,  A01N37/46 ,  A01N43/50 ,  C07K14/195 ,  C07K14/21 ,  C07K14/24 ,  C07K14/245 ,  C07K14/32 ,  C07K2319/02 ,  Y02A40/162

    摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.

    摘要翻译： 提供了控制害虫的组成和方法。 该方法涉及用编码杀虫蛋白的核酸序列转化生物体。 特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。 因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。 组合物是杀虫的核酸和细菌物种的蛋白质。 这些序列用于构建表达载体，用于随后转化为感兴趣的生物体，作为用于分离其他同源（或部分同源的）基因的探针。 杀虫蛋白用于控制，抑制生长或杀死鳞翅目，鞘翅目，dipteran，真菌，半翅目和线虫害虫种群，并用于生产具有杀虫活性的组合物。

    MICROORGANISM FOR METHIONINE PRODUCTION WITH IMPROVED METHIONINE SYNTHASE ACTIVITY AND METHIONINE EFFLUX
    8.
    发明申请
    MICROORGANISM FOR METHIONINE PRODUCTION WITH IMPROVED METHIONINE SYNTHASE ACTIVITY AND METHIONINE EFFLUX 审中-公开
    具有改善的甲硫氨酸合成酶活性和甲硫氨酸的甲磺酸生产微生物

    公开(公告)号：US20160177352A1

    公开(公告)日：2016-06-23

    申请号：US14915137

    申请日：2014-09-01

    申请人： METABOLIC EXPLORER

    发明人： Wanda DISCHERT ,  Perrine VASSEUR ,  Rainer FIGGE

    IPC分类号： C12P13/12 ,  C12N15/77 ,  C12N9/10 ,  C12N15/70

    CPC分类号： C12P13/12 ,  C07K14/24 ,  C07K14/245 ,  C07K14/34 ,  C12N9/1007 ,  C12N15/70 ,  C12N15/77 ,  C12Y201/01013

    摘要： The present application is related to a recombinant microorganism optimised for the fermentative production of methionine and/or its derivatives, wherein in said recombinant microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced. The application is also related to a method for optimizing the fermentative production of methionine and/or its derivatives comprising the steps of: c. culturing a recombinant microorganism wherein in said microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and d. recovering methionine and/or its derivatives from the culture medium.

    摘要翻译： 本申请涉及针对甲硫氨酸和/或其衍生物的发酵产生优化的重组微生物，其中在所述重组微生物中，增强了钴胺素依赖性甲硫氨酸合成酶活性和甲硫氨酸流出。 该应用还涉及优化甲硫氨酸和/或其衍生物的发酵生产的方法，包括以下步骤：c。 培养重组微生物，其中在包含可发酵碳源和硫源的适当培养基中，所述微生物中钴胺素依赖性甲硫氨酸合成酶活性和甲硫氨酸流出增强，d。 从培养基中回收甲硫氨酸和/或其衍生物。