公开(公告)号：US20240226307A9

公开(公告)日：2024-07-11

申请号：US18277915

申请日：2022-02-22

Applicant: Duke University

Inventor： Ashutosh Chilkoti ,  Bruce Sullenger ,  Imran Ozer ,  Angus Hucknall ,  Juliana Layzer ,  George Pitoc

IPC: A61K47/60 ,  A61K31/7088 ,  A61P7/02 ,  C12N15/115

CPC classification number: A61K47/60 ,  A61K31/7088 ,  A61P7/02 ,  C12N15/115

Abstract: Disclosed are POEGMA-aptamer conjugates with a reduced or eliminated host-immune response. An example conjugate includes an aptamer conjugated to a POEGMA having a plurality of side chains, where each side chain includes 1 to 6 monomers of ethylene glycol repeated in tandem. Also disclosed are methods of making the conjugate and methods of using the conjugate. An example method of use includes a method of controlling coagulation in a subject.

公开(公告)号：US20240131171A1

公开(公告)日：2024-04-25

申请号：US18277915

申请日：2022-02-22

Applicant: Duke University

Inventor： Ashutosh Chilkoti ,  Bruce Sullenger ,  Imran Ozer ,  Angus Hucknall ,  Juliana Layzer ,  George Pitoc

IPC: A61K47/60 ,  A61K31/7088 ,  A61P7/02 ,  C12N15/115

CPC classification number: A61K47/60 ,  A61K31/7088 ,  A61P7/02 ,  C12N15/115

Abstract: Disclosed are POEGMA-aptamer conjugates with a reduced or eliminated host-immune response. An example conjugate includes an aptamer conjugated to a POEGMA having a plurality of side chains, where each side chain includes 1 to 6 monomers of ethylene glycol repeated in tandem. Also disclosed are methods of making the conjugate and methods of using the conjugate. An example method of use includes a method of controlling coagulation in a subject.

公开(公告)号：US20210395754A1

公开(公告)日：2021-12-23

申请号：US17464474

申请日：2021-09-01

Applicant: Duke University

Inventor： Jennifer Freedman ,  Brendon Patierno ,  Bonnie LaCroix ,  Timothy Robinson ,  Bruce Sullenger ,  Daniel George ,  Steven Patierno

IPC: C12N15/113 ,  C07H21/04 ,  C12N15/11 ,  A61P35/00 ,  A61K31/7105 ,  A61K45/06

Abstract: The present disclosure provides methods and compositions for the treatment of cancer. In some aspects, the present disclosure provides splice-switching oligonucleotides that downregulate AR or EGFR expression and methods of using these splice-switching oligonucleotides to treat cancer.

公开(公告)号：US10889816B2

公开(公告)日：2021-01-12

申请号：US16334307

申请日：2017-09-18

Applicant: DUKE UNIVERSITY ,  OHIO STATE INNOVATION FOUNDATION

Inventor： Shahid M. Nimjee ,  George A. Pitoc ,  Juliana Layzer ,  Bruce Sullenger

IPC: A61K48/00 ,  C07H21/04 ,  C12N15/113 ,  C12N15/115 ,  A61K31/7115 ,  A61K47/64 ,  A61K45/06 ,  A61K47/60 ,  A61K31/7105 ,  A61K47/54 ,  A61P7/02 ,  A61K31/7088

Abstract: Provided herein are aptamers capable of inhibiting the activity of Von Willebrand Factor (VWF). Pharmaceutical compositions comprising these aptamers are also provided. Methods of preventing blood clot formation in a subject by administering the aptamers are provided and methods of treating a blood clot by administering a VWF-targeting agent are also provided.

公开(公告)号：US20240141430A1

公开(公告)日：2024-05-02

申请号：US18128949

申请日：2023-03-30

Applicant: DUKE UNIVERSITY

Inventor： Bruce Sullenger ,  Bethany Gray ,  Michael Nichols

IPC: C12Q1/6881 ,  C12N5/09 ,  C12N15/115

CPC classification number: C12Q1/6881 ,  C12N5/0693 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/3517

Abstract: Provided herein are compositions and methods for sorting and/or identifying live cells. The compositions and methods provide for staining of live cells with aptamer so particular cells can be identified within or sorted from a heterogeneous population of live cells and subsequent reversal of the staining to prepare sorted and/or identified cells in their native state.

公开(公告)号：US11634772B2

公开(公告)日：2023-04-25

申请号：US16496871

申请日：2018-03-23

Applicant: Duke University

Inventor： Bruce Sullenger ,  Bethany Gray ,  Michael Nichols

IPC: C12N15/115 ,  C12N5/09 ,  C12Q1/6881

Abstract: Provided herein are compositions and methods for sorting and/or identifying live cells. The compositions and methods provide for staining of live cells with aptamer so particular cells can be identified within or sorted from a heterogeneous population of live cells and subsequent reversal of the staining to prepare sorted and/or identified cells in their native state.

公开(公告)号：US11136584B2

公开(公告)日：2021-10-05

申请号：US15773841

申请日：2016-11-04

Applicant: Duke University

Inventor： Jennifer Freedman ,  Brendon Patierno ,  Bonnie Lacroix ,  Timothy Robinson ,  Bruce Sullenger ,  Daniel George ,  Steven Patierno

IPC: C12N15/11 ,  C12N15/113 ,  C07H21/04 ,  A61P35/00 ,  A61K31/7105 ,  A61K45/06 ,  A61K48/00

Abstract: The present disclosure provides methods and compositions for the treatment of cancer. In some aspects, the present disclosure provides splice-switching oligonucleotides that downregulate AR or EGFR expression and methods of using these splice-switching oligonucleotides to treat cancer.

公开(公告)号：US20250136995A1

公开(公告)日：2025-05-01

申请号：US18733806

申请日：2024-06-04

Applicant: Duke University

Inventor： Jennifer Freedman ,  Brendon Patierno ,  Bonnie LaCroix ,  Timothy Robinson ,  Bruce Sullenger ,  Daniel George ,  Steven Patierno

IPC: C12N15/113 ,  A61K31/7105 ,  A61K31/7125 ,  A61K45/06 ,  A61K48/00 ,  A61P35/00 ,  C07H21/04 ,  C12N15/11

Abstract: The present disclosure provides methods and compositions for the treatment of cancer. In some aspects, the present disclosure provides splice-switching oligonucleotides that downregulate AR or EGFR expression and methods of using these splice-switching oligonucleotides to treat cancer.

公开(公告)号：US11965160B2

公开(公告)日：2024-04-23

申请号：US17146147

申请日：2021-01-11

Applicant: Duke University ,  Ohio State Innovation Foundation

Inventor： Shahid M. Nimjee ,  Bruce Sullenger ,  George A. Pitoc ,  Juliana Layzer

IPC: C07H21/02 ,  A61K31/7088 ,  A61K31/7105 ,  A61K31/7115 ,  A61K45/06 ,  A61K47/54 ,  A61K47/60 ,  A61K47/64 ,  A61P7/02 ,  C07H21/04 ,  C12N15/113 ,  C12N15/115

CPC classification number: C12N15/113 ,  A61K31/7088 ,  A61K31/7105 ,  A61K31/7115 ,  A61K45/06 ,  A61K47/554 ,  A61K47/60 ,  A61K47/64 ,  A61P7/02 ,  C12N15/115 ,  C12N2310/113 ,  C12N2310/16 ,  C12N2310/317 ,  C12N2310/3183 ,  C12N2310/3515 ,  C12N2310/321 ,  C12N2310/3521 ,  C12N2310/322 ,  C12N2310/3533 ,  A61K31/7105 ,  A61K2300/00 ,  A61K31/7115 ,  A61K2300/00

Abstract: Provided herein are aptamers capable of inhibiting the activity of Von Willebrand Factor (VWF). Pharmaceutical compositions comprising these aptamers are also provided. Methods of preventing blood clot formation in a subject by administering the aptamers are provided and methods of treating a blood clot by administering a VWF-targeting agent are also provided.

公开(公告)号：US20230012024A1

公开(公告)日：2023-01-12

申请号：US17782848

申请日：2020-12-04

Applicant: Duke University

Inventor： Bruce Sullenger ,  Elisabeth Tracy ,  Christopher Reed

IPC: C12N15/115 ,  A61P7/02 ,  A61K47/60 ,  A61K47/54

Abstract: The present application provides for a method for controlling coagulation in a subject in need of extracorporeal membrane oxygenation. The method comprises administering an effective amount of an anticoagulant agent that directly inhibits one or more steps in a coagulation pathway to the subject, wherein the effective amount of the anticoagulant agent is capable of controlling coagulation during extra corporeal membrane oxygenation for a coagulation control time of at least 4 hours.