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1.发明授权Systems and methods for extracting lipids from and dehydrating wet algal biomass 失效从湿藻类生物质提取脂质和脱水的系统和方法公开(公告)号：US08765983B2
公开(公告)日：2014-07-01
申请号：US12983767
申请日：2011-01-03
申请人： Daniel Fleischer , Marko Jukic , Andrew Thompson , Guido Radaelli
发明人： Daniel Fleischer , Marko Jukic , Andrew Thompson , Guido Radaelli
IPC分类号： C11B1/00
摘要： Exemplary methods include centrifuging a wet algal biomass to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a centrifuged algal biomass, mixing the centrifuged algal biomass with an amphiphilic solvent to result in a mixture, heating the mixture to result in a dehydrated, defatted algal biomass, separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids, evaporating the amphiphilic solvent from the water and the lipids, and separating the water from the lipids. The amphiphilic solvent may be selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde. Other exemplary methods include filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass.
摘要翻译： 示例性方法包括将湿藻类生物质离心以将湿藻类生物质的固体含量提高到约10％至40％之间，以产生离心的藻类生物质，将离心的藻类生物质与两亲溶剂混合以产生混合物，加热 混合物导致脱水的脱脂藻类生物质，将两亲溶剂与脱水的脱脂藻类生物质分离以产生两亲溶剂，水和脂质，从水和脂质中蒸发出两亲溶剂，并将水从 脂质 两亲溶剂可以选自丙酮，甲醇，乙醇，异丙醇，丁酮，二甲醚和丙醛。 其他示例性方法包括通过膜过滤湿藻类生物质以将湿藻类生物质的固体含量增加至约10％至40％之间，以产生过滤的藻类生物质。
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2.发明授权Systems and methods for extracting lipids from and dehydrating wet algal biomass 有权从湿藻类生物质提取脂质和脱水的系统和方法公开(公告)号：US07868195B2
公开(公告)日：2011-01-11
申请号：US12610134
申请日：2009-10-30
申请人： Daniel Fleischer , Marko Jukic , Andrew Thompson , Guido Radaelli
发明人： Daniel Fleischer , Marko Jukic , Andrew Thompson , Guido Radaelli
IPC分类号： C11B1/00
摘要： Exemplary methods include centrifuging a wet algal biomass to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a centrifuged algal biomass, mixing the centrifuged algal biomass with an amphiphilic solvent to result in a mixture, heating the mixture to result in a dehydrated, defatted algal biomass, separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids, evaporating the amphiphilic solvent from the water and the lipids, and separating the water from the lipids. The amphiphilic solvent may be selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde. Other exemplary methods include filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass.
摘要翻译： 示例性方法包括将湿藻类生物质离心以将湿藻类生物质的固体含量提高到约10％至40％之间，以产生离心的藻类生物质，将离心的藻类生物质与两亲溶剂混合以产生混合物，加热 混合物导致脱水的脱脂藻类生物质，将两亲溶剂与脱水的脱脂藻类生物质分离以产生两亲溶剂，水和脂质，从水和脂质中蒸发出两亲溶剂，并将水从 脂质 两亲溶剂可以选自丙酮，甲醇，乙醇，异丙醇，丁酮，二甲醚和丙醛。 其他示例性方法包括通过膜过滤湿藻类生物质以将湿藻类生物质的固体含量增加至约10％至40％之间，以产生过滤的藻类生物质。
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公开(公告)号：US08747930B2
公开(公告)日：2014-06-10
申请号：US12494170
申请日：2009-06-29
申请人： Daniel Fleischer , Andrew Thompson , Marko Jukic , Guido Radaelli
发明人： Daniel Fleischer , Andrew Thompson , Marko Jukic , Guido Radaelli
IPC分类号： C12C3/00
CPC分类号： C12P7/6463 , C12N1/12
摘要： Various aspects provide for extracting siliceous particles. Siliceous particles may include or be derived from diatoms. Certain embodiments provide for segregating suspensions into two or more segregation products. In some cases, a first product includes siliceous particles, and a second product may include hydrophobic species. Certain aspects provide for extracting non-siliceous biomass (e.g., lipids).
摘要翻译： 各种方面提供硅质颗粒。 硅质颗粒可以包括或衍生自硅藻。 某些实施方案提供将悬浮液分离成两种或更多种分离产物。 在一些情况下，第一产品包括硅质颗粒，第二产品可以包括疏水物质。 某些方面提供非硅质生物质（例如脂质）的提取。
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公开(公告)号：US20110072713A1
公开(公告)日：2011-03-31
申请号：US12571229
申请日：2009-09-30
申请人： Daniel Fleischer , Andrew Thompson , Marko Jukic , Guido Radaelli , Jon Marshall
发明人： Daniel Fleischer , Andrew Thompson , Marko Jukic , Guido Radaelli , Jon Marshall
摘要： A method for converting lipids to alkyl esters may include receiving a reactant comprising one or more lipids. In some cases, the reactant may include substantial amounts of polar lipids and/or free fatty acids. Some reactants may be derived from photosynthetic organisms, such as algae and/or diatoms. The reactant may be mixed with an alcohol and a catalyst to form a mixture. The mixture may be heated, for example, to a temperature between 50 and 350 degrees Celsius, including between 80 and 220 degrees Celsius. Pressure may be controlled to be between 1 and 200 bar, including between 10 and 100 bar. At least a portion of the reactant may be converted to one or more alkyl esters. A biofuel may include alkyl esters made from lipids according to various methods.
摘要翻译： 将脂质转化为烷基酯的方法可包括接收包含一种或多种脂质的反应物。 在一些情况下，反应物可以包括大量的极性脂质和/或游离脂肪酸。 一些反应物可以衍生自光合生物，例如藻类和/或硅藻。 反应物可以与醇和催化剂混合以形成混合物。 混合物可以被加热到例如介于50和350摄氏度之间的温度，包括在80和220摄氏度之间。 压力可以控制在1至200巴之间，包括10至100巴之间。 反应物的至少一部分可以转化成一种或多种烷基酯。 生物燃料可以包括根据各种方法由脂质制成的烷基酯。
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公开(公告)号：US20100330658A1
公开(公告)日：2010-12-30
申请号：US12494170
申请日：2009-06-29
申请人： Daniel Fleischer , Andrew Thompson , Marko Jukic , Guido Radaelli
发明人： Daniel Fleischer , Andrew Thompson , Marko Jukic , Guido Radaelli
CPC分类号： C12P7/6463 , C12N1/12
摘要： Various aspects provide for extracting siliceous particles. Siliceous particles may include or be derived from diatoms. Certain embodiments provide for segregating suspensions into two or more segregation products. In some cases, a first product includes siliceous particles, and a second product may include hydrophobic species. Certain aspects provide for extracting non-siliceous biomass (e.g., lipids).
摘要翻译： 各种方面提供硅质颗粒。 硅质颗粒可以包括或衍生自硅藻。 某些实施方案提供将悬浮液分离成两种或更多种分离产物。 在一些情况下，第一产品包括硅质颗粒，第二产品可以包括疏水物质。 某些方面提供非硅质生物质（例如脂质）的提取。
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6.发明申请Systems and Methods for Extracting Lipids from and Dehydrating Wet Algal Biomass 失效用于从湿藻类生物质中提取脂质和脱水的系统和方法公开(公告)号：US20110196163A1
公开(公告)日：2011-08-11
申请号：US12983767
申请日：2011-01-03
申请人： Daniel Fleischer , Marko Jukic , Andrew Thompson , Guido Radaelli
发明人： Daniel Fleischer , Marko Jukic , Andrew Thompson , Guido Radaelli
IPC分类号： C11B7/00
摘要： Exemplary methods include centrifuging a wet algal biomass to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a centrifuged algal biomass, mixing the centrifuged algal biomass with an amphiphilic solvent to result in a mixture, heating the mixture to result in a dehydrated, defatted algal biomass, separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids, evaporating the amphiphilic solvent from the water and the lipids, and separating the water from the lipids. The amphiphilic solvent may be selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde. Other exemplary methods include filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass.
摘要翻译： 示例性方法包括将湿藻类生物质离心以将湿藻类生物质的固体含量提高到约10％至40％之间，以产生离心的藻类生物质，将离心的藻类生物质与两亲溶剂混合以产生混合物，加热 混合物导致脱水的脱脂藻类生物质，将两亲溶剂与脱水的脱脂藻类生物质分离以产生两亲溶剂，水和脂质，从水和脂质中蒸发出两亲溶剂，并将水从 脂质 两亲溶剂可以选自丙酮，甲醇，乙醇，异丙醇，丁酮，二甲醚和丙醛。 其他示例性方法包括通过膜过滤湿藻类生物质以将湿藻类生物质的固体含量增加至约10％至40％之间，以产生过滤的藻类生物质。
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公开(公告)号：US20110041386A1
公开(公告)日：2011-02-24
申请号：US12544079
申请日：2009-08-19
CPC分类号： C11B1/10 , B03D3/02 , C10G2300/1011 , C10L1/02 , C11B1/106 , C11B3/008 , C11B3/16 , C11C3/003 , Y02P30/20
摘要： A suspension may include an aqueous liquid and suspended particles. The particles may include a nonpolar and/or hydrophobic substance (e.g., a lipid) substantially contained within polar and/or hydrophilic exterior layers. A method for extracting the suspended lipids may include adding a nonpolar solvent to the suspension and disrupting the exterior layers to expose the lipids to the nonpolar solvent. In some cases, particles may also include interior hydrophilic portions (e.g., intracellular water), which may be exposed to the aqueous liquid via disruption of the exteriors. The mixture may be accelerated to segregate the mixture into first and second products. The first product may have a majority of the nonpolar and/or hydrophobic substances. The second product may have a majority of the polar substances.
摘要翻译： 悬浮液可以包括水性液体和悬浮颗粒。 颗粒可以包括基本上包含在极性和/或亲水外层内的非极性和/或疏水性物质（例如脂质）。 用于提取悬浮的脂质的方法可以包括向悬浮液中加入非极性溶剂并破坏外层以将脂质暴露于非极性溶剂。 在一些情况下，颗粒还可以包括内部亲水部分（例如细胞内水），其可通过外部的破坏而暴露于含水液体。 可以加速混合物以将混合物分离成第一和第二产物。 第一种产品可能具有大部分非极性和/或疏水物质。 第二种产品可能具有大多数极性物质。
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8.发明申请Systems and Methods for Extracting Lipids from and Dehydrating Wet Algal Biomass 有权用于从湿藻类生物质中提取脂质和脱水的系统和方法公开(公告)号：US20100261922A1
公开(公告)日：2010-10-14
申请号：US12610134
申请日：2009-10-30
申请人： Daniel Fleischer , Marko Jukic , Andrew Thompson , Guido Radaelli
发明人： Daniel Fleischer , Marko Jukic , Andrew Thompson , Guido Radaelli
IPC分类号： C11B7/00
摘要： Exemplary methods include centrifuging a wet algal biomass to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a centrifuged algal biomass, mixing the centrifuged algal biomass with an amphiphilic solvent to result in a mixture, heating the mixture to result in a dehydrated, defatted algal biomass, separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids, evaporating the amphiphilic solvent from the water and the lipids, and separating the water from the lipids. The amphiphilic solvent may be selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde. Other exemplary methods include filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass.
摘要翻译： 示例性方法包括将湿藻类生物质离心以将湿藻类生物质的固体含量提高到约10％至40％之间，以产生离心的藻类生物质，将离心的藻类生物质与两亲溶剂混合以产生混合物，加热 混合物导致脱水的脱脂藻类生物质，将两亲溶剂与脱水的脱脂藻类生物质分离以产生两亲溶剂，水和脂质，从水和脂质中蒸发出两亲溶剂，并将水从 脂质 两亲溶剂可以选自丙酮，甲醇，乙醇，异丙醇，丁酮，二甲醚和丙醛。 其他示例性方法包括通过膜过滤湿藻类生物质以将湿藻类生物质的固体含量增加至约10％至40％之间，以产生过滤的藻类生物质。
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公开(公告)号：US20100317088A1
公开(公告)日：2010-12-16
申请号：US12485027
申请日：2009-06-15
申请人： Guido Radaelli , Daniel Fleischer
发明人： Guido Radaelli , Daniel Fleischer
CPC分类号： C12P7/6463 , C12M21/02 , C12M47/06 , C12N1/066
摘要： Presented herein are exemplary systems and methods for extracting lipids from a wet algal biomass. An exemplary method comprises lysing a wet algal biomass with an insoluble granular lysing agent to create a lysate, creating a lipid-rich phase in the lysate, and separating the lipid-rich phase from the lysate. An exemplary system comprises a lysing station for creating a lysate from a wet algal biomass and a separation station for creating and separating a lipid-rich phase from the lysate. According to further exemplary systems and methods, ultrasound may be used in place of or in addition to a lysing agent to lyse the wet algal biomass.
摘要翻译： 本文提出的是用于从湿藻类生物质中提取脂质的示例性系统和方法。 一个示例性的方法包括用不溶性颗粒状裂解剂裂解湿藻类生物质以产生裂解物，在裂解物中产生富含脂质的相，并将富脂相与裂解物分离。 示例性系统包括用于从湿藻类生物质产生裂解物的裂解站和用于从裂解物中产生和分离富脂相的分离站。 根据进一步的示例性系统和方法，可以使用超声波代替裂解剂或除裂解剂以裂解湿藻类生物质。
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公开(公告)号：US08865452B2
公开(公告)日：2014-10-21
申请号：US12485027
申请日：2009-06-15
申请人： Guido Radaelli , Daniel Fleischer
发明人： Guido Radaelli , Daniel Fleischer
CPC分类号： C12P7/6463 , C12M21/02 , C12M47/06 , C12N1/066
摘要： Presented herein are exemplary systems and methods for extracting lipids from a wet algal biomass. An exemplary method comprises lysing a wet algal biomass with an insoluble granular lysing agent to create a lysate, creating a lipid-rich phase in the lysate, and separating the lipid-rich phase from the lysate. An exemplary system comprises a lysing station for creating a lysate from a wet algal biomass and a separation station for creating and separating a lipid-rich phase from the lysate. According to further exemplary systems and methods, ultrasound may be used in place of or in addition to a lysing agent to lyse the wet algal biomass.
摘要翻译： 本文提出的是用于从湿藻类生物质中提取脂质的示例性系统和方法。 一个示例性的方法包括用不溶性颗粒状裂解剂裂解湿藻类生物质以产生裂解物，在裂解物中产生富含脂质的相，并将富脂相与裂解物分离。 示例性系统包括用于从湿藻类生物质产生裂解物的裂解站和用于从裂解物中产生和分离富脂相的分离站。 根据进一步的示例性系统和方法，可以使用超声波代替裂解剂或除裂解剂以裂解湿藻类生物质。
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