Nucleic acid isolation
    4.
    发明申请
    Nucleic acid isolation 审中-公开
    核酸分离

    公开(公告)号:US20070122809A1

    公开(公告)日:2007-05-31

    申请号:US10565951

    申请日:2004-07-21

    IPC分类号: C12Q1/68 C07H21/04

    CPC分类号: C12N15/1017

    摘要: The invention provides materials and methods for purifying nucleic acids from a biological sample held in a container (30) such as a centrifuge tube. A filtration unit (1) having a filter (7, 9) and a filtrate chamber (5) is placed within the container (30). Preferably the exterior of the filtration unit (1) engages sealingly with the interior surface of the container (30). Relative movement between the container (30) and the filtration unit (1) causes the sample to pass through the filter (7, 9) into the filtrate chamber (5), where it can be further manipulated. In preferred embodiments the filtrate is then contacted with a charge-switch nucleic acid binding agent in order to purify the nucleic acid further.

    摘要翻译: 本发明提供从保存在容器(30)如离心管中的生物样品中提纯核酸的材料和方法。 将具有过滤器(7,9)和滤液室(5)的过滤单元(1)放置在容器(30)内。 优选地,过滤单元(1)的外部密封地与容器(30)的内​​表面接合。 容器(30)和过滤单元(1)之间的相对运动使得样品通过过滤器(7,9)进入滤液室(5),在那里可以进一步操作。 在优选的实施方案中,然后使滤液与电荷开关核酸结合剂接触,以便进一步纯化核酸。

    Separation of nucleic acid
    5.
    发明申请
    Separation of nucleic acid 审中-公开
    分离核酸

    公开(公告)号:US20060024712A1

    公开(公告)日:2006-02-02

    申请号:US11167907

    申请日:2005-06-27

    IPC分类号: C12Q1/68 C12N1/08

    摘要: Compositions, methods and kits for separating nucleic acid from cell samples. Cells are lysed and nuclear material is flocculated/precipitated. Genomic DNA can be collected from the precipitate and purified. RNA present in the supernatant can be collected (e.g., bound to a solid phase) and purified.

    摘要翻译: 用于从细胞样品中分离核酸的组合物,方法和试剂盒。 细胞裂解,核物质被絮凝/沉淀。 可从沉淀物中收集基因组DNA并纯化。 可以收集存在于上清液中的RNA(例如,结合到固相)并纯化。