公开(公告)号：US20050233301A1

公开(公告)日：2005-10-20

申请号：US11009192

申请日：2004-12-09

申请人： David Berd ,  Lorne Erdile ,  Margalit Mokyr

发明人： David Berd ,  Lorne Erdile ,  Margalit Mokyr

IPC分类号： C12N1/04 ,  A01N20060101 ,  A01N1/00 ,  A01N1/02 ,  A61K9/19 ,  A61K35/12 ,  A61K39/00 ,  A61K39/39 ,  A61K47/26 ,  A61K47/42 ,  C12N5/06 ,  C12N5/08

CPC分类号： A01N1/0221 ,  A01N1/02

摘要： A method of preserving haptenized tumor cells is described. The method employs a freezing medium containing an effective amount of sucrose and human serum albumin in an isotonic buffered saline solution. Cryogenically preserving haptenized cells in such a medium has been found to maintain the integrity of the tumor cells during storage. The haptenized tumor cells also retain cell-associated antigens and haptens, and are as immunogenic, i.e., capable of inducing immunotherapeutic response, as fresh vaccine in a mouse model of metastatic disease. In a specific embodiment, haptenized cells are exposed to a solution of 8% sucrose, 10% human serum albumin in Hank's buffered solution, and then frozen to −80° C. overnight and then stored in a liquid nitrogen freezer. Methods of storing haptenized tumor cells and compositions are also provided.

摘要翻译： 描述了保留半抗原的肿瘤细胞的方法。 该方法在等渗缓冲盐水溶液中使用含有有效量的蔗糖和人血清白蛋白的冷冻介质。 已经发现在这种培养基中低温保存半抗原细胞在保存期间维持肿瘤细胞的完整性。 半抗原的肿瘤细胞还保留细胞相关抗原和半抗原，并且作为免疫原性，即能够诱导免疫治疗应答，作为转移性疾病的小鼠模型中的新鲜疫苗。 在一个具体实施方案中，将半抗原细胞暴露于Hank's缓冲溶液中的8％蔗糖，10％人血清白蛋白的溶液中，然后冷冻至-80℃过夜，然后储存在液氮冷冻器中。 还提供了存储半抗原化肿瘤细胞和组合物的方法。

公开(公告)号：US20070237795A1

公开(公告)日：2007-10-11

申请号：US11762409

申请日：2007-06-13

申请人： David Berd ,  Lorne Erdile ,  Margalit Mokyr

发明人： David Berd ,  Lorne Erdile ,  Margalit Mokyr

IPC分类号： A01N1/02 ,  A61K39/385

CPC分类号： A01N1/0221 ,  A01N1/02

摘要： A method of preserving haptenized tumor cells is described. The method employs a freezing medium containing an effective amount of sucrose and human serum albumin in an isotonic buffered saline solution. Cryogenically preserving haptenized cells in such a medium has been found to maintain the integrity of the tumor cells during storage. The haptenized tumor cells also retain cell-associated antigens and haptens, and are as immunogenic, i.e., capable of inducing immunotherapeutic response, as fresh vaccine in a mouse model of metastatic disease. In a specific embodiment, haptenized cells are exposed to a solution of 8% sucrose, 10% human serum albumin in Hank's buffered solution, and then frozen to −80° C. overnight and then stored in a liquid nitrogen freezer. Methods of storing haptenized tumor cells and compositions are also provided.

摘要翻译： 描述了保留半抗原的肿瘤细胞的方法。 该方法在等渗缓冲盐水溶液中使用含有有效量的蔗糖和人血清白蛋白的冷冻介质。 已经发现在这种培养基中低温保存半抗原细胞在保存期间维持肿瘤细胞的完整性。 半抗原的肿瘤细胞还保留细胞相关抗原和半抗原，并且作为免疫原性，即能够诱导免疫治疗应答，作为转移性疾病的小鼠模型中的新鲜疫苗。 在一个具体实施方案中，将半抗原细胞暴露于Hank's缓冲溶液中的8％蔗糖，10％人血清白蛋白的溶液中，然后冷冻至-80℃过夜，然后储存在液氮冷冻器中。 还提供了存储半抗原化肿瘤细胞和组合物的方法。

公开(公告)号：US20100221290A1

公开(公告)日：2010-09-02

申请号：US12775281

申请日：2010-05-06

申请人： David Berd

发明人： David Berd

IPC分类号： A61K39/00 ,  A61K35/12 ,  C12N5/00

CPC分类号： A61K39/0011 ,  A61K2039/5152 ,  A61K2039/55594 ,  A61K2039/6012

摘要： This invention relates to compositions comprising multi-haptenized tumor cells and extracts thereof, methods for preparing the compositions, vaccines comprising such multi-haptenized tumor cells, and methods for treating cancer with such vaccines. In a specific embodiment, melanoma and ovarian adenocarcinoma cells are multi-haptenized, wherein the tumor cells are differentially haptenized either with a dinitrophenyl group coupled to s-amino groups, or with a sulfanilic acid group coupled to aromatic side chains of histidine and tyrosine. A method of SA-haptenization is also provided.

摘要翻译： 本发明涉及包含多重半抗原化的肿瘤细胞及其提取物的组合物，制备组合物的方法，包含这种多元化的肿瘤细胞的疫苗以及用这种疫苗治疗癌症的方法。 在一个具体实施方案中，黑素瘤和卵巢腺癌细胞是多重半抗原化的，其中肿瘤细胞与偶联至s-氨基的二硝基苯基或与组氨酸和酪氨酸的芳族侧链偶联的对氨基苯磺酸基团差异半抗原化。 还提供了一种SA-半抗原化的方法。

公开(公告)号：US20080044441A1

公开(公告)日：2008-02-21

申请号：US11643626

申请日：2006-12-20

申请人： David Berd

发明人： David Berd

IPC分类号： A61K39/385 ,  A61P35/00 ,  C12N5/02

CPC分类号： A61K39/0011 ,  A61K2039/5152 ,  A61K2039/55594 ,  A61K2039/6012

摘要： This invention relates to compositions comprising multi-haptenized tumor cells and extracts thereof, methods for preparing the compositions, vaccines comprising such multi-haptenized tumor cells, and methods for treating cancer with such vaccines. In a specific embodiment, melanoma and ovarian adenocarcinoma cells are multi-haptenized, wherein the tumor cells are differentially haptenized either with a dinitrophenyl group coupled to ε-amino groups, or with a sulfanilic acid group coupled to aromatic side chains of histidine and tyrosine. A method of SA-haptenization is also provided.

摘要翻译： 本发明涉及包含多重半抗原化的肿瘤细胞及其提取物的组合物，制备组合物的方法，包含这种多元化的肿瘤细胞的疫苗以及用这种疫苗治疗癌症的方法。 在一个具体实施方案中，黑素瘤和卵巢腺癌细胞是多重半抗原化的，其中肿瘤细胞与偶联至ε-氨基的二硝基苯基或与组氨酸和酪氨酸的芳族侧链偶联的对位氨基酸差异半抗原化。 还提供了一种SA-半抗原化的方法。

公开(公告)号：US07585512B1

公开(公告)日：2009-09-08

申请号：US08203004

申请日：1994-02-28

申请人： David Berd

发明人： David Berd

IPC分类号： A61K39/00 ,  A61K39/385

CPC分类号： A61K39/0011 ,  A61K2039/5152 ,  A61K2039/55594 ,  A61K2039/6012 ,  A61K39/385 ,  A61K38/20 ,  A61K2300/00

摘要： The present invention is directed to a treatment for cancer. Compositions and methods of treating cancer are included in the scope of the present invention. The compositions of the present invention include a composition prepared from tumor cells or tumor cell extracts. The methods of the present invention are directed to treating cancer comprising administering a therapeutically effective amount of a composition comprising tumor cells and/or tumor cell extracts.

摘要翻译： 本发明涉及癌症治疗。 治疗癌症的组合物和方法包括在本发明的范围内。 本发明的组合物包括由肿瘤细胞或肿瘤细胞提取物制备的组合物。 本发明的方法涉及治疗癌症，包括施用治疗有效量的包含肿瘤细胞和/或肿瘤细胞提取物的组合物。

公开(公告)号：US20060240047A1

公开(公告)日：2006-10-26

申请号：US11331468

申请日：2006-01-13

申请人： David Berd

发明人： David Berd

IPC分类号： A61K39/00 ,  A61K31/66

CPC分类号： A61K39/385 ,  A61K39/0011 ,  A61K2039/5152 ,  A61K2039/55594

摘要： The present invention discloses a method for producing a haptenized vaccine from a tissue biopsy. The method includes obtaining a tissue biopsy, isolating the cells, irradiating the cells, haptenizing the cells, and cryopreserving the cells. The present invention also discloses a method for treating cancer using the vaccines produced by the methods described herein.

摘要翻译： 本发明公开了一种从组织活检中制备半抗原疫苗的方法。 该方法包括获得组织活组织检查，分离细胞，照射细胞，使细胞半反应，以及冷冻保存细胞。 本发明还公开了使用通过本文所述的方法产生的疫苗来治疗癌症的方法。

公开(公告)号：US06333028B1

公开(公告)日：2001-12-25

申请号：US09134465

申请日：1998-08-14

申请人： David Berd

发明人： David Berd

IPC分类号： A01N6300

CPC分类号： A61K39/0011 ,  A61K2039/5152 ,  A61K2039/55577 ,  A61K2039/55594 ,  A61K2039/6012

摘要： The present invention is directed to compositions and methods of treating cancer. The compositions of the present invention include a composition prepared from a tumor cell which is hapten modified and syngeneic to the type of tumor to be treated. The composition has the properties, when administered with an adjuvant to a human suffering from a malignant tumor of the same type as said tumor cell, of eliciting T lymphocytes that infiltrate the tumor of said human, of eliciting an inflammatory immune response against the tumor of said human, and of eliciting a delayed-type hypersensitivity response to the tumor of said human. The methods of the present invention are directed to treating cancer comprising administering a therapeutically effective amount of a composition comprising a tumor cell.

摘要翻译： 本发明涉及治疗癌症的组合物和方法。 本发明的组合物包括由肿瘤细胞制备的组合物，其被半抗原修饰并与待治疗的肿瘤类型是同基因的。 当向患有与所述肿瘤细胞相同类型的恶性肿瘤的人施用佐剂时，该组合物具有引起渗透所述人的肿瘤的T淋巴细胞引起针对肿瘤的炎性免疫应答的性质 说人，并且引起对所述人的肿瘤的延迟型超敏反应。 本发明的方法涉及治疗癌症，包括施用治疗有效量的包含肿瘤细胞的组合物。

公开(公告)号：US07560103B2

公开(公告)日：2009-07-14

申请号：US10025195

申请日：2001-12-19

申请人： David Berd

发明人： David Berd

IPC分类号： A61K45/00

CPC分类号： A61K39/0011 ,  A61K2039/5152 ,  A61K2039/55577 ,  A61K2039/55594 ,  A61K2039/6012

摘要： The present invention is directed to compositions and methods of treating cancer. The compositions of the present invention include a composition prepared from a tumor cell which is hapten modified and syngeneic to the type of tumor to be treated. The composition has the properties, when administered with an adjuvant to a human suffering from a malignant tumor of the same type as said tumor cell, of eliciting T lymphocytes that infiltrate the tumor of said human, of eliciting an inflammatory immune response against the tumor of said human, and of eliciting a delayed-type hypersensitivity response to the tumor of said human. The methods of the present invention are directed to treating cancer comprising administering a therapeutically effective amount of a composition comprising a tumor cell.

摘要翻译： 本发明涉及治疗癌症的组合物和方法。 本发明的组合物包括由肿瘤细胞制备的组合物，其被半抗原修饰并与待治疗的肿瘤类型是同基因的。 当向患有与所述肿瘤细胞相同类型的恶性肿瘤的人施用佐剂时，组合物具有引起渗透所述人的肿瘤的T淋巴细胞引起针对肿瘤的炎症免疫应答的性质 说人，并且引起对所述人的肿瘤的延迟型超敏反应。 本发明的方法涉及治疗癌症，包括施用治疗有效量的包含肿瘤细胞的组合物。

公开(公告)号：US20050232951A1

公开(公告)日：2005-10-20

申请号：US11015769

申请日：2004-12-17

申请人： David Berd

发明人： David Berd

IPC分类号： A61K39/00 ,  A61P35/00 ,  A61P37/04 ,  A61K48/00 ,  A61K31/28 ,  A61K31/353

CPC分类号： A61K39/0011 ,  A61K2039/5152 ,  A61K2039/55594 ,  A61K2039/6012

摘要： This invention relates to compositions comprising haptenized tumor cells and extracts thereof, methods for preparing the compositions, vaccines comprising such haptenized tumor cells, and methods for treating cancer with such vaccines. In a specific embodiment, melanoma cells are haptenized with a dinitrophenyl group, and used for treatment of melanoma patients having metastatic disease. Preferably, patients are given a first vaccine dose containing haptenized cells to “prime” the immune system. Subsequently, patients are injected with an immunomodulatory compound such as cyclophosphamide. In a preferred embodiment, an appropriate time period after the “priming” vaccine dose, additional vaccine doses containing a mixture of haptenized cells and an adjuvant are administered. The described treatment plan is more effective for eliciting favorable anti-tumor immune responses.

摘要翻译： 本发明涉及包含半抗原化肿瘤细胞及其提取物的组合物，制备组合物的方法，包含这种半抗原化肿瘤细胞的疫苗以及用这种疫苗治疗癌症的方法。 在一个具体实施方案中，黑素瘤细胞用二硝基苯基进行半抗原化，并用于治疗具有转移性疾病的黑素瘤患者。 优选地，患者被给予含有半抗原化细胞以“引发”免疫系统的第一疫苗剂量。 随后，向患者注射免疫调节化合物如环磷酰胺。 在优选的实施方案中，施用“引发”疫苗剂量后的适当时间段，施用含有半抗原化细胞和佐剂混合物的另外的疫苗剂量。 所描述的治疗计划对于引发有利的抗肿瘤免疫应答更有效。

公开(公告)号：US20140113371A1

公开(公告)日：2014-04-24

申请号：US13888024

申请日：2013-05-06

申请人： David Berd ,  Lorne F. Erdile ,  Margalit B. Mokyr

发明人： David Berd ,  Lorne F. Erdile ,  Margalit B. Mokyr

IPC分类号： A01N1/02

CPC分类号： A01N1/0221 ,  A01N1/02

摘要： A method of preserving haptenized tumor cells is described. The method employs a freezing medium containing an effective amount of sucrose and human serum albumin in an isotonic buffered saline solution. Cryogenically preserving haptenized cells in such a medium has been found to maintain the integrity of the tumor cells during storage. The haptenized tumor cells also retain cell-associated antigens and haptens, and are as immunogenic, i.e., capable of inducing immunotherapeutic response, as fresh vaccine in a mouse model of metastatic disease. In a specific embodiment, haptenized cells are exposed to a solution of 8% sucrose, 10% human serum albumin in Hank's buffered solution, and then frozen to −80° C. overnight and then stored in a liquid nitrogen freezer. Methods of storing haptenized tumor cells and compositions are also provided.

摘要翻译： 描述了保留半抗原的肿瘤细胞的方法。 该方法在等渗缓冲盐水溶液中使用含有有效量的蔗糖和人血清白蛋白的冷冻介质。 已经发现在这种培养基中低温保存半抗原细胞在保存期间维持肿瘤细胞的完整性。 半抗原的肿瘤细胞还保留细胞相关抗原和半抗原，并且作为免疫原性，即能够诱导免疫治疗应答，作为转移性疾病的小鼠模型中的新鲜疫苗。 在一个具体实施方案中，将半抗原细胞暴露于Hank's缓冲溶液中的8％蔗糖，10％人血清白蛋白的溶液中，然后冷冻至-80℃过夜，然后储存在液氮冷冻器中。 还提供了存储半抗原化肿瘤细胞和组合物的方法。