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12 条记录 (耗时 0.023 秒)

    Cryopreservation of haptenized tumor cells
    2.
    发明申请
    Cryopreservation of haptenized tumor cells 审中-公开
    半抗原肿瘤细胞的冷冻保存

    公开(公告)号：US20050233301A1

    公开(公告)日：2005-10-20

    申请号：US11009192

    申请日：2004-12-09

    申请人： David Berd ,  Lorne Erdile ,  Margalit Mokyr

    发明人： David Berd ,  Lorne Erdile ,  Margalit Mokyr

    IPC分类号： C12N1/04 ,  A01N20060101 ,  A01N1/00 ,  A01N1/02 ,  A61K9/19 ,  A61K35/12 ,  A61K39/00 ,  A61K39/39 ,  A61K47/26 ,  A61K47/42 ,  C12N5/06 ,  C12N5/08

    CPC分类号： A01N1/0221 ,  A01N1/02

    摘要： A method of preserving haptenized tumor cells is described. The method employs a freezing medium containing an effective amount of sucrose and human serum albumin in an isotonic buffered saline solution. Cryogenically preserving haptenized cells in such a medium has been found to maintain the integrity of the tumor cells during storage. The haptenized tumor cells also retain cell-associated antigens and haptens, and are as immunogenic, i.e., capable of inducing immunotherapeutic response, as fresh vaccine in a mouse model of metastatic disease. In a specific embodiment, haptenized cells are exposed to a solution of 8% sucrose, 10% human serum albumin in Hank's buffered solution, and then frozen to −80° C. overnight and then stored in a liquid nitrogen freezer. Methods of storing haptenized tumor cells and compositions are also provided.

    摘要翻译： 描述了保留半抗原的肿瘤细胞的方法。 该方法在等渗缓冲盐水溶液中使用含有有效量的蔗糖和人血清白蛋白的冷冻介质。 已经发现在这种培养基中低温保存半抗原细胞在保存期间维持肿瘤细胞的完整性。 半抗原的肿瘤细胞还保留细胞相关抗原和半抗原，并且作为免疫原性，即能够诱导免疫治疗应答，作为转移性疾病的小鼠模型中的新鲜疫苗。 在一个具体实施方案中，将半抗原细胞暴露于Hank's缓冲溶液中的8％蔗糖，10％人血清白蛋白的溶液中，然后冷冻至-80℃过夜，然后储存在液氮冷冻器中。 还提供了存储半抗原化肿瘤细胞和组合物的方法。

    Restenosis/atherosclerosis diagnosis, prophylaxis and therapy
    4.
    发明授权
    Restenosis/atherosclerosis diagnosis, prophylaxis and therapy 失效
    再狭窄/动脉粥样硬化诊断，预防和治疗

    公开(公告)号：US06183752B2

    公开(公告)日：2001-02-06

    申请号：US08796101

    申请日：1997-02-05

    申请人： Stephen E. Epstein ,  Toren Finkel ,  Edith Speir ,  Yi Fu Zhou ,  Jianhui Zhu ,  Lorne Erdile ,  Steven Pincus

    发明人： Stephen E. Epstein ,  Toren Finkel ,  Edith Speir ,  Yi Fu Zhou ,  Jianhui Zhu ,  Lorne Erdile ,  Steven Pincus

    IPC分类号： A61K39245

    CPC分类号： C12Q1/6883 ,  A61K39/00 ,  A61K39/0011 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/58 ,  C07K14/005 ,  C07K14/4746 ,  C07K2319/00 ,  C12N2710/14143 ,  C12N2710/16122 ,  C12N2710/16134 ,  C12Q2600/156

    摘要： Disclosed and claimed are compositions and methods for therapy and/or prevention of restenosis and/or atherosclerosis. The compositions can include an agent for decreasing viral load of cytomegalovirus, such as an immunological composition or vaccine against cytomegalovirus (CMV) containing at least one epitope of interest of CMV and/or an expression system which expresses at least one epitope of interest of CMV. Such compositions can include at least one epitope of p53. Alternatively, the compositions can include at least one epitope of p53 and/or an expression system which expresses the epitope. The methods can include administering the compositions to a patient in need of such therapy and/or prevention. Additionally, compositions and methods for diagnosing atherosclerosis and/or restenosis, or susceptibility thereto, including screening a sample from a patient for antibodies to CMV and/or CMV proteins and/or screening a sample from a patient for specific viral proteins that predict whether the virus has been reactivated and/or antibodies thereto and/or detecting whether CMV nucleic acid, e.g., mRNA is present in peripheral blood monocytes (PBMCs) and/or detecting a cellular-mediated immune response to CMV peptides or proteins is present and/or HLA phenotyping and/or HLA genotyping. Embodiements can include a skin test.

    摘要翻译： 公开并要求保护的是用于治疗和/或预防再狭窄和/或动脉粥样硬化的组合物和方法。 组合物可以包括用于降低巨细胞病毒的病毒载量的试剂，例如针对巨细胞病毒（CMV）的免疫组合物或疫苗，其包含至少一种CMV感兴趣的表位和/或表达至少一种CMV感兴趣的表位的表达系统 。 这样的组合物可以包括p53的至少一个表位。 或者，组合物可以包括p53的至少一个表位和/或表达表位的表达系统。 所述方法可以包括将组合物施用于需要这种治疗和/或预防的患者。 另外，用于诊断动脉粥样硬化和/或再狭窄或其易感性的组合物和方法，包括从患者筛选来自CMV和/或CMV蛋白的抗体的样品和/或筛选来自患者的特定病毒蛋白的样品，所述特定病毒蛋白预测是否 病毒已被重新激活和/或其抗体和/或检测CMV核酸（例如mRNA）是否存在于外周血单核细胞（PBMC）中和/或检测对CMV肽或蛋白质的细胞介导的免疫应答存在和/或 HLA表型和/或HLA基因分型。 身体可以包括皮肤测试。

    Cryopreservation of Haptenized Tumor Cells
    9.
    发明申请
    Cryopreservation of Haptenized Tumor Cells 失效
    Haptenized肿瘤细胞的冷冻保存

    公开(公告)号：US20070237795A1

    公开(公告)日：2007-10-11

    申请号：US11762409

    申请日：2007-06-13

    申请人： David Berd ,  Lorne Erdile ,  Margalit Mokyr

    发明人： David Berd ,  Lorne Erdile ,  Margalit Mokyr

    IPC分类号： A01N1/02 ,  A61K39/385

    CPC分类号： A01N1/0221 ,  A01N1/02

    摘要： A method of preserving haptenized tumor cells is described. The method employs a freezing medium containing an effective amount of sucrose and human serum albumin in an isotonic buffered saline solution. Cryogenically preserving haptenized cells in such a medium has been found to maintain the integrity of the tumor cells during storage. The haptenized tumor cells also retain cell-associated antigens and haptens, and are as immunogenic, i.e., capable of inducing immunotherapeutic response, as fresh vaccine in a mouse model of metastatic disease. In a specific embodiment, haptenized cells are exposed to a solution of 8% sucrose, 10% human serum albumin in Hank's buffered solution, and then frozen to −80° C. overnight and then stored in a liquid nitrogen freezer. Methods of storing haptenized tumor cells and compositions are also provided.

    摘要翻译： 描述了保留半抗原的肿瘤细胞的方法。 该方法在等渗缓冲盐水溶液中使用含有有效量的蔗糖和人血清白蛋白的冷冻介质。 已经发现在这种培养基中低温保存半抗原细胞在保存期间维持肿瘤细胞的完整性。 半抗原的肿瘤细胞还保留细胞相关抗原和半抗原，并且作为免疫原性，即能够诱导免疫治疗应答，作为转移性疾病的小鼠模型中的新鲜疫苗。 在一个具体实施方案中，将半抗原细胞暴露于Hank's缓冲溶液中的8％蔗糖，10％人血清白蛋白的溶液中，然后冷冻至-80℃过夜，然后储存在液氮冷冻器中。 还提供了存储半抗原化肿瘤细胞和组合物的方法。