公开(公告)号：US20090222944A1

公开(公告)日：2009-09-03

申请号：US12160287

申请日：2007-01-04

Applicant: Markus Frank ,  Patrick Schweizer ,  Dimitar Douchkov

Inventor： Markus Frank ,  Patrick Schweizer ,  Dimitar Douchkov

IPC: C12N15/82 ,  C12N15/11 ,  C12N15/00 ,  C12N5/04 ,  A01H5/00

CPC classification number: C07K14/415 ,  C12N15/8218 ,  C12N15/8282

Abstract: The invention relates to a method of generating or increasing a pathogen resistance in plants by reducing the expression of at least one stomatin polypeptide or a functional equivalent thereof. The invention relates to novel nucleic acid sequences coding for a Hordeum vulgare stomatin (HvSTM1) polynucleotide and describes homologous sequences (STM1) thereof, and to their use in methods for obtaining a pathogen resistance in plants, and to nucleic acid constructs, expression cassettes and vectors which comprise these sequences and which are suitable for mediating a fungal resistance in plants. The invention furthermore relates to transgenic organisms, in particular plants, which are transformed with these expression cassettes or vectors, and to cultures, parts or transgenic propagation material derived therefrom.

Abstract translation: 本发明涉及通过减少至少一种口油素多肽或其功能等同物的表达来产生或增加植物中病原体抗性的方法。 本发明涉及编码大麦草（HvSTM1）多核苷酸的新型核酸序列，并描述了它们的同源序列（STM1）及其在植物中获得病原体抗性的方法中的用途以及核酸构建体，表达盒和 包含这些序列并且适于介导植物中真菌抗性的载体。 本发明还涉及用这些表达盒或载体转化的转基因生物，特别是植物，以及由其衍生的培养物，部分或转基因繁殖材料。

公开(公告)号：US08362323B2

公开(公告)日：2013-01-29

申请号：US12092877

申请日：2006-10-27

Applicant: Dimitar Douchkov ,  Patrick Schweizer ,  Uwe Zierold

Inventor： Dimitar Douchkov ,  Patrick Schweizer ,  Uwe Zierold

IPC: A01H1/00 ,  C12N15/82 ,  C07H21/04

CPC classification number: C12N15/8279 ,  C12N15/8282

Abstract: The invention relates to a method of generating or increasing a pathogen resistance in plants by reducing the expression of at least one Armadillo repeat polypeptide or a functional equivalent thereof. The invention relates to novel nucleic acid sequences coding for a Hordeum vulgare Armadillo repeat (HvARM) polynucleotide and describes homologous sequences (ARM1) thereof, and to their use in methods for obtaining a pathogen resistance in plants e, and to nucleic acid constructs, expression cassettes and vectors which comprise these sequences and which are suitable for mediating a fungal resistance in plants. The invention furthermore relates to transgenic organisms, in particular plants, which are transformed with these expression cassettes or vectors, and to cultures, parts or transgenic propagation material derived therefrom.

Abstract translation: 本发明涉及通过减少至少一种犰狳重复多肽或其功能等同物的表达来产生或增加植物中病原体抗性的方法。 本发明涉及编码大麦禾本科犰狳重复（HvARM）多核苷酸的新型核酸序列，并描述其同源序列（ARM1）及其在植物中获得病原体抗性的方法和用于核酸构建体，表达 盒和载体，其包含这些序列并且适于介导植物中的真菌抗性。 本发明还涉及用这些表达盒或载体转化的转基因生物，特别是植物，以及由其衍生的培养物，部分或转基因繁殖材料。

公开(公告)号：US20100011467A1

公开(公告)日：2010-01-14

申请号：US12438766

申请日：2007-08-22

Applicant: Dimitar Douchkov ,  Patrick Schweizer

Inventor： Dimitar Douchkov ,  Patrick Schweizer

IPC: A01H5/00 ,  C12N15/82 ,  C12N15/29 ,  C07K14/00 ,  C12N5/10

CPC classification number: C07K14/415 ,  C12N9/1205 ,  C12N15/8282

Abstract: The present invention relates to a method of increasing the pathogen resistance in transgenic plants and/or plant cells, where a DNA sequence which codes for a protein with a leucine-rich-repeat (LRR) domain and/or a kinase activity is introduced into the plant or plant cell and expressed therein. The present invention also relates to the use of nucleic acids which code for such a protein, for the generation of transgenic plants or plant cells with an increased pathogen resistance. The present invention furthermore relates to nucleic acid sequences which code for a protein which confers an increased pathogen resistance in plants.

Abstract translation: 本发明涉及增加转基因植物和/或植物细胞中病原体抗性的方法，其中将编码具有富含亮氨酸重复（LRR）结构域和/或激酶活性的蛋白质的DNA序列引入 植物或植物细胞并在其中表达。 本发明还涉及编码这种蛋白质的核酸用于产生具有增加的病原体抗性的转基因植物或植物细胞的用途。 本发明还涉及编码赋予植物中增加的病原体抗性的蛋白质的核酸序列。

公开(公告)号：US08592652B2

公开(公告)日：2013-11-26

申请号：US12523101

申请日：2008-01-15

Applicant: Markus Frank ,  Patrick Schweizer ,  Dimitar Douchkov

Inventor： Markus Frank ,  Patrick Schweizer ,  Dimitar Douchkov

IPC: C12N15/82 ,  C12N5/04 ,  C12N15/90

CPC classification number: C12N15/8282 ,  C12N9/50

Abstract: The invention relates to a method of generating or increasing a pathogen resistance in plants by reducing the expression of at least one subtilisin polypeptide or a functional equivalent thereof. The invention relates to novel nucleic acid sequences coding for a Hordeum vulgare subtilisin (HvRNR9) and Triticum aestivum subtilisin (TaRNR9) polynucleotide and describes homologous sequences (RNR9) thereof, and to their use in methods for obtaining a pathogen resistance in plants, and to nucleic acid constructs, expression cassettes and vectors which comprise these sequences and which are suitable for mediating a fungal resistance in plants. The invention furthermore relates to transgenic organisms, in particular plants, which are transformed with these expression cassettes or vectors, and to cultures, parts or transgenic propagation material derived therefrom.

Abstract translation: 本发明涉及通过减少至少一种枯草杆菌蛋白酶多肽或其功能等同物的表达来产生或增加植物中病原体抗性的方法。 本发明涉及编码大麦草枯草杆菌蛋白酶（HvRNR9）和小麦枯草杆菌蛋白酶（TaRNR9）多核苷酸的新型核酸序列，并描述其同源序列（RNR9）及其在获得植物病原体抗性的方法中的用途，以及 核酸构建体，表达盒和载体，其包含这些序列并且适于介导植物中的真菌抗性。 本发明还涉及用这些表达盒或载体转化的转基因生物，特别是植物，以及由其衍生的培养物，部分或转基因繁殖材料。

公开(公告)号：US08329988B2

公开(公告)日：2012-12-11

申请号：US12444927

申请日：2007-10-12

Applicant: Markus Frank ,  Patrick Schweizer ,  Dimitar Douchkov

Inventor： Markus Frank ,  Patrick Schweizer ,  Dimitar Douchkov

IPC: A01H5/00 ,  A01H5/10 ,  C12N15/09 ,  C12N15/82

CPC classification number: C12N15/8282 ,  C07K14/415

Abstract: The invention relates to a method of increasing the resistance to one or more penetrating pathogen(s) in a monocotyledonous or dicotyledonous plant or a part of a plant, for example in an organ, tissue, a cell or a part of a plant cell, for example in an organell, wherein a DNA sequence which codes for an armadillo repeat ARM1 protein and which mediates an increased pathogen resistance, preferably an increased resistance to fungal pathogens, is increased into, and expressed in, the plant or plant cell; or wherein an endogenous DNA sequence which codes for an armadillo repeat ARM1 protein and which mediates an increased pathogen resistance, preferably an increased resistance to fungal pathogens, is increased in the plant or plant cell in comparison with the original, or wild-type, plant, or wherein the endogenous gene sequence or preferably the 5′-untranslated region (5′UTR) is modified in comparison with the original sequence. The invention also relates to plants, to parts of a plant, for example an organ, tissue, a cell or a part of a plant cell, for example an organelle, which are obtained by the above methods, and to corresponding propagation material.

Abstract translation: 本发明涉及增加对单子叶植物或双子叶植物或植物的一部分，例如在器官，组织，细胞或植物细胞的一部分中的一种或多种穿透性病原体的抗性的方法， 例如在细胞器中，其中编码犰狳重复ARM1蛋白并且介导增加的病原体抗性，优选增加的对真菌病原体的抗性的DNA序列增加到植物或植物细胞中并在其中表达; 或者其中与原始或野生型植物相比，在植物或植物细胞中增加编码犰狳重复ARM1蛋白并且介导增加的病原体抗性，优选增加的对真菌病原体的抗性的内源DNA序列 ，或其中内源性基因序列或优选5'-非翻译区（5'UTR）与原始序列相比被修饰。 本发明还涉及植物，例如通过上述方法获得的植物部分，例如器官，组织，细胞或植物细胞的一部分，例如细胞器，以及相应的繁殖材料。

公开(公告)号：US20090241215A1

公开(公告)日：2009-09-24

申请号：US12092877

申请日：2006-10-27

Applicant: Dimitar Douchkov ,  Patrick Schweizer ,  Uwe Zierold

Inventor： Dimitar Douchkov ,  Patrick Schweizer ,  Uwe Zierold

IPC: C12N15/82 ,  C12N15/11 ,  C07K14/00 ,  C12N15/00 ,  C12N5/04 ,  A01H5/00

CPC classification number: C12N15/8279 ,  C12N15/8282

Abstract: The invention relates to a method of generating or increasing a pathogen resistance in plants by reducing the expression of at least one Armadillo repeat polypeptide or a functional equivalent thereof. The invention relates to novel nucleic acid sequences coding for a Hordeum vulgare Armadillo repeat (HvARM) polynucleotide and describes homologous sequences (ARM1) thereof, and to their use in methods for obtaining a pathogen resistance in plants e, and to nucleic acid constructs, expression cassettes and vectors which comprise these sequences and which are suitable for mediating a fungal resistance in plants. The invention furthermore relates to transgenic organisms, in particular plants, which are transformed with these expression cassettes or vectors, and to cultures, parts or transgenic propagation material derived therefrom.

Abstract translation: 本发明涉及通过减少至少一种犰狳重复多肽或其功能等同物的表达来产生或增加植物中病原体抗性的方法。 本发明涉及编码大麦禾本科犰狳重复（HvARM）多核苷酸的新型核酸序列，并描述其同源序列（ARM1）及其在植物中获得病原体抗性的方法和用于核酸构建体，表达 盒和载体，其包含这些序列并且适于介导植物中的真菌抗性。 本发明还涉及用这些表达盒或载体转化的转基因生物，特别是植物，以及由其衍生的培养物，部分或转基因繁殖材料。

公开(公告)号：US08222486B2

公开(公告)日：2012-07-17

申请号：US12438766

申请日：2007-08-22

Applicant: Dimitar Douchkov ,  Patrick Schweizer

Inventor： Dimitar Douchkov ,  Patrick Schweizer

IPC: C12N15/09 ,  C12N15/29 ,  C12N15/82

CPC classification number: C07K14/415 ,  C12N9/1205 ,  C12N15/8282

Abstract: The present invention relates to a method of increasing the pathogen resistance in transgenic plants and/or plant cells, where a DNA sequence which codes for a protein with a leucine-rich-repeat (LRR) domain and/or a kinase activity is introduced into the plant or plant cell and expressed therein. The present invention also relates to the use of nucleic acids which code for such a protein, for the generation of transgenic plants or plant cells with an increased pathogen resistance. The present invention furthermore relates to nucleic acid sequences which code for a protein which confers an increased pathogen resistance in plants.

Abstract translation: 本发明涉及增加转基因植物和/或植物细胞中病原体抗性的方法，其中将编码具有富含亮氨酸重复（LRR）结构域和/或激酶活性的蛋白质的DNA序列引入 植物或植物细胞并在其中表达。 本发明还涉及编码这种蛋白质的核酸用于产生具有增加的病原体抗性的转基因植物或植物细胞的用途。 本发明还涉及编码赋予植物中增加的病原体抗性的蛋白质的核酸序列。

公开(公告)号：US08178751B2

公开(公告)日：2012-05-15

申请号：US12160287

申请日：2007-01-04

Applicant: Markus Frank ,  Patrick Schweizer ,  Dimitar Douchkov

Inventor： Markus Frank ,  Patrick Schweizer ,  Dimitar Douchkov

IPC: C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00

CPC classification number: C07K14/415 ,  C12N15/8218 ,  C12N15/8282

Abstract: The invention relates to a method of generating or increasing a pathogen resistance in plants by reducing the expression of at least one stomatin polypeptide or a functional equivalent thereof. The invention relates to novel nucleic acid sequences coding for a Hordeum vulgare stomatin (HvSTM1) polynucleotide and describes homologous sequences (STM1) thereof, and to their use in methods for obtaining a pathogen resistance in plants, and to nucleic acid constructs, expression cassettes and vectors which comprise these sequences and which are suitable for mediating a fungal resistance in plants. The invention furthermore relates to transgenic organisms, in particular plants, which are transformed with these expression cassettes or vectors, and to cultures, parts or transgenic propagation material derived therefrom.

Abstract translation: 本发明涉及通过减少至少一种口油素多肽或其功能等同物的表达来产生或增加植物中病原体抗性的方法。 本发明涉及编码大麦草（HvSTM1）多核苷酸的新型核酸序列，并描述了它们的同源序列（STM1）及其在植物中获得病原体抗性的方法中的用途以及核酸构建体，表达盒和 包含这些序列并且适于介导植物中真菌抗性的载体。 本发明还涉及用这些表达盒或载体转化的转基因生物，特别是植物，以及由其衍生的培养物，部分或转基因繁殖材料。

公开(公告)号：US20100170005A1

公开(公告)日：2010-07-01

申请号：US12523101

申请日：2008-01-15

Applicant: Markus Frank ,  Patrick Schweizer ,  Dimitar Douchkov

Inventor： Markus Frank ,  Patrick Schweizer ,  Dimitar Douchkov

IPC: A01H5/00 ,  C12N15/82 ,  C07H21/00 ,  C12N15/74 ,  C07H21/02 ,  C12N1/21

CPC classification number: C12N15/8282 ,  C12N9/50

Abstract: The invention relates to a method of generating or increasing a pathogen resistance in plants by reducing the expression of at least one subtilisin polypeptide or a functional equivalent thereof. The invention relates to novel nucleic acid sequences coding for a Hordeum vulgare subtilisin (HvRNR9) and Triticum aestivum subtilisin (TaRNR9) polynucleotide and describes homologous sequences (RNR9) thereof, and to their use in methods for obtaining a pathogen resistance in plants, and to nucleic acid constructs, expression cassettes and vectors which comprise these sequences and which are suitable for mediating a fungal resistance in plants. The invention furthermore relates to transgenic organisms, in particular plants, which are transformed with these expression cassettes or vectors, and to cultures, parts or transgenic propagation material derived therefrom.

Abstract translation: 本发明涉及通过减少至少一种枯草杆菌蛋白酶多肽或其功能等同物的表达来产生或增加植物中病原体抗性的方法。 本发明涉及编码大麦草枯草杆菌蛋白酶（HvRNR9）和小麦枯草杆菌蛋白酶（TaRNR9）多核苷酸的新型核酸序列，并描述其同源序列（RNR9）及其在获得植物病原体抗性的方法中的用途，以及 核酸构建体，表达盒和载体，其包含这些序列并且适于介导植物中的真菌抗性。 本发明还涉及用这些表达盒或载体转化的转基因生物，特别是植物，以及由其衍生的培养物，部分或转基因繁殖材料。

公开(公告)号：US20100071089A1

公开(公告)日：2010-03-18

申请号：US12444927

申请日：2007-10-12

Applicant: Markus Frank ,  Patrick Schweizer ,  Dimitar Douchkov

Inventor： Markus Frank ,  Patrick Schweizer ,  Dimitar Douchkov

IPC: C12N15/82 ,  A01H5/00 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10

CPC classification number: C12N15/8282 ,  C07K14/415

Abstract: The invention relates to a method of increasing the resistance to one or more penetrating pathogen(s) in a monocotyledonous or dicotyledonous plant or a part of a plant, for example in an organ, tissue, a cell or a part of a plant cell, for example in an organell, wherein a DNA sequence which codes for an armadillo repeat ARM1 protein and which mediates an increased pathogen resistance, preferably an increased resistance to fungal pathogens, is increased into, and expressed in, the plant or plant cell; or wherein an endogenous DNA sequence which codes for an armadillo repeat ARM1 protein and which mediates an increased pathogen resistance, preferably an increased resistance to fungal pathogens, is increased in the plant or plant cell in comparison with the original, or wild-type, plant, or wherein the endogenous gene sequence or preferably the 5′-untranslated region (5′UTR) is modified in comparison with the original sequence. The invention also relates to plants, to parts of a plant, for example an organ, tissue, a cell or a part of a plant cell, for example an organelle, which are obtained by the above methods, and to corresponding propagation material.

Abstract translation: 本发明涉及增加对单子叶植物或双子叶植物或植物的一部分，例如在器官，组织，细胞或植物细胞的一部分中的一种或多种穿透性病原体的抗性的方法， 例如在细胞器中，其中编码犰狳重复ARM1蛋白并且介导增加的病原体抗性，优选增加的对真菌病原体的抗性的DNA序列增加到植物或植物细胞中并在其中表达; 或者其中与原始或野生型植物相比，在植物或植物细胞中增加编码犰狳重复ARM1蛋白并且介导增加的病原体抗性，优选增加的对真菌病原体的抗性的内源DNA序列 ，或其中内源性基因序列或优选5'-非翻译区（5'UTR）与原始序列相比被修饰。 本发明还涉及植物，例如通过上述方法获得的植物部分，例如器官，组织，细胞或植物细胞的一部分，例如细胞器，以及相应的繁殖材料。